GOODx27s緩沖液

1、Buffer Reference CenterBuffersUseful pHRangepKa(at 20)pKa(at 25)pKa(at 37)Reage ntGradeBPCGradeUltraGradeMES5.5-6.76.166.105.97M8250M2933M5287Bis-Tris5.8 彳2n/a6.506.36B9754B4429B7535ADA6.0-7.26.656.596.46A9883n/aA8074aces6.1 -7.56.886.786.54A9758A3594A7949PIPES6.1 -7.56.806.766.66P6757P1851P8203MOPS

2、O6.2-7.6n/a6.906.75M8389n/an/aBis-TrisPropane6.3-9.5n/a6.8, 9.0n/aB6755B4679B9410BES6.4-7.87.177.096.90B9879B4554B6420MOPS6.5-7.97.287.207.02M1254M3183M5162TES6.8-8.27.507.407.16T1375T5691T6541HEPES6.8-8.27.557.487.31H3375H4034H7273DIPSO7.0-8.2n/a7.607.35D9648n/aD0306MOBS6.9-8.3n/a7.60n/aM3295n/an/a

3、TAPSO7.0-8.2n/a7.607.39T9269T5566T0432Trizma7.0-9.08.208.067.72T1503T6066T6791HEPPSO7.1 -8.5n/a7.806.66H3137n/an/aPOPSO7.2-8.5n/a7.807.63P3405n/aP7088TEA7.3-8.3n/a7.80n/aT1377n/an/aEPPS7.3 -8.7n/a8.00n/aE9502E0276E1894Tric ine7.4-8.88.168.057.80T0377T5816T9784GIv-Glv7.5-8.9n/a8.20n/aG1002G3915G7278B

4、ici ne7.6 -9.08.358.268.04B3876n/aB8660HEPBS7.6-9.0n/a8.30n/aH6903n/an/aTAPS7.7-9.18.498.408.18T5130T5316T9659AMPD7.8-9.7n/a8.80n/aA9754n/aA9074TABS8.2-9.6n/a8.90n/aT1302n/an/aAMPSO8.3-9.7n/a9.009.10A6659n/aA7585CHES8.6-10.09.559.499.36C2885n/aC8210CAPSO8.9-10.3n/a9.609.43C2278n/aC8085AMP9 0-105n/a9

5、 70n/aA9879n/aA9199CAPS9 7-11 110 5610.4010 02C2632n/aC6070CABS10 0 11 4n/a10 70n/aC5580n/an/aoUseful pH Ra nges of Selected Biological Buffers (25 C, 0.1M)Trizma Buffer Table - pH vs. Temperature （三羥甲基氨基甲烷）pH at Temp eratureg/L for 0.05 M Solution5 C25 C37 CTrizma HCLTrizma Base7.767.206.917.020.67

6、7.897.307.026.850.807.977.407.126.610.978.077.507.226.351.188.187.607.306.061.398.267.707.405.721.668.377.807.525.321.978.487.907.624.882.308.588.007.714.442.658.688.107.804.022.978.788.207.913.543.348.888.308.013.073.708.988.408.102.644.039.098.508.222.214.369.188.608.311.834.659.288.708.421.504.90

7、9.368.808.511.235.139.478.908.620.965.329.569.008.700.765.47Phosphate Buffer Table -0.2M solution （磷酸鹽緩沖液）Po tassium Phos phate Mon obasic An hydrous g/LSodiumPhos phate DibasicHep tahydrate g/L23 CpHPo tassium Phos phate Mon obasic An hydrous g/LSodiumPhos phate DibasicHep tahydrate g/L23 CpH22.43.

8、495.710.8029.516.922.084.295.89.3632.737.021.605.375.97.9235.957.121.056.606.06.7238.637.220.408.056.15.5241.317.319.569.936.24.5643.467.418.6012.076.33.8445.077.517.6414.226.43.1246.687.616.4416.906.52.5248.557.715.0020.126.62.0449.097.813.5623.346.71.6849.897.912.2426.296.81.2750.818.0Citric Acid

9、-Sodium Citrate Buffer Solutio ns, pH 3.0.2Citric acid monohydrate, C6H8O7 ? H2O, M. wt. 210.14; 0.1M -solution contains 21.01 g/l. Na2HPO4, M. wt.141.98; 0.2M-solution contains 28.40 g/l, or Na2HPO4 ? 2H2O, M. wt. 178.05; 0.2M -solution contains 35.61 g/l.x ml 0.1M-citric acid and y ml 0.2M-Na2 HPO

10、4 mixed.pHx ml 0.1M-citric acidy ml 0.2-Na2 HPO42.689.1010.902.884.1515.853.079.4520.5513.275.3024.703.471.5028.503.667.8032.203.864.50135.50r4.061.4538.554.258.6041.404.455.9044.1014.653.2546.754.850.7049.305.048.5051.505.246.40153.60r5.444.2555.755.642.0058.005.839.5560.456.036.8563.156.233.9066.1

11、06.430.7569.256.627.2572.756.822.7577.257.017.6582.357.213.0586.957.49.1590.857.66.3593.65Citric acid mo nohydrate, C6H8O7 ? H2O, M. wt. 210.14; O.IM-solutio n contains 21.01 g/l. Trisodium citrate dihydrate, C6H5O7Na3 ? 2H2O, M. wt. 294.12; 0.1M - solution contains 29.41 g/l.x ml 0.1M-citric acid a

12、nd y ml O.IM-trisodium citrate mixed.pHx ml 0.1 M-citric acidy ml 0.1M-trisodium citrate3.082.018.03.277.522.53.473.027.03.668.531.53.863.536.54.059.041.04.254.046.04.449.550.54.644.555.54.840.060.05.035.065.05.230.569.55.425.574.55.621.079.05.816.084.06.011.588.56.28.092.0Sodium Acetate -Acetic Aci

13、d Buffer Solutions, pH 3.7 -5.6Sodium acetate trihydrate, CH3COONa ? 3H2O, M. wt. 136.09; 0.2Msolution con tai ns 27.22 g/l.x ml 0.2M-NaOAc and y ml 0.2M-HOAc mixed.pH, 18 Cx ml O.2M-NaOAcy ml 0.2M-HOAC3.710.090.03.812.088.04.018.082.04.226.573.54.437.063.04.649.051.04.859.041.05.070.030.05.279.021.

14、05.486.014.05.691.09.0Na2HPO4 -NaH2PO4 Buffer Solutions, pH 5.8 -8.0 at 25 CoNa2 HPO4 ? 2H2O, M. wt. 178.05; 0.2M-solutio n con tai ns 35.61 g/l. Na2 HPO4? 12H2O, M. wt. 358.22; 0.2M -solution contains 71.64 g/l. NaH2 PO4? H2O, M. wt.138.01; 0.2M-solutio n contains 27.6 g/l. NaH2 PO4? 2H2O, M. wt. 1

15、56.03; 0.2M- solution contains 31.21 g/l.x ml 0.2M-Na2 HPO4, y ml 0.2M-NaH2 PO4; diluted to 100 ml with H2O.pH,25 Cx ml 0.2M-Na 2HPO4y ml 0.2M-NaH 2PO45.84.046.06.06.1543.856.29.2540.756.413.2536.756.618.7531.256.824.525.57.030.519.57.236.014.07.440.59.57.643.56.57.845.754.258.047.352.65Imidazole (g

16、lyoxaline) -HCI buffer solutions, pH 6.2 .8 at 25 C1Imidazole, C3H4N2, M. wt. 68.08.25 ml 0.2M-imidazole (13.62 g/l), x ml 0.2M-HCI, diluted to 100 ml with H2O.pH,25 Cx ml 0.2M-HCl6.221.456.419.96.617.756.815.27.012.157.29.37.46.87.64.657.83.0Sodium Carbon ate -Sodium Bicarb on ate Buffer Soluti ons

17、, pH 9.210.81Na2CO3? 10H2O, M. wt. 286.2; 0.1M -solution contains 28.62 g/l. NaHCO3, M. wt. 84.0; 0.1M-solution contains8.40 g/l.x ml 0.1M-Na2CO3 and y ml 0.1M-Na2HCO3 mixed.Helpful FormulasP eree ntage by weight (w/v)(% buffer desired / 100) x final buffer volume (ml) = g of start ing material n eeded.Molar Solutio nsdesired mo