大氣PM與心血管疾病PPT學(xué)習(xí)教案

1、會(huì)計(jì)學(xué)1大氣大氣PM與心血管疾病與心血管疾病第1頁(yè)/共42頁(yè)Scanning electron microscopy (SEM) observations. The particulate matter (PM) samples collected in the rural site, in the urban site or the industrial site have been studied using SEM. According to their respective sampling sites and, therefore, origin, some morphologic

2、diff erences between the PM from the three collected samples were observed.第2頁(yè)/共42頁(yè)第3頁(yè)/共42頁(yè)最受關(guān)注的是可吸入顆粒物(PM 10) 粗顆粒物 PM2.5-10 細(xì)顆粒物（PM 2.5) 超細(xì)顆粒物 (PM0.1) PM簡(jiǎn)介簡(jiǎn)介第4頁(yè)/共42頁(yè)第5頁(yè)/共42頁(yè)第6頁(yè)/共42頁(yè)第7頁(yè)/共42頁(yè)第8頁(yè)/共42頁(yè)第9頁(yè)/共42頁(yè)第10頁(yè)/共42頁(yè)第11頁(yè)/共42頁(yè)第12頁(yè)/共42頁(yè)第13頁(yè)/共42頁(yè)第14頁(yè)/共42頁(yè)第15頁(yè)/共42頁(yè)第16頁(yè)/共42頁(yè)第17頁(yè)/共42頁(yè)第18頁(yè)/共42頁(yè)Fig. 1. Summa

3、ry of the pathways by which particulate matter promotes acute cardiovascular events and atherosclerosis. Mechanisms can be broadly divided into direct and indirect pathways.第19頁(yè)/共42頁(yè)第20頁(yè)/共42頁(yè)第21頁(yè)/共42頁(yè)第22頁(yè)/共42頁(yè)第23頁(yè)/共42頁(yè)第24頁(yè)/共42頁(yè)第25頁(yè)/共42頁(yè)大氣PM在體研究動(dòng)脈硬化？心功能？心律失常？凝血功能？機(jī)制研究（細(xì)胞培養(yǎng)、分子生物學(xué)、膜片鉗技術(shù)等）提出防治措施第26頁(yè)/共42

4、頁(yè)第27頁(yè)/共42頁(yè)注：與對(duì)照組相比a：P；與10g/ml組相比b： P；與100g/ml組比較c：P；與SOD+DMSO+PM100組比較d：P 前期實(shí)驗(yàn)結(jié)果（一）內(nèi)皮完整各組間收縮率比較第28頁(yè)/共42頁(yè) 注：各組間比較 前期實(shí)驗(yàn)結(jié)果（二）去內(nèi)皮各組間收縮率比較第29頁(yè)/共42頁(yè)注：與對(duì)照組相比a：P、A ：P；與10g/ml組相比b： P、B ：P；與100g/ml組相比c：P 前期實(shí)驗(yàn)結(jié)果（三） 內(nèi)皮完整各組間舒張率比較第30頁(yè)/共42頁(yè)第31頁(yè)/共42頁(yè)入細(xì)胞核Nrf2入細(xì)胞核ARE介導(dǎo)的二相酶基因如HO-1等表達(dá)蛋白、脂質(zhì)、核酸過(guò)氧化、線粒體受損大氣PM2.5致內(nèi)皮細(xì)胞氧化應(yīng)激可能

5、機(jī)制（參考相關(guān)文獻(xiàn)完成）PMROSMAPK PI3K/AktPKCKeap1/Nrf2科學(xué)設(shè)想第32頁(yè)/共42頁(yè)Nrf2Keap-1胞胞 漿漿細(xì)胞核核膜細(xì)胞核核膜ARE二相酶基因表達(dá)增多二相酶二相酶第33頁(yè)/共42頁(yè)實(shí)驗(yàn)結(jié)果（一）第34頁(yè)/共42頁(yè)實(shí)驗(yàn)結(jié)果（二）第35頁(yè)/共42頁(yè)Western blot analysis shows that protein expression of HO-1and Nrf2 was induced with PM2.5 treatment in HUVEC cells. B. densitometric analyses of HO-1 and Nrf2、

6、Keap1 protein amount of Nrf2, Keap1, or HO-1 protein was normalized to GAPDH protein， Results are expressed as -fold induction compared with that of control. Data expressed are . (n=3). *, P0.05; *,P 0.01 versus control HUVEC cells，#, PPM2.5 concentration(g/ml)contral50100 protein expression of HO-1/GAPDH(%)0.0.51.01.52.02.5 *#PM2.5 concentration(g/ml)contral50100protein expression of Keap1/GADPH(%)0.0.2.4.6.81.01.2PM2.5 concentration(g/ml)contral50100protein ex