無(wú)機(jī)鹽緩沖液配方表

25°C下0.1mol/l磷酸鈉緩沖液的配制pH1mol/lNa2HPO4(ml)1mol/lNaH2PO4(ml)5.87.992.16.012.088.06.217.882.26.425.574.56.635.264.86.846.353.77.057.742.37.268.431.67.477.422.67.684.515.57.889.610.48.093.26.80.2mol/L磷酸鹽緩沖液(pH5.7?8.0)pH0.2mol/LNaH2PO4(ml)0.2mol/LNa2HPO4(ml)5.793.56.55.892.08.05.990.010.06.087.712.36.185.015.06.281.518.56.377.522.56.473.526.56.568.531.56.662.537.56.756.543.5

6.851.049.06.945.055.07.039.061.07.133.067.07.228.072.07.323.067.07.419.081.07.516.084.07.613.087.07.710.589.57.88.591.57.97.093.08.05.394.7NaH2PO4?2H2O：10.86105g(或NaHPO4?12H2O：21.8502g)+NaH2PO4?2H20:6.08595g(或NaH2PO4?H2O：10.76g),溶解，定容至1L上述藥品都是極易溶的，直接溶解就行了附磷酸氫二鈉-磷酸二氫鈉緩沖液(0.2mol/L)pH值；0.2mol/LNa2HPO4溶液（mL）；0.2mol/LNaH2PO4溶液（mL）5.8；8.0；92.05.9；10.0；90.06.0；12.3；87.76.1；15.0；85.06.2；18.5；81.56.3；22.5；77.56.4；26.5；73.56.5；31.5；68.56.6；37.5；62.56.7；43.5；56.5OTUlJ^OOhO^OCDCO7 6 67 6 62 7 —O O Ococo7 4OOCO7—7OO00Ooo2COOU15—Loo7.7"8951。.5700“915“357.9“93.5“7.58.0"94.7"5.3NaH2p04.2H20“Mrl78o5-0.2movL言*356g、廠NaHPO4A2H2o”MrH358.220.2movL胡商*71.64g、廠NaH2p04.2H20“Mrl56ow0.2movL言*31.21g、廠NaH2p04?H20“Mrl38or0.2movL胡商*27.6g戶。磷酸緩沖液(phosphatebuffer)按照下表所給定的體積，混合1mol/L的磷酸二氫鈉（單堿）和1mol/L磷酸氫二鈉（雙堿）貯液，獲得所需pH的磷酸緩沖液。配制1mol/L的磷酸二氫鈉（NaH2PO4"H2O）貯液：溶解138g于足量水中，使終體積為1L;1mol/L磷酸氫二鈉（Na2HPO4）貯液:溶解142g于足量水中使終體積為1L。1mol/L磷酸二氫鈉（ml）1mol/L磷酸氫二鈉（ml）最終pH值8771236.08501506.18151856.27752256.37352656.46853156.56253756.65654356.75104906.84505506.93906107.03306707.12807207.21mol/L磷酸二氫鈉（ml）1mol/L磷酸氫二鈉（ml）最終pH值8771236.08501506.18151856.27752256.37352656.46853156.56253756.65654356.75104906.84505506.93906107.03306707.12807207.2

Ph0.2mol/lNaH2PO4(ml)0.2mol/lNa2HPO4(ml)5.793.56.55.892.08.05.990.010.06.087.712.36.185.015.06.281.518.56.377.522.56.473.526.56.568.531.56.662.537.56.756.543.56.851.049.06.945.055.07.039.061.07.133.067.