甘藍(lán)型油菜低溫誘導(dǎo)的轉(zhuǎn)錄組和蛋白組分析

importantoilseedcrop,theBrassicanapusisofgreatsignificanceforstabilizingrapeseedyield.Inrecentyears,withthedevelopmentofomicsbecomeimportantmeanstorevealtheresponsemechanismsofBrassicanapusastheresearchtechniques,aimingtocomprehensivelyanaresponsemechanismofBrassicanapusunderlow-temperatureofcoldtoleranceinrapeseed.Inthisstudy,wefirsaftertreatment.Subsequently,acomprehensiveanalysisofthetranscriptomeandproteomeofBrassicanapusunderlowtemperaturestresswassequencingandmassspectrometrytechniquelevelsofBrassicanapusufurtherexplorethefunctionsandmechanismsofthesekeymoleculesinthecoldtoleranceofBrassicannewideasandmethodsforgeneticThisarticleaimstocomprehensivelyreveresponsemechanismofBrassicanapusunderlowtemperatstressthroughtranscriptomeandproteimprovementofcoldtoleranceinrapeseed.二、材料與方法selectedBrassicanapusL.astheexperimentalmaterienvironment,wellgrowintoinductiontreaSamplesweretakensubsequenttranscriptomeandthereferencegenomeusingCufflinksoftware.PerformProteinextractionandmassseparatedbySDSelectrophoresis.SelectproteinbandsformassspectrometryanalysistooProteomicdataanalysis:ComparetheproteinseobtainedfrommassspectrometryanalysiswofBrassicanapusproteinstodeterminetheiridentity.UseRBioinformaticsanalysis:Combiningtranscriptomeaproteomicdata,conductin-depthanalysisonthemolecularresponsemechanismofBrassicanapusunderlowtemperaturestress.Usingbioinformatgenesandproteins,inordertorevealtheirinvolvementintranscriptomeandproteomedata,thisstudyselectWesternBlotresultswithRNAseqandanalyzedthetranscriptomeandproteomechangesofBnapusunderlowtemperaturestress,providingstrongsupportforadeeperunderstandandinducedbiologicalreactionsthroughlow-temptreatment.Subsequently,transcriptometechnologyandmassspectrometryanalysis,wehofgenesthatundergosignificantchangesundbiologicalfunctions,includingsignaltransmetabolicprocesses,transcriptionalregulation,etc.Amonginthelow-temperatureadapexpressionlevelsofmanyptreatment.Theseproteinsinvolvevarisuchasenergymetabolism,proteinsynthesis,cellimportanceoftheseproteinsinthelow-tempmechanismofBrassicanapus.expressionpatternsandabundancechangesundlow-temperatureinduction.ThesegeneofBrassicanapus,whichisthefocusofoursubsequentthroughtranscriptomeandproteomicanalysis.Theseresultsreferencesforthecoldanalyzeditstranscriptomeandproteomettranscriptomeanalysis,weidentifiedaseriesofdifferentiallyexpressedgenestress,whichmayplayanimportantroleintheresistanceofC-repeat/DRE順式作用元件上，進(jìn)而激活一系列下游冷響應(yīng)基因的crucialregulatoryroleinplantresistancetolowtemperaturestress.TheCBF/DREB1transcripexpressionofaseriesofdownstreamcoldresponsivegenes.deeperunderstandingofthelow-temperaturestressresponsemechanisminBrassicanapus.resistanceofBrassicanapustolowtemperaturestress.Antioxidantenzymescanelreduceoxidativedamage;Iontransportproteinscionbalancebothinsideandimbalancedosmoticpressure;EproteinscanprovidesufficientenergysunormalgrowthanddevelopmentofBraWealsodiscoveredsomeunknowngenesandresourcesforBrassicanapustoresistlowtemperaturestreswhichisofgreatsignificanceforfurtherrresponsemechanismofBrassicanapustolowtemperaturestress.ThisstudyrevealedpartistressresponseinBrassicanapusunderstandingofthemolecularmechanismsofBrassicanapusinresistinglowtemprevealthemolecularnetworkoflowtemperaturestressresponsecomplexmechanismsoflowtempBrassicanapusbyconstructinggeneinteractionnetwortechnicalsupportforcoldresistantbreedingandproductionmanagementofBrassicanapusunderlowtresultsindicatethatthetranscriBrassicanapusundergosignificantchangesunderlowtemperaturestress,involvmechanismofBrassicanapusunderlowtemperaturestress.fdifferentiallyexpreunderstandthemolecularregulatorynetworkofBrassicanapusunderlowtemperatuproteinexpressionprofilesbeforeandafterlowtemperaturetreatment.Theseproteincellstructuremaintenance,indexpressionofthesekeyproteinsunderlowtemperatureBasedontheanalysisfoundthatthemolecularresponsemechanismofBrassicanapusunderstandingofthecoldtbutalsoprovidesatheoreticalbasisandcandidatresourcesforimprovingthe性具有重要的理論和實踐意義。ThisstudyrevealedthemoleculinformationandidtoleranceofBrassicanapus.Theseresultshaveimportantmolecularmechanismsofplantcoldtoleranceandimprovingthecoldtoleranceofrapeseed.在完成這篇《甘藍(lán)型油菜低溫誘導(dǎo)