模型2.細胞系腫瘤共236種均為人源可從庫http購買

（共236種，均為人源腫瘤細胞系，可從中 ） 6T-人T ,添SodiumPyruvate0.11g/L)，90NaHCO3 ,添SodiumPyruvate0.11g/L)，90NaHCO3AML- ，添加0.005mg/mlinsulin,0.005CCRF-CEM[CCRF ,添NaHCO3 ,添NaHCO3 ,添SodiumPyruvate0.11g/L)，90NaHCO3 ,添NaHCO3HL-IMDM培養(yǎng)基(GIBCO,貨號 HuT Jurkat,CloneE6- ,添加NaHCO31.5g/L,K-IMDM培養(yǎng)基(GIBCO,貨號 RPMI-1640（invitrogen11875）78mlFBS（Gibco）20mlGluta-max（invitrogen35050）1Sodium ）1 MEG- ,添加NaHCO31.5g/L,MOLT-胞 ,添加NaHCO31.5g/L,IMDM(Invitrogen,31980-030)90FBS(Gibco)10 ,添加NaHCO31.5g/L,SUP- THP- ,添加NaHCO31.5g/L,glucose2.5g/L, ,添加NaHCO31.5g/L,表皮細胞A-DMEM(Gibco,11960-044)88癌FBS(Gibco)10Glutamax(Gibco,35050-061)1SodiumPyruvate100mMSolution)1HCCC- ,添加NaHCO31.5g/L,glucoseSodiumPyruvate0.11g/L)，90%；胎牛，10%。解螺GBC- ,添加NaHCO395- ,添加NaHCO310%Calu-10%Calu-MEM培養(yǎng)基(GIBCO,貨 ，添加NaHCO3RPMI1640Medium(Invitrogen, )88mlFBS(Gibco)10mlGlutamax（ ）1SodiumPyruvate100mMSolution)1 ,添加NaHCO31.5g/L,glucose ,添加NaHCO3NCI-RPMI1640Medium(Invitrogen11875-093)88mlFBS(Gibco)10ml解螺旋 ）1SodiumPyruvate100mMSolution)1NCI-RPMI1640Medium(Invitrogen,11875-093)88FBS(Gibco)10mlinvitrogen35050）1SodiumPyruvate100mMSolution)1NCI-H1437RPMI1640Medium(Invitrogen, )88mlFBS(Gibco)10mlGlutamax（ ）1SodiumPyruvate100mMSolution)1NCI- ,添加NaHCO31.5g/L,RPMI1640Medium )88FBS(Gibco)10ml ）1SodiumPyruvate100mMSolution)1NCI-RPMI1640Medium(Invitrogen,11875-093)88mlFBS(Gibco)10mlGlutamax（invitrogen35050）1SodiumPyruvate100mMSolution)1NCI-RPMI1640Medium(Invitrogen, )88mlFBS(Gibco)10mlGlutamax（ ）1SodiumPyruvate100mMSolution)1NCI- ,添加NaHCO3glucoseNCI-RPMI1640Medium(Invitrogen11875-093)88mlFBS(Gibco)10ml invitrogen35050）1SodiumPyruvate100mMSolution)1NCI-RPMI1640Medium(Invitrogen,11875-093)88FBS(Gibco)10mlinvitrogen35050）1SodiumPyruvate100mMSolution)1NCI- ,添加NaHCO3glucoseNCI-RPMI1640Medium(Invitrogen,11875-093)88mlFBS(Gibco)10mlGlutamax（invitrogen35050）1SodiumPyruvate100mMSolution)1NCI-RPMI1640Medium(Invitrogen,11875-093)88FBS(Gibco)10mlinvitrogen35050）1SodiumPyruvate100mMSolution)1NCI-H292RPMI1640Medium(Invitrogen,11875-093)88FBS(Gibco)10mlGlutamax（ ）1mlSodiumPyruvate100mMSolution)1NCI-RPMI1640Medium(Invitrogen11875-093)88mlFBS(Gibco)10ml invitrogen35050）1SodiumPyruvate100mMSolution)1NCI-H446 ,添加NaHCO3glucoseNCI-H460 ,添加NaHCO3glucoseNCI- ,添加NaHCO3glucoseNCI-H838RPMI1640Medium(Invitrogen,11875-093)88FBS(Gibco)10mlGlutamax（invitrogen35050）1mlSodiumPyruvate100mMSolution)1SH-ACL4+5%SH-ACL4+5%SH-ACL4+5%SH-ACL4+5%SH-HITES+5%SH-ACL4+5%FBSMEM(Invitrogen， )87mlFBS(Gibco)10mlGlutamax（ ）1mlNon-essentialAminoAcids,100X )1SodiumPyruvate100mM ）1MEM培養(yǎng)基(GIBCO,貨 ，添加NaHCO3 ,添加NaHCO31.5g/L,glucoseBEL- ,添加NaHCO31.5g/L,glucoseBEL- ,添加NaHCO31.5g/L,glucoseHep3B2.1-7[HepHep-3B,MEM(Invitrogen， )87mlFBS(Gibco)10mlGlutamax（ ）1mlNon-essentialAminoAcids,100′ )1SodiumPyruvate100mM ）1HepMEM(Invitrogen， )87mlFBS(Gibco)10mlGlutamax（ ）1mlNon-essentialAminoAcids,100x )1SodiumPyruvate100mM ）1HuH-DMEM培養(yǎng)基(GIBCO,貨號 ，添加NaHCO31.5g/L)，90%；優(yōu)質(zhì)胎牛血HuH-DMEM(Invitrogen,11960-044)88mlFBS(Gibco)10mlGlutamax(Invitrogen,35050-061)Non-essentialAminoAcids,100X(Invitrogen,11140-050)Li- ,添加NaHCO31.5g/L,glucoseMEM培養(yǎng)基(GIBCO,貨 ，添加NaHCO31.5g/L，Sodium ,添加NaHCO31.5g/L,glucoseQGY- ,添加NaHCO31.5g/L,glucoseMEM培養(yǎng)基(GIBCO,貨 ，添加NaHCO3SMMC- ,添加NaHCO31.5g/L,glucoseSNU-RPMI1640Medium(Invitrogen,11875-093)88mlFBS(Gibco)10mlGlutamax（ ）1SodiumPyruvate100mMSolution )1SNU-RPMI1640Medium(Invitrogen,11875-093)88mlFBS(Gibco)10mlGlutamax（ ）1SodiumPyruvate100mMSolution )1 ,添加NaHCO31.5g/L,glucoseC-33 ，添加NaHCO3Ca ,添加NaHCO31.5g/L,glucoseHCC94 ,添加NaHCO31.5g/L,glucose2.5g/L,SodiumPyruvate0.11g/L)，90%；胎牛，10%。MEM培養(yǎng)基(GIBCO,貨 ，添加NaHCO3HeLaMEM培養(yǎng)基(GIBCO,貨號 ，添加NaHCO31.5g/L，SodiumPyruvate0.11g/L)，90%；胎牛，10%?；騌PMI-1640(GIBCO,貨號 ,添加NaHCO31.5g/L,glucose2.5g/L,SodiumPyruvate0.11g/L)，90%；胎牛血ME-10%EagleMEM(Invitrogen, )88mlFBS(Gibco)10mlGlutamax（ ）1mlNon-essentialAminoAcids,100X )1MEM培養(yǎng)基(GIBCO,貨 ，添加NaHCO3MEM培養(yǎng)基(GIBCO,貨 ，添加NaHCO3MG-MEM培養(yǎng)基(GIBCO,貨 ，添加NaHCO31.5g/L，SodiumMNNG/HOSCl#51059-MEM培養(yǎng)基(GIBCO,貨 ，添加NaHCO3U-2McCoy's5aMedium(Invitrogen,12330-031)90FBS(Gibco)10RPMI1640（invitrogen11875）FBS(Gibco)10Gluta-max（invitrogen35050）1Sodiumpyruvate ）1A-375DMEM(Invitrogen,12430-054)89FBS(Gibco)10SodiumPyruvate(Invitrogen,11360-070)1ml（1 10%橫紋肌肉A- 瘤HEp- ,添加NaHCO31.5g/L,glucose滑膜肉瘤SW982[SW-982, HCT-8[HRT- ,添加NaHCO31.5g/L,glucose ,添加NaHCO31.5g/L,glucoseRPMIMedium1640(Invitrogen,11875-093)95FBS(Gibco)5B-RPMIMedium1640(Invitrogen,11875-093)87FBS(Gibco)10ml解螺 NEAA)1mlGlutamax（invitrogen ）1mlSodiumPyruvate100mMSolution(invitrogen )1mlDMEM(Invitrogen,12430)80FBS(Gibco)20RPMI-1640 )90FBS(Gibco)10CAL-DMEM(Invitrogen,12430)90FBS(Gibco)10HTh-DMEM(Invitrogen,12430)90FBS(Gibco)10RPMI-1640 )90FBS(Gibco)10KHM-RPMI-1640 )90FBS(Gibco)10KMH-DMEM(Invitrogen,12430-054)45RPMIMedium1640(Invitrogen,11875-093)45FBS(Gibco)10KTC-RPMIMedium1640(Invitrogen,11875-093)87mlFBS(Gibco)10mlNEAA )1 ）1SodiumPyruvate100mMSolution )1F12K培養(yǎng)基(SIGMA,貨號N3520，添加NaHCO32.5g/L)，90%；優(yōu)質(zhì)胎牛10%DMEM(Invitrogen,12430)95FBS(Gibco)5RPMIMedium1640(Invitrogen,11875-093)95FBS(Gibco)5SW579[SW579;SW- NCI-H2452 ,添加NaHCO31.5g/L,glucose SHG- ,添加NaHCO31.5g/L,glucoseU-118DMEM培養(yǎng)基(GIBCO,貨號 ，添加NaHCO31.5g/L)，90%；優(yōu)質(zhì)胎牛血 10%U-87MEM培養(yǎng)基(GIBCO,貨 ，添加NaHCO3 ,添加NaHCO31.5g/L,glucoseCOLO ,添加NaHCO31.5g/L,glucose ,添加NaHCO31.5g/L,glucose2.5g/L, 0.11g/L)，90%；胎牛血清，10%。或DMEM培養(yǎng)基(GIBCO,貨號 10%CW- ,添加NaHCO31.5g/L,glucose2.5g/L, 0.11g/L)，90%；胎牛血清，10%?；駾MEM培養(yǎng)基(GIBCO,貨號 HCTHT-McCoy's5aMedium(Invitrogen,12330-031)90FBS(Gibco)1010%LSDMEM培養(yǎng)基(GIBCO,貨號 ，添加NaHCO31.5g/L)，90%；優(yōu)質(zhì)胎牛血清，10%。解螺旋MEM培養(yǎng)基(GIBCO,貨 ，添加NaHCO3MEM培養(yǎng)基(GIBCO,貨 ，添加NaHCO3RKO-MEM培養(yǎng)基(GIBCO,貨 ，添加NaHCO3 SW480[SW-L-15Hyclone,SH30525.01Invitrogen11415-064)90FBS(Gibco)10 SW-L-15(Hyclone,SH30525.01)90FBS(Gibco)10 MEM(Invitrogen， )77mlFBS(Gibco)20mlGlutamax（ ）1mlNon-essentialAminoAcids,100X )1SodiumPyruvate100mM ）1DLD- ,添加NaHCO31.5g/L,glucoseHCT- ,添加NaHCO31.5g/L,glucoseHam'sF-12K(Kaighn's)Medium(Invitrogen,21127-022)90FBS(Gibco)10 ,添加NaHCO31.5g/L,glucoseNCI-H716 ,添加NaHCO31.5g/L,glucoseMEM培養(yǎng)基(GIBCO,貨號 ，添加NaHCO31.5g/L，SodiumPyruvate0.11g/L)，90%；胎牛，10%?；騌PMI-1640(GIBCO,貨 ,添NaHCO31.5g/L,glucose2.5g/L,SodiumPyruvate0.11g/L)，90%；胎牛血BC- ）78mlFBS（Gibco）20ml ）1Sodium ）1 ,添加NaHCO31.5g/L,glucoseJeKo- ,添加NaHCO31.5g/L,glucose ,添加NaHCO31.5g/L,glucoseNK- ，添加NaHCO31.5g/L，肌醇43.2mg/L,葉酸8.82mg/L,100-200單位/ml重組IL-2,β-巰基乙醇7.8mg/L)，90%；優(yōu) ,添加NaHCO31.5g/L,glucoseU- ,添加NaHCO31.5g/L,glucose癌Caov-3DMEM(Invitrogen,12430-054)89mlFBS(Gibco)10mlSodiumPyruvate100mM ）1癌10%癌HO- ,添加NaHCO31.5g/L,glucose癌HO- ,添加NaHCO31.5g/L,glucose癌RPMI-1640(GIBCO,貨號,添加NaHCO31.5g/L,glucose2.5g/L,癌10% ,添加NaHCO31.5g/L,glucose癌 ,添加NaHCO31.5g/L,glucose癌MEM培養(yǎng)基(GIBCO,貨 ，添加NaHCO3癌 McCOY's5A(SIGMA,貨號M4892，添加NaHCO32.2g/L)，90%；優(yōu)質(zhì)胎牛10%癌 ,添加NaHCO31.5g/L,glucose癌SW780[SW-780, 癌McCoy's5aMedium(Invitrogen,12330-031)90FBS(Gibco)10癌EMEM(Invitrogen， )87mlFBS(Gibco)10mlGlutamax（ ）1mlNon-essentialAminoAcids,100x )1SodiumPyruvate100mM ）1癌MEM培養(yǎng)基(GIBCO,貨 ，添加NaHCO3胚胎橫紋 10%癌RPMIMedium1640(Invitrogen,11875-093)88mlFBS(Gibco)10mlGlutamax（ ）1Sodium ）1癌MEM(Invitrogen， )87mlFBS(Gibco)10mlGlutamax（ ）1mlNon-essentialAminoAcids,100′ )1SodiumPyruvate100mM ）1癌LNCaPCloneRPMIMedium1640(Invitrogen,11875-093)88FBS(Gibco)10mlGlutamax（invitrogen35050）1mlSodium ）1癌LNCaPclone ,添加NaHCO31.5g/L,glucose癌PC-F-12培養(yǎng)基(GIBCO,貨號 ，添加L-glutamine300mg/L,NaHCO3癌 ,添加NaHCO31.5g/L,glucoseJEG-Bcap- ,添加NaHCO31.5g/L,glucoseBT-474 ,添加NaHCO31.5g/L,glucoseSodiumPyruvate0.11g/L90FBS10BT-RPMI-1640(GIBCO,貨號,添加NaHCO31.5g/L,glucose2.5g/L,糖DMEM+20%優(yōu)質(zhì)胎牛。CCD-MEM培養(yǎng)基(GIBCO,貨 ，添加NaHCO3 ,添加NaHCO31.5g/L,glucoseHs MEM )88FBS(Gibco)10ml ）1Non-essentialAminoAcids,100X(Invitrogen, 1mlhumanbinantinsulin（SIGMA,91077C-1G）終濃度0.01mg/mlMCF7[MCF-MEM培養(yǎng)基(GIBCO,貨號 ，添加NaHCO31.5g/L，SodiumPyruvate0.11g/L，0.01mg/mlbovineinsulin)，90%；優(yōu)質(zhì)胎牛，10%。L-15Hyclone,SH30525.01Invitrogen11415-064)90FBS(Gibco)10 L-15培養(yǎng)基(GIBCO,貨號 )添加10ug/ml胰和10ug/ml谷胱甘85%；優(yōu)質(zhì)胎牛，15%。解螺 L-15Hyclone,SH30525.01Invitrogen11415-064)90FBS(Gibco)10 T-DMEM培養(yǎng)基(GIBCO,貨 ，添加NaHCO31.5g/L)，90%；優(yōu)質(zhì)胎牛 ,添加NaHCO31.5g/L,glucose ,添加NaHCO31.5g/L,glucose軟骨肉瘤細SW Tca- ,添加NaHCO31.5g/L,glucose神經(jīng)母細IMR-MEM培養(yǎng)基(GIBCO,貨 ，添加NaHCO3神經(jīng)母細SH- ）F12(invitrogen )43.5mlFBS(Gibco)10ml ）1Sodium ）1NEAA )1神經(jīng)母細SH-MEMGIBCO,貨號，添加NaHCO31.5g/L，SodiumPyruvate0.11g/L)，45%；F-12GIBCO,貨號，添加L-glutamine300mg/L,NaHCO31.5g/L)，45%；優(yōu)質(zhì)胎牛，10%。神經(jīng)母細SK-N-MEMGIBCO,貨號，添加NaHCO31.5g/L，SodiumPyruvate0.11g/L)，45%；F-12GIBCO,貨號，添加L-glutamine300mg/L,NaHCO31.5g/L)，45%；優(yōu)質(zhì)胎牛，10%。神經(jīng)母細SK-N- ）87mlFBS（Gibco）10ml ）1NEAA )1Sodium ）1神經(jīng)母細SK-N-MEM培養(yǎng)基(GIBCO,貨 ，添加NaHCO3神經(jīng)上皮瘤MEM培養(yǎng)基(GIBCO,貨 ，添加NaHCO31.5g/L，Sodium769- ,添加NaHCO31.5g/L,glucose786-O786-0 ,添加NaHCO31.5g/L,glucoseMEM培養(yǎng)基(GIBCO,貨 ，添加NaHCO3Caki-胞 ,添加NaHCO31.5g/L,glucose腎母細胞瘤McCOY's5A(SIGMA,貨號M4892，添加NaHCO32.2g/L)，85%；優(yōu)質(zhì)胎牛15%95%；二氧化碳，5%KP-N- ,添加NaHCO31.5g/L,glucoseSW- Eca- ,添加NaHCO31.5g/L,glucoseKYSE-RPMI1640/F12,10TE- ,添加NaHCO31.5g/L,glucose ,添加NaHCO31.5g/L,glucose ,添加NaHCO31.5g/L,glucose髓母細胞MEM(Invitrogen， )87mlFBS(Gibco)10mlGlutamax（ ）1mlNon-essentialAminoAcids,100X )1SodiumPyruvate100mM ）1 ，添加NaHCO3F12K培養(yǎng)基(SIGMA,貨號N3520，添加NaHCO32.5g/L)，90%；優(yōu)質(zhì)胎牛BGC- ,添加NaHCO31.5g/L,glucoseHGC- ,添加NaHCO31.5g/L,glucoseKATO FBS(Gibco)20 ,添加NaHCO31.5g/L,glucoseNCI-RPMI1640Medium(Invitrogen,11875-093)88mlFBS(Gibco)10mlGlutamax（invitrogen35050）1