SBI-115-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemESBI-115Cat.No.:HY-111534CASNo.:882366-16-7分?式:C??H??ClN?O?S分?量:340.78作?靶點:Gprotein-coupledBileAcidReceptor1作?通路:GPCR/GProtein儲存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實驗DMSO:150mg/mL(440.17mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲備液1mM2.9344mL14.6722mL29.3444mL5mM0.5869mL2.9344mL5.8689mL10mM0.2934mL1.4672mL2.9344mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；?旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲存時，請在6個?內(nèi)使?，-20°C儲存時，請在1個?內(nèi)使?。體內(nèi)實驗請根據(jù)您的實驗動物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請先按照InVitro?式配制澄的儲備液，再依次添加助溶劑：(為保證實驗結(jié)果的可靠性，澄的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實驗的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的?式助溶)1.請依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.5mg/mL(7.34mM);Clearsolution1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE2.請依序添加每種溶劑：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(7.34mM);ClearsolutionBIOLOGICALACTIVITY?物活性SBI-115?種TGR5(GPCR19)拮抗劑。SBI-115通過抑制TGR5降低多囊性肝病的肝囊腫形成[1]。體外研究SBI-115(100-200μM,24hours)inhibitsproliferationtriggeredbypre-treatmentofcysticcholangiocyteswithTaurolithocholicacid(TLCA)inshRNA-transfectedADPKDcholangiocytes[1].CellProliferationAssay[1]CellLine:shRNA-transfectedADPKDcholangiocytesConcentration:100,200μMIncubationTime:24hoursResult:Inhibitedproliferation(by32-48%)triggeredbypre-treatmentofcysticcholangiocyteswithTLCA.戶使?本產(chǎn)品發(fā)表的科研?獻?NatCommun.2022Oct14;13(1):6081.?ActaPharmSinB.21July2021.?PharmacolRes.2022Sep21;106459.?iScience.2021,102508.?AquacultRep.February2022,100997.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].MasyukTV,etal.TGR5contributestohepaticcystogenesisinrodentswithpolycysticliverdiseasesthroughcyclicadenosinemonophosphate/Gαssignaling.Hepatology.2017Oct;66(4):1197-1218.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel