PFC腦區(qū)促進(jìn)睡眠神經(jīng)元

CorrespondingAuthorYangDan,PhDBS,physics,BeijingUniversityPhD,neurobiology,ColumbiaUniversityCurrentpositionsHowardHughesMedicalInstituteUniversityofCalifornia,Berkeley

CurrentprojectsNeuralcircuitscontrollingsleep.Functionoftheprefrontalcortex(PFC).目前一頁(yè)\總數(shù)二十一頁(yè)\編于十五點(diǎn)BackgroundDifferentwavesgalleryhip/rem-sleep-cycle.html目前二頁(yè)\總數(shù)二十一頁(yè)\編于十五點(diǎn)BackgroundSleepcyclegalleryhip/rem-sleep-cycle.html目前三頁(yè)\總數(shù)二十一頁(yè)\編于十五點(diǎn)BackgroundPOA

Lesions

inthePOAcausesleepimpairment.Sleep-activeneuronsexistinthePOA.Thesleep-activeneuronsarespatiallyintermingled

withwake-activeneurons.Preopticarea,aregionofthehypothalamusmedialpreopticnucleusmedianpreopticnucleuslateralpreopticnucleusperiventricularpreopticnucleus/wiki/Preoptic_area目前四頁(yè)\總數(shù)二十一頁(yè)\編于十五點(diǎn)BackgroundTMNTuberomammillarynucleus,aregionoftheposteriorhypothalamuslargely,wake-promotinghistaminergicneuronsarousal,learning,memory,sleepandenergybalance./wiki/Tuberomammillary_nucleus目前五頁(yè)\總數(shù)二十一頁(yè)\編于十五點(diǎn)GABAPOA→TMNExtendedDataFigure1a目前六頁(yè)\總數(shù)二十一頁(yè)\編于十五點(diǎn)GABAPOA→TMNGABAergicneuronshistaminergicneuronsPOATMNGABAPOA→TMNExtendedDataFigure1b目前七頁(yè)\總數(shù)二十一頁(yè)\編于十五點(diǎn)GABAPOA→TMN

singlesoutthesleep-promotingneuronsGABAPOA→TMN96.2±1.4%95.4±3.7%ExtendedDataFigure2h目前八頁(yè)\總數(shù)二十一頁(yè)\編于十五點(diǎn)GABAPOA→TMN

singlesoutthesleep-promotingneuronsGABAPOA→TMN73.6±4.8%SleepreboundGABAergicneuronshistaminergicneuronsPOATMNGABAPOA→TMNSleep-promotingExtendedDataFigure2i目前九頁(yè)\總數(shù)二十一頁(yè)\編于十五點(diǎn)TwopartsoftheessayIdentificationofpreopticsleepneuronsusingretrogradelabellingandgeneprofiling目前十頁(yè)\總數(shù)二十一頁(yè)\編于十五點(diǎn)RetrogradelabellingCausalrole:OptogeneticactivationorinhibitionofGABAPOA→TMNneuronsenhancesorsuppressessleep.Figure1Extendeddatafigure3目前十一頁(yè)\總數(shù)二十一頁(yè)\編于十五點(diǎn)RetrogradelabellingDataanalysisActivationInhibitionControlExtendeddatafigure5目前十二頁(yè)\總數(shù)二十一頁(yè)\編于十五點(diǎn)RetrogradelabellingFunctionallydistinct:OptogeneticactivationofGABAPOAorVGLUTPOA→TMNneuronspromoteswakefulness.Figure3KEY1:TMNprojectingKEY2:GABAergic目前十三頁(yè)\總數(shù)二十一頁(yè)\編于十五點(diǎn)RetrogradelabellingOptrode:MeasuredirectlythespikingactivityofChR2-labelledGABAPOA→TMNneurons.Figure4目前十四頁(yè)\總數(shù)二十一頁(yè)\編于十五點(diǎn)RetrogradelabellingOptrode:MeasuredirectlythespikingactivityofChR2-labelledGABAPOA→TMNneurons.17identifiedGABAPOA→TMNneurons51unidentifiedPOAunitsFigure3目前十五頁(yè)\總數(shù)二十一頁(yè)\編于十五點(diǎn)GeneprofilingTRAPTranslatingRibosomeAffinityPurificationFigure4aRNA-seq目前十六頁(yè)\總數(shù)二十一頁(yè)\編于十五點(diǎn)GeneprofilingCCKCRHFigure4bcTRAP(cholecystokinin)(corticotropin-releasinghormone)目前十七頁(yè)\總數(shù)二十一頁(yè)\編于十五點(diǎn)GeneprofilingCCKCRHExtendeddatafigure10目前十八頁(yè)\總數(shù)二十一頁(yè)\編于十五點(diǎn)GeneprofilingRNA-seqTAC1(Preprotachy-kinin-1)&PDYN(ProDynorpy-in)目前十九頁(yè)\總數(shù)二十一頁(yè)\編于十五點(diǎn)ConclusionUsingoptogeneticmanipulationandrecording,virus-mediatedcircuittracing,andgeneprofiling,wehavecharacterizedapopulationofPOAsleepneurons