LncRNA全國(guó)講座-課件

LncRNA全國(guó)講座“Learner&Nontrivial&Confidence”-RNA全國(guó)技術(shù)中心技術(shù)LncRNA全國(guó)講座“Youstartprojectsandyouhavenoideawhattoexpect.It’sthatkindofchallengethatIenjoyverymuchasascientist.”LncRNA全國(guó)講座CircRes2012LncRNA全國(guó)講座TherapeuticapproachInclinicaltrialsApprovedtherapy

EnzymereplacementtherapyYESYESGenetherapyYESYESNuclearreceptorligandsYESYESChromatinorDNAmodulatorsYESYESArtificialtranscriptionfactors YESNOMicroRNAmodulators YESNOCis-actinglncRNAmodulators NONOTrans-actinglncRNAmodulators NONO非タンパクコードRNAを利用した醫(yī)薬開(kāi)発GenomeNetworkProtein2Protein1Protein3Protein4Protein5Protein5PGene2ProteinXGene10ncRNA3ncRNA4Gene5Gene6DrugGene7Protein10Protein12Gene3Protein7Protein7Protein8Protein9Gene1Protein11Protein13ncRNA1ncRNA6Gene4Gene11Gene9ncRNA2Gene8DiseaseNewTargetofMedicineMutsumiKanamori-KatayamaPh.D.YoshihideHayashizakiM.D.,Ph.D.RIKENGenomeSciencesCenterGenomeExplorationResearchGroupLncRNA全國(guó)講座LncRNA全國(guó)講座LncRNA全國(guó)講座Ryanet.al,BioEssays29:288–299(2007)LncRNA>200nt結(jié)構(gòu)類似mRNA具有外顯子/內(nèi)含子低蛋白編碼潛力LncRNA全國(guó)講座LncRNA全國(guó)講座LncRNA全國(guó)講座CabiliMN.IntegrativeannotationofhumanlargeintergenicnoncodingRNAsrevealsglobalpropertiesandspecificsubclasses.GenesDev.2011Sep15;25(18):1915-27LncRNA全國(guó)講座LncRNA全國(guó)講座LncRNA全國(guó)講座LncRNA全國(guó)講座ncRNAPANDA募集轉(zhuǎn)錄因子NF-YA，啟動(dòng)細(xì)胞凋亡通路LncRNA全國(guó)講座Cell2011ncRNA-andPc2Methylation-DependentGeneRelocationbetweenNuclearStructuresMediatesGeneActivationProgramsLncRNA全國(guó)講座NatureMedicine2010ExpressionofanoncodingRNAiselevatedinAlzheimer’sdiseaseanddrivesrapidfeed-forwardregulationofβ-secretaseexpression早發(fā)老年性癡呆病中BACE1-AS高表達(dá)導(dǎo)致BACE1mRNA穩(wěn)定性增高，蛋白積聚，體現(xiàn)了ncRNA的毒性效應(yīng)BACE1BACE1-ASLncRNA全國(guó)講座拷貝數(shù)染色體定位表達(dá)LncRNA全國(guó)講座LncRNA全國(guó)講座IF:31.027LncRNA全國(guó)講座LncRNA全國(guó)講座LncRNA全國(guó)講座芯片結(jié)果聚類圖測(cè)序結(jié)果散點(diǎn)圖backNorthernblotlncRNA-DC在免疫細(xì)胞中的表達(dá)情況lncRNA-DC在DC/mono細(xì)胞群中的表達(dá)情況backChIP-Seq與ChIP-PCR結(jié)果back熒光素酶報(bào)告分析，驗(yàn)證轉(zhuǎn)錄因子結(jié)合區(qū)域LncRNA全國(guó)講座backLncRNA全國(guó)講座干擾結(jié)果過(guò)表達(dá)結(jié)果backLncRNA全國(guó)講座抑制DC細(xì)胞分化back通過(guò)STAT3的RIP實(shí)驗(yàn)驗(yàn)證LncRNA全國(guó)講座Lnc-DC與STAT3相互結(jié)合back進(jìn)一步驗(yàn)證Pulldown和RIP的結(jié)果LncRNA全國(guó)講座back結(jié)果表明，lnc-DC的3′末端序列（265-417）與STAT3的C端結(jié)合（583-770）。STAT3的活化依賴C端Tyr705的磷酸化。LncRNA全國(guó)講座back研究結(jié)果表明，S3I-201處理的細(xì)胞系與RNAi干擾后的細(xì)胞系在基因表達(dá)差異方面非常相似。LncRNA全國(guó)講座LncRNA全國(guó)講座高通量檢測(cè)生物信息分析表達(dá)、定位驗(yàn)證上游驗(yàn)證功能驗(yàn)證下游驗(yàn)證LncRNA全國(guó)講座LncRNA全國(guó)講座LncRNA全國(guó)講座LncRNA全國(guó)講座Affymetrix?TranscriptomeAnalysisConsole2.0(TAC2.0)Software

LncRNA全國(guó)講座LncRNA全國(guó)講座LncRNA全國(guó)講座IF:10.143LncRNA全國(guó)講座IF:4IF:11.139LncRNA全國(guó)講座LncRNA全國(guó)講座上游下游反義鏈正義鏈LncRNA全國(guó)講座LncRNA全國(guó)講座LncRNA全國(guó)講座

Hepatology2011LncRNA全國(guó)講座通過(guò)網(wǎng)絡(luò)找到調(diào)控lncRNA最多的轉(zhuǎn)錄因子同時(shí)找到能被不同轉(zhuǎn)錄因子調(diào)控最多的lncRNA使用德國(guó)Biobase數(shù)據(jù)庫(kù)商業(yè)版數(shù)據(jù)庫(kù)LncRNA全國(guó)講座LncRNA全國(guó)講座AffymetrixPromoter1.0RArrayAffymetrixHTA2.0LncRNA全國(guó)講座藍(lán)色線表示兩點(diǎn)之間的關(guān)系需要計(jì)算綠色線表示兩點(diǎn)之間的關(guān)系通過(guò)轉(zhuǎn)錄因子的預(yù)測(cè)得到LncRNA全國(guó)講座LncRNA全國(guó)講座IF:38.597LncRNA全國(guó)講座深灰色：激活淺灰色：抑制LncRNA全國(guó)講座I