建立基因敲除小鼠頸動(dòng)脈粥樣硬化模型英文

建立基因敲除小鼠頸動(dòng)脈粥樣硬化模型英文第1頁(yè)/共27頁(yè)Candidategenesinlipoproteinmetabolism

LusisetalCirculation.2004第2頁(yè)/共27頁(yè)LipoproteinLipaseintheArterialWall

Pentik?inenetalArteriosclerThrombVascBiol.2002第3頁(yè)/共27頁(yè)LipoproteinLipaseintheArterialWallPentik?inenetalArteriosclerThrombVascBiol.2002第4頁(yè)/共27頁(yè)HypothesisLPLArterialresponsetoinjuryhyperlipidemicconditions？第5頁(yè)/共27頁(yè)A1x2-mmstripoffilterpapersaturatedwith10%FeCl3solutionwasappliedtotheadventitialsurfaceofthesurgicallyexposedcarotidarteryfor3.0minutes,thenremoved.Thesurgicalincisionwassuturedwith4–0monofilament,andtheanimalwasreturnedtoitscage.第6頁(yè)/共27頁(yè)CommoncarotidarteriesofapoE-/-,LPL+/-(ELK)mice(A)andapoE-/-,LPL+/+(EK)mice(B)8weeksafterferricchlorideinjury第7頁(yè)/共27頁(yè)neointimaformationaftercarotidarteryinjurybyFeCl3for

8weeksELKcarotidarterystainedwithH&EEKnoninjuredinjured第8頁(yè)/共27頁(yè)neointimaformationaftercarotidarteryinjurybyFeCl3for

8weekscarotidarterystainedwithH&EELKEKnoninjuredinjured第9頁(yè)/共27頁(yè)MeanIntimalandMedialCross-sectionalAreasofEKandELKMice8WeeksafterInjuryEKELK***EKELKmedia2.95x104±20005.98x104±1000intimal4.01x104±60008.15x104±2000第10頁(yè)/共27頁(yè)lipiddepositaftercarotidarteryinjurybyFeCl3for

8weeksELKEKcontrolcarotidarterystainedwithOROnoninjuredinjured第11頁(yè)/共27頁(yè)lipiddepositaftercarotidarteryinjurybyFeCl3for

8weeksELKEKcarotidarterystainedwithOROnoninjuredinjured第12頁(yè)/共27頁(yè)lipiddepositaftercarotidarteryinjurybyFeCl3for

8weeksELKEKcarotidarterycounterstainedwithOROandhaematoxylininjurednoninjured第13頁(yè)/共27頁(yè)lipiddepositaftercarotidarteryinjurybyFeCl3for

8weeksELKEKcarotidarterycounterstainedwithOROandhaematoxylinnoninjuredinjured第14頁(yè)/共27頁(yè)Macrophagestainfor

8weeksELKEK第15頁(yè)/共27頁(yè)ELKEKVonWillebrandFactorStainfor

8weeks第16頁(yè)/共27頁(yè)0week4weeks8weeksELK（n=4）61.52±11.09113.9±5.98270.17±24.29EK（n=3）53.96±18.1761.61±3.1485.07±9.40Pvalue0.760.00150.0016****NS第17頁(yè)/共27頁(yè)0week4weeks8weeksELK(n=4)395.5±34.89718.7±40.52939.32±46.67EK（n=3）239.6±59.77530.7±59.52686.08±56.66Pvalue0.0970.040.018NS**第18頁(yè)/共27頁(yè)0week4weeks8weeksELK(n=4)118.3±7.9164.54±6.10109.7±4.46EK(n=3)85.19±17.7461.61±3.14109.53±2.90Pvalue0.190.680.98第19頁(yè)/共27頁(yè)0week4weeks8weeksELK17.75±0.7821.38±1.4521.75±2.18EK19.75±0.8822.63±0.7524.67±0.60Pvalue0.140.470.32NSNSNS第20頁(yè)/共27頁(yè)EKELKneointimaformationaftercarotidarteryinjurybyFeCl3for

4weekscarotidarterystainedwithH&E

第21頁(yè)/共27頁(yè)lipiddepositaftercarotidarteryinjurybyFeCl3for

4weeksELKEKcarotidarterycounterstainedwithOROandhaematoxylin第22頁(yè)/共27頁(yè)第23頁(yè)/共27頁(yè)ReferenceMaryBethDeYoung,CliffordTom,DavidA.Dichek,etalPlasminogenActivatorInhibitorType1IncreasesNeointimaFormationinBalloon-InjuredRatCarotidArteries.Circulation.2001;104:1972-1977KoichiKaikita,AgnesB.Fogo,LijunMa,JohnA.Schoenhard,NancyJ.Brown,DouglasE.Vaughan,etalPlasminogenActivatorInhibitor-1DeficiencyPreventsHypertensionandVascularFibrosisinResponsetoLong-termNitricOxideSynthaseInhibition.Circulation.2001;104:839-844YanhongZhu,PeterM.Farrehi,WilliamP.Fay,etalPlasminogenActivatorInhibitorType1EnhancesNeointimaFormationAfterOxidativeVascularInjuryinAtherosclerosis-ProneMice.Circulation.2001;103:3105-3110MariannePynn,KatrinSch?fer,StavrosKonstantinides,MartinHalle,etalExerciseTrainingReducesNeointimalGrowthandStabilizesVascularLesionsDevelopingAfterInjuryinApolipopr