有機(jī)磷農(nóng)藥檢測(cè)（英文）

PesticideresidueWhatisapesticide?Pesticidemeansanysubstanceintendedfor

preventing,destroying,attracting,repelling,orcontrollinganypestincludingunwantedspeciesofplantsoranimalsduringtheproduction,storage,transport,distribution,andprocessingoffood,agriculturalcommodities,oranimalfeedsorwhichmaybeadministeredtoanimalsforthecontrolofectoparasites.

Thetermincludessubstancesintendedforuseasaplant-growthregulator,defoliant,desiccant,fruitthinningagent,orsproutinginhibitorSubstancesappliedtocropseitherbeforeorafterharvesttoprotectthecommodityfromdeteriorationduringstorageandtransportAccordingtotheactiveelement,thepesticideincludeorganochlorinepesticideorganophosphorouspesticidecarbamatepesticidesyntheticpyrethroidpesticideothersthepesticidecanexistinAir:sprypesticideWater:whenwashthepesticidebottleinriverraincancarrythepesticideintothewaterItalsocanentertheundergroundwaterSoil:sprypesticideEnterthepeople’sbodybyRespiratorytract：breathAlimentarycanal:drinkoreatfoodSkinChronictoxicityLongtermeffectCancerBirthdefectsInducetumorsEndocrinedisruptingchemicalDoYouKnowAllvegetablesandfruitsaresprayedwithtoxicsyntheticinsecticides&fungicidesofwhichsomearecarcinogenic(cancercausing).Allvegetables&fruitsfromfarmtothemarketplacemaygothroughhighlyunhygienichandlingtilltheyfinallyreachyou.(seed)Sowninthefarmer’sfieldpre-harvesttreatmentwithtoxicinsecticidestreatedwithtoxicinsecticides/fungicidesharvestedtradedinmarketplaceconsumerhomewaterwashed&consumedWhatisapesticideresidueOneormoresubstancespresentinoronplantsorproductsofplantorigin,edibleanimalproductsorelsewhereintheenvironmentandresultingfromtheuseofpesticide,includingtheirmetabolitesandproductsresultingfromtheirdegradationorreactionWhenacropistreatedwithapesticide,averysmallamountofthepesticide,orindeedwhatitchangestointhe

plant

(its'metabolites'or'degradationproducts'),canremaininthecropuntilafteritisharvested.Thisisknownasthe'residue'.

Pesticide

residuesmaybepresentin:

freshortinnedfruitandvegetablesprocessedfoodanddrink

madefromthecrop(e.g.juice,breadoranyothermanufacturedfoodordrink)freshorprocessedanimalproducts(iftheanimalshavebeenfedoncroptreatedwithpesticide).Itisimportant:toinsurequalityofagricultureproductsandtopreventpesticidepollution.achievedbyintensifyinginspectionandmonitoringsystem.instantdetectiontechniquesforpesticideresidue.MeasurementofthepesticideresidueObjectiveToqualitativeanalysistheresidueoforganophosphorousorcarbamatepesticidesinvegetables

Method

Calculatetheacetylcholinesteraseinhibitionrate

PrincipiumTheorganophosphorousorcarbamatepesticidescouldinhibittheactivitiesoftheacetylcholinesteraseTherewasapositiverelationshipbetweentheacetylcholinesteraseinhibitionrateandthepesticideconcentrationCont…MeasurerangeVegetablesleaf,fruit,bean,melonandsoonPesticidesOrganophosphorous:forexample,methamidophos,parathion,omethoate,dichlorvos,phorate,etc.Carbamates:forexample,carbaryl,methomyl,metolcarb,etc.

InstrumentsSP-723PVis-spectrophotometercultureboxelectronicbalancebeakerSalver：tweezers,shears,knifeandpenciltesttubeandtubeshelfPlasticsqueezebottleRubberpipettebulbPipette–sample,extractionsolutionMicrosampler：substrate,developer,acetylcholinesteraseReagentsThereagentpackageincludeacetylcholinesterase,substrate,developerandextractionsolutionAllofthewaterisdistilledwaterAllofthereagentsareanalyticalreagentinthisexperimentCont…1.extractionreagent:(phosphatebuffersolution)

Na2HPO4+KH2PO4;PH=8.0Dissolveonebaginto1000mldistilledwater2.specialenzyme:(acetylcholinesterase)powder,freezepreservedeverybottledissolvedinto8mldistilledwaterCont…3.substrate：（DithioAcetylcholineIodide

）dissolvedinto10mlextractionsolutionpreservedinthetemperatureof0~5℃4.developer:（DisulfideDinitroBenzoicacid）dissolvedinto10mlextractionsolutionpreservedinthetemperatureof0~5℃.Cont…SamplingrequirementLeafusingthesamplingequipmenttotaketheleavesfromthedifferentplantsatrandomFruittakingasamplefromthesurfacethicknessis1cm3mlextractionsolution

+50μlacetylcholinesterase,+50μldeveloper,culturingfor30minproductions+50μlsubstrate

Shakeupspilltocuvettemeasureimmediatelyyellowsolutiondifferenceofabsorbencyvalueoffore-and-aft3min.spectrophotometerActhecontrol3mlfiltratesolution+50μlacetylcholinesterase,+50μldeveloper,culturingfor30minproductions+50μlsubstrate

Shakeupspilltocuvettemeasureimmediatelyyellowsolutiondifferenceofabsorbencyvalueoffore-and-aft3min.spectrophotometerAsVegetablesample+20mlextractionsolutionandthenfiltratetheinhibatonrate>50%?Pesticideresidue?thesampleSampletreatment

inhibitoryreaction(add50μlacetylcholinesterase,3mlfiltrateand50μldevelopertothetesttube)Sampling(take2gramsample,or4gramifitisnotleafvegetable,andthencutup)Extracting(add20mlextractionsolution,shakefor1or2min,filtrate,andthenplacefor3min.)Culturing(putthesampletothecultureboxof37-38Cfor30min.)Color

reaction(takethesampleoutoftheculturebox,add50μlsubstrate,filltothecuvetteandmeasureon723Pspectrophotometerimmediately)

add50μlacetylcholinesterase,3mlextractionsolutionand50μldevelopertotesttubeCulturingputthecontroltothecultureboxof37-38Cfor30min.Color

reactiontakethesampleoutoftheculturebox,add50μlsubstrate,filltocuvetteandthenmeasureon723PspectrophotometerimmediatelyControl-sampletreatmentCalculatetheinhibitionrate

X:theinhibitionrateAc:thecontrol’sdifferenceofabsorbencyvalueoffore-and-aft3minAs:thesample’sdifferenceofabsorbencyvalueoffore-and-aft3minAnalysistheresulttheresultisexpressedasinhibitionrate(%)therate＜50％,free-pesticideresiduetherate≥50％,pesticideresidueexcessiveAttentionAc:0.4~0.8；ifthevalueofAcislessthan0.4,addmoreacetylcholinesterasepropriety.Afteraddingthesubstrate,colorimetricanalysisatonce.Themicrosampleroftheenzyme,chromogenicreagentandthesubstratewasforbiddentobeusedinconfusion.Cont….SP-732P

VisSpectrophotometerprinterparameterdisplayoperationpanelsampleroomswitchbetweenprinterandcomputer①.

DIGITALREADOUT.displaysthedataofTransmittance,Absorbance,Concentration.②③.WAVELENGTHSELECTOTKEYSis

usedtoselectthedesiredanalyticalwavelength,andusedtoselectthespecialfunctionsassettingtheparameterofstandardconcentration(concentrationvalueorfactor),lightsourceexchangeselection,LampON/OFF,etc.④FUNCTIONSELECTORselectsthefunctionsassettingtheparameterofstandardconcentration(concentrationvalueorfactor),lightsourceexchangeselection,LampON/OFF,etc.⑤ENTERKEYisusedtoenteringtheparameterofsampletothememoryorconfirmingthefunctionthatisselected.⑥100%T/0ACONTROLisusedtosetthedisplayto100%Tor0Abs.Itmustberesetwhenevertheanalyticalwavelengthhasbeenchanged.⑦M(jìn)ODESELECTORselectstheTransmittance,Absorbance,Concentrationmode.（tosetthemodeoftesting,choosethefirstmodewhichcanmeasure8samplesatthesametime,orthesecondmodewhichcanmeasure9samplesatthesametime.）⑧0%TCONTROL

isusedtosetthedisplayto0%Tbeforesetting0Abs.⑨CLEANKEYisusedforcleaningthewrongparameter.Sketchmapofthesamplecompartmentsamplesamplesamplesamplesamplesamplesamplesamplecontroladjust100%T12345678910operatingprocedure1.TurntheinstrumentonClosethesamplecompartmentcover,andturnonthepowerAllowtheinstrumenttoself-correctingandwarmupforatleast30minutes2.whentheself-correctingfinish,theinstrumententerthefollowinginterface3.pressthefunctionselectorkeytochoosethefunctionoffastmeasurementofpesticideresidue

功能

請(qǐng)輸入標(biāo)準(zhǔn)濃度

請(qǐng)輸入標(biāo)準(zhǔn)因子

請(qǐng)輸入波長(zhǎng)切換點(diǎn)

建立標(biāo)準(zhǔn)曲線。

農(nóng)藥殘毒快速檢測(cè)

調(diào)用標(biāo)準(zhǔn)曲線

刪除標(biāo)準(zhǔn)曲線functionpleaseinputthestandardconcentrationpleaseinputthestandardfactorspleaseinputthepointofwavelengthswitchdrawthestandardcurvefastmeasurementofthepesticideresiduetransferthestandardcurvedeletethestandardcurve農(nóng)藥殘毒快速檢測(cè)請(qǐng)放入標(biāo)樣及被測(cè)樣品開始檢測(cè)？正在檢測(cè)……

請(qǐng)稍后顯示檢測(cè)結(jié)果？顯示結(jié)