[畢業(yè)設(shè)計(jì)精品] 基于微型光譜儀對(duì)水體中氨氮的實(shí)時(shí)在線檢測(cè).doc

本科學(xué)生畢業(yè)設(shè)計(jì)（論文）基于微型光譜儀對(duì)水體中氨氮的實(shí)時(shí)在線檢測(cè)學(xué)生：學(xué)號(hào)：指導(dǎo)教師：教授專業(yè)：制藥工程化學(xué)化工學(xué)院二O一一年六月本科學(xué)生畢業(yè)設(shè)計(jì)（論文）摘要I摘要環(huán)境保護(hù)逐漸成為當(dāng)今社會(huì)的一個(gè)焦點(diǎn)問題，水質(zhì)的檢測(cè)又是環(huán)境保護(hù)的一個(gè)重要組成部分。需樣量大，費(fèi)時(shí)，操作繁瑣是傳統(tǒng)的水質(zhì)監(jiān)測(cè)技術(shù)的突出不足，不便于進(jìn)行水質(zhì)的在線監(jiān)測(cè)。微型光譜儀在線檢測(cè)技術(shù)是新興的技術(shù)，具有方便，快捷，高效，樣品用量少等特點(diǎn)，可以實(shí)現(xiàn)水中多種污染物的快速檢測(cè)，是未來化學(xué)分析技術(shù)的一種重要方法，具有非常廣闊的發(fā)展空間。本文利用分光光度法快速檢測(cè)水中氨氮含量，并實(shí)現(xiàn)水質(zhì)的實(shí)時(shí)、在線檢測(cè)。在研究水楊酸分光光度法(GB/T8538-1995)的基礎(chǔ)上，通過使用二氯乙腈尿酸鈉代替標(biāo)準(zhǔn)中所用的次氯酸鈉，使用檸檬酸三鈉作為掩蔽劑，調(diào)節(jié)體系PH三個(gè)方面的研究探討，對(duì)上述因素在氨氮含量檢測(cè)中的影響進(jìn)行表征和優(yōu)化，得到這些影響因素的最優(yōu)值，探索出一套以紫外可見分光光度計(jì)為檢測(cè)手段，在10min內(nèi)得到水樣中氨氮含量結(jié)果的檢測(cè)方法。對(duì)實(shí)驗(yàn)條件進(jìn)行優(yōu)化后，運(yùn)用此方法將氨氮的標(biāo)準(zhǔn)樣品在自主研發(fā)的基于MS-II微型光譜儀的多參數(shù)水質(zhì)監(jiān)測(cè)儀上進(jìn)行檢測(cè)，作出標(biāo)準(zhǔn)曲線。多參數(shù)水質(zhì)監(jiān)測(cè)儀檢測(cè)模擬水樣結(jié)果與島津UV2450紫外分光光度計(jì)檢測(cè)結(jié)果進(jìn)行對(duì)比，結(jié)果顯示，多參數(shù)水質(zhì)監(jiān)測(cè)儀的檢測(cè)結(jié)果和島津UV2450基本一致，誤差小于5%選用二氯乙腈尿酸鈉代替次氯酸鈉，二氯乙腈尿酸鈉的體系濃度為0.313mmol/L，加入量為0.2ml。反應(yīng)溫度維持在30（0.5），用氫氧化鈉調(diào)節(jié)體系PH值為13左右。反應(yīng)時(shí)間為10min。在上述最佳檢測(cè)條件下，運(yùn)用島津UV2450紫外分光光度儀，得出氨氮含量在0-0.5mg/L濃度范圍內(nèi)的標(biāo)準(zhǔn)曲線為y=0.4423x-0.0163，R2=0.9984，檢測(cè)靈敏度為0.4423，回收率為99.49%，RSD為1.24%,最低檢出限為0.0087mg/L。測(cè)得模擬水樣中氨氮的含量為0.151mg/L（理論值為0.150mg/L）。運(yùn)用微型光譜儀在同樣的條件和環(huán)境下，繪制出氨氮含量在0-0.5mg/L濃度范圍內(nèi)所得標(biāo)準(zhǔn)曲線為y=0.8626x-0.087，R2=0.9969，檢測(cè)靈敏度為0.8626，回收率為99.36%，RSD為1.34%,最低檢出限為0.0045mg/L。測(cè)得水樣中氨氮含量為0.149mg/L（理論值為0.150mg/L）。關(guān)鍵詞:氨氮快速檢測(cè)，紫外可見分光光度法，微型光譜儀本科學(xué)生畢業(yè)設(shè)計(jì)（論文）ABSTRACTIIABSTRACTEnvironmentalprotectionisanimportantissueinmoderntimes.Waterqualitytestingisalsoanimportantissueintheenvironmentalprotection.Thetraditionaltechnologyneedsmuchwatersample,alotoftimeandcumbersomeoperationswhichdonotallowforonlinemonitoringofwaterquality.Micro-detectortechnologyisanemergingtechnology,withtheadvantagesofconvenient,fastoperationandlowwatersampleaddittion,whichcandetectthecontentofinvariouspollutantsinwater.Itisanimportantmeansinchemicalanalysistechnologyinthefuturewhichhasaverybroaddevelopingspace.Thispaperusingspectrophotometryfastdetectionwaterammonianitrogencontent,andrealizethereal-time,waterqualityon-linedetection.Inresearchsalicylicacidspectrophotometricmethod(GB/T8538-1995)basis,throughtheuseofmethylenechlorideacetonitrileuricacidsodiumhypochloriteinsteadofstandardbyusingcitricacid,sodiumasthreemaskingagent,regulatethesystemfromthreeaspectsofthePHoftheabovefactors,andstudytheammonianitrogencontentinthedetectionofinfluence,andoptimizecharacterizedgettheseinfluencefactors,exploretheoptimalvalueofasetofwithuv-visspectrophotometerfor10mindetectionmeans,getinthewaterintheammonianitrogencontentdetectionmethods.Optimizedforexperimentalconditions,usingthismethodwillammonianitrogeninthestandardsamplesindependentlydevelopedbasedonMS-IIminiaturespectrometeronwaterqualitymonitoringmulti-parametertesting,makestandardcurve.Waterqualitymonitoringmulti-parametertestingsimulationresultsandtheislandUV2450watercyanazineultravioletspectrophotometerdetectionresultsarecompared,theresultsshowedthatthewaterqualitymonitoringmulti-parametertestingresultsandislandUV2450jinisbasicallythesameerrorlessthan5%.Choosetwochlorineacetonitrileuricacidsodiumhypochlorite,insteadofmethylenechlorideacetonitrileuricacidsodiumsystemfor0.313mmol/Lconcentrationof0.2ml,additionamount.Reactiontemperaturemaintainat30,(0.5)withsodiumhydroxideregulationsystemforabout13PH.Thereactiontimefor10min.Inthebesttestconditions,usingislandUV2450uv-spectrophotometrycyanazineammonianitrogencontentthatinstrument,at0to0.5mg/Lconcentrationrangeofstandardcurveisy=0.4423x-0.0163R2=0.9984detectionsensitivityfor0.4423,recoveryis99.49%,RSD1.24%,lowestdetectlimitfor0.0087mg/L.Watermeasuredinthe本科學(xué)生畢業(yè)設(shè)計(jì)（論文）ABSTRACTIIIsimulationofammonianitrogencontentof0.151mg/L(theoretical0.150mg/L)for.Usingthesametermsinminiaturespectrometerandenvironmentandrenderingtheammonianitrogencontentin0-0.5mg/Lconcentrationrangeincomestandardcurveisy=0.8626x-0.087R2=0.9969detectionsensitivityfor0.8626,recove