熒光素激發(fā)發(fā)射波長(zhǎng)

1、熒光素名稱激發(fā)波長(zhǎng)(nm)發(fā)射波長(zhǎng)(nm)備注1,5 IAEDANS3364901,8-ANS3724804-Methylumbelliferone3855025-carboxy-2,7-dichlorofluorescein5045295-Carboxyfluorescein (5-FAM)4925185-Carboxynapthofluorescein (pH 10)512/598563/668Ratio Dye, pH5-Carboxytetramethylrhodamine (5-TAMRA)5425685-FAM (5-Carboxyfluorescein)4925185-HAT (

2、HydroxyTryptamine)370-415520-5405-Hydroxy Tryptamine (HAT)370-415520-5405-ROX (carboxy-X-rhodamine)5785676045915-TAMRA (5-Carboxytetramethylrhodamine)5485425525686-Carboxyrhodamine 6G5185436-CR 6G5185436-JOE5205487-Amino-4-methylcoumarin3514307-Aminoactinomycin D (7-AAD)5466477-Hydroxy-4-methylcouma

3、rin360449, 4559-Amino-6-chloro-2-methoxyacridine412, 430471,474ABQ344445Acid Fuchsin540630ACMA (9-Amino-6-chloro-2-methoxyacridine)412, 430471,474Acridine Orange + DNA502526Acridine Orange + RNA460650Acridine Orange, both DNA & RNA440-480520-650Acridine Red455-600560-680Acridine Yellow470550Acri

4、flavin436520AcriflavinFeulgen SITSA355-425460Aequorin (Photoprotein)466PhotoproteinAFPs - AutoFluorescent Protein - (Quantum Biotechnologies) see sgGFP, sgBFPAlexa Fluor 350?346342:Alexa Fluor 430?431540Alexa Fluor 488?495, 492519, 520Alexa Fluor 532?531,532553, 554Alexa Fluor 546?556, 557572, 573Al

5、exa Fluor 568?577, 578603Alexa Fluor 594?590, 594617, 618Alexa Fluor 633?632650Alexa Fluor 647?647666Alexa Fluor 660?668698Alexa Fluor 680?679702Alizarin Complexon530-560,580580,624-645Alizarin Red530-560580Allophycocyanin (APC)630, 645655, 660AMC, AMCA-S345445AMCA (Aminomethylcoumarin)345347425444A

6、MCA-X353442Aminoactinomycin D555655Aminocoumarin346350442445Aminomethylcoumarin (AMCA)345347425444Anilin Blue600Anthrocyl stearate360-381446APC (Allophycocyanin)630, 645655, 660APC-Cy7625-650755APTRA-BTC = Ratio Dye, Zn2+466/380520/530Ratio Dye, Zn2+APTS424505Astrazon Brilliant Red 4G500585Astrazon

7、Orange R470540Astrazon Red 6B520595Astrazon Yellow 7 GLL450480Atabrine436490ATTO- TAG? CBQCA465560ATTO- TAG? FQ486591Auramine460550Aurophosphine G450580Aurophosphine450-490515BAO 9 (Bisaminophenyloxadiazole)365395BCECF (high pH)492, 503520,528BCECF (low pH)482520BerberineSulphate430550Beta Lactamase

8、409447, 520BFP blue shifted GFP (Y66H)Blue Fluorescent Protein381,382,383445, 447,448blue shifted GFP(Y66H)Blue FluorescentProteinBFP/GFP FRETBimane398490Bisbenzamide360461Bisbenzimide (Hoechst)360461bis-BTC = Ratio Dye, Zn2+455/405529/505Ratio Dye, Zn2+Blancophor FFG390470Blancophor SV370435BOBO? -

9、1462481BOBO? -3570602Bodipy 492/515490515Bodipy 493/503533549Bodipy 500/510509515Bodipy 505/515502510Bodipy 530/550528547Bodipy 542/563543563Bodipy 558/568558569Bodipy 564/570564570Bodipy 576/589579590Bodipy 581/591584592Bodipy 630/650-X625642Bodipy 650/665-X647665Bodipy 665/676605676BodipyFl504, 50

10、5511,513Bodipy FL ATP505514BodipyFl-Ceramide505511Bodipy R6G SE528547Bodipy TMR542574Bodipy TMR-X conjugate544573Bodipy TMR-X, SE544570Bodipy TR589617Bodipy TR ATP591620Bodipy TR-X SE5886161BO- PRO? -1462481BO- PRO? -3544570Brilliant Sulphoflavin FF430520BTC - Ratio Dye Ca2+464/401533/529Ratio Dye C

11、a2+BTC-5N - atio Dye, Zn2+459/417517/532Ratio Dye, Zn2+Calcein494517Calcein Blue373440Calcium Crimson?588, 589611,615Calcium Green501,506531Calcium Green-1 Ca2+ Dye506531Ca2+ DyeCalcium Green-2 Ca2+506/503536Ca2+Calcium Green-5N Ca2+506532Ca2+Calcium Green-C18 Ca2+509530Ca2+Calcium Orange549575576Ca

12、lcofluor White385, 395,405437, 440,445Carboxy-X-rhodamine (5-ROX)576601Cascade Blue?377398399420423Cascade Yellow399400550552Catecholamine410470CCF2 (GeneBlazer)CFDA494520CFP - Cyan Fluorescent Protein430, 433,436, (453)474, 475,476, (501)Cyan FluorescentProteinCFP/YFP FRETChlorophyll480650Chromomyc

13、in A436-460470Chromomycin A445575CL-NERF (Ratio Dye, pH)504/514540Ratio Dye, pHCMFDA494520Coelenterazine Ca2+ Dye, bioluminescence(429)465Ca2+ Dye, bioluminescence, native moleculeCoelenterazinecp (Ca2+ Dye,)(430)442Ca2+ Dye, bioluminescenceCoelenterazine f(437)473Ca2+ Dye, bioluminescenceCoelentera

14、zinefcp452Ca2+ Dye, bioluminescenceCoelenterazine h(437)464Ca2+ Dye,bioluminescenceCoelenterazinehcp(433)444Ca2+ Dye, bioluminescenceCoelenterazineip441Ca2+ Dye, bioluminescenceCoelenterazine n(431)467Ca2+ Dye, bioluminescenceCoelenterazine O460575CoumarinPhalloidin387470C-phycocyanineCPM Methylcoum

15、arin384469MethylcoumarinCTC400-450602CTC FormazanCy2?489506Cy3.1 8554568Cy3.5?581598Cy3?514552554566570Cy5.1 8649666Cy5.5?675695Cy5?649670Cy7?710, 743767, 805Cyan GFP433 (453)475 (501)cyclic AMP Fluorosensor (FiCRhR)500517CyQuant Cell Proliferation Assay480520Cell ProliferationAssayDabcyl453Dansyl34

16、0578Dansyl Amine337517DansylCadaverine335518Dansyl Chloride372518Dansyl DHPE336517Dansyl fluoride356noneDAPI359461Dapoxyl403580Dapoxyl 2374574Dapoxyl 3373574DCFDA504529DCFH (DichlorodihydrofluoresceinDiacetate)505535DDAO463607DHR (Dihydorhodamine 123)505534Di-4-ANEPPS496705Di-8-ANEPPS (non-ratio)488

17、498605713DiA (4-Di-16-ASP)456591DichlorodihydrofluoresceinDiacetate (DCFH)505535DiD - Lipophilic Tracer644665Lipophilic TracerDiD (DiIC18(5)644665DIDS341415Dihydorhodamine 123 (DHR)505535DiI (DiIC18(3)549, 551565Dinitrophenol349DiO (DiOC18(3)484, 487501,502DiR748780Lipophilic TracerDiR (DiIC18 (7)75

18、0779DM-NERF (high pH)497/510540Ratio Dye, pHDNP349Dopamine340490-520DsRed558583Red fluorescent proteinDTAF494520DY-630-NHS621660Hemicyanelabel for proteins andDNADY-635-NHS634664Hemicyanelabel for proteins andDNAEBFP383447Enhanced BlueFluorescent ProteinECFP436474Enhanced CyanFluorescent ProteinEGFP

19、488, 498507, 516Enhanced GreenFluorescent ProteinELF 97345530Eosin524545Erythrosin529, 532554, 555Erythrosin ITC529555Ethidium Bromide510, 523595, 605Ethidiumhomodimer -1 (EthD-1)528617Euchrysin430540EukoLightEuropium (III) chlorideEYFP513, 520527, 532Enhanced YellowFluorescent ProteinFast Blue36044

20、0FDA494520Feulgen (Pararosaniline)570625FIF (Formaldehyd Induced Fluorescence)405433FITC490, 494520, 525FITC Antibody493517Flazo Orange375-530612Fluo-3480506,506520, 527Fluo-4494516Fluorescein (FITC)490, 494520, 525Fluorescein Diacetate494520Fluoro-Emerald495524Fluoro-Gold (Hydroxystilbamidine)36153

21、6Fluor-Ruby555582FluorX494520FM 1- 43?479598FM 4-46515640Fura Red? (high pH)572657Fura Red?/Fluo -3Fura-2, high calcium335505Excitation ratio dyeFura-2, low calcium363512Excitation ratio dyeFura-2/BCECFGenacryl Brilliant Red B520590Genacryl Brilliant Yellow 10GF430485Genacryl Pink 3G470583Genacryl Y

22、ellow 5GF430475GeneBlazer (CCF2)GFP (S65T)498516GFP red shifted (rsGFP)498516GFP wild type, non-UV excitation (wtGFP)475509GFP wild type, UV excitation (wtGFP)395509GFPuv385508Gloxalic Acid405460Granular Blue355425Haematoporphyrin530-560580Hoechst 33258345487Hoechst 33342347483Hoechst 34580392440HPT

23、S355465Hydroxycoumarin325-360386-455Hydroxystilbamidine (FluoroGold)361536Hydroxytryptamine400530ndo-1, high calcium330401Emission ratio dyendo-1, low calcium346475Emission ratio dyendodicarbocyanine (DiD)644665ndotricarbocyanine (DiR)748780ntrawhiteCf360430JC-1514529JO-JO-1530545JO-PRO-1532544Laser

24、Pro795812Laurodan355460LDS 751 (DNA)543712LDS 751 (RNA)590607 rLeucophor PAF370430Leucophor SF380465Leucophor WS395465LissamineRhodamine572, 577591,592LissamineRhodamine B577592LIVE/DEAD Kit Animal CellsCalcein/Ethidiumhomodimer494528517617for more details refer to LOLO-1566580LO-PRO-1568581Lucifer

25、Yellow425, 428528, 536,540Lyso Tracker Blue373422Lyso Tracker Blue-White466536Lyso Tracker Green504, 534511,551Lyso Tracker Red490516Lyso Tracker Yellow551576LysoSensor Blue374424LysoSensor Green442505LysoSensor Yellow/Blue384540Mag Green507531Magdala Red (Phloxin B)524600Mag-Fura Red483/427659/631R

26、atio Dye, Mg2+Mag-Fura-2369/329369/330508511/491Ratio Dye Ca2+Ratio Dye Mg2+Mag-Fura-5369/330369/332505/500505/482Ratio Dye, Ca2+Ratio Dye, Mg2+Mag-Indo-1349/328349/330480/390480/417Ratio Dye, Ca2+Ratio Dye, Mg2+Magnesium Green506, 507531Magnesium Orange550575Malachite Green628Marina Blue362459Maxil

27、on Brilliant Flavin 10 GFF450495Maxilon Brilliant Flavin 8 GFF460495Merocyanin555578Methoxycoumarin360410Mitotracker Green FM490516Mitotracker Orange551576Mitotracker Red578599Mitramycin450470Monobromobimane398490Monobromobimane (mBBr-GSH)398500Monochlorobimane380461MPS (Methyl Green PyronineStilben

28、e)364395NBD466539NBD Amine450530Nile Red515-555,559590, 640Nitrobenzoxadidole465510-650Noradrenaline340490-520Nuclear Fast Red289-530580Nuclear Yellow365495Nylosan Brilliant Iavin E8G460510Oregon Green503522Oregon Green 488-X494517Oregon Green?503522Oregon Green? 488490, 493514, 520Oregon Green? 500

29、497517Oregon Green? 514506526Pacific Blue405455Pararosaniline (Feulgen)570625PBFI340/380420Excitation ratio dyePE-Cy5488670PE-Cy7488755, 767PerCP488675PerCP-Cy5.5488710PE-TexasRed Red 613488613Phloxin B (Magdala Red)524600Phorwite AR360430Phorwite BKL370430Phorwite Rev380430Phorwite RPA375430Phosphi

30、ne 3R465565PhotoResist365610Phycoerythrin B PE546-565575Phycoerythrin R PE565578PKH26 (Sigma)551567PKH67496520ChromaPMIA341376Pontochrome Blue Black535-553605POPO-1433457POPO-3533574PO-PRO-1435455PO-PRO-3539567Primuline410550Procion Yellow470600PropidiumIodid (PI)(305),536, 538617PyMPO412, 415561,56

31、4,570Pyrene360387Pyronine410540Pyronine B540-590560-650Pyrozal Brilliant Flavin 7GF365495QSY 7560Quinacrine Mustard440510Red 613 PE-TexasRed488613Resorufin571584, 585RH 414532716Rhod-2552576Rhodamine550573Rhodamine 110496, 497520Rhodamine 123507529Rhodamine 5 GLD470565Rhodamine 6G525555Rhodamine B54

32、0625Rhodamine B 200523-557595Rhodamine B extra550605Rhodamine BB540580Rhodamine BG540572Rhodamine Green502527RhodaminePhallicidine558, 542575, 565RhodaminePhalloidine542565Rhodamine Red570590Rhodamine WT530555Rose Bengal525, 540550-600R-phycocyanineR-phycoerythrin (PE)565578rsGFP473509red shifted GF

33、P(S65T)S65A471504S65C479507S65L484510S65T488511Sapphire GFP395511SBFI340/380420Excitation ratio dyeSerotonin365520-540Sevron Brilliant Red 2B520595Sevron Brilliant Red 4G500583Sevron Brilliant Red B530590Sevron Orange440530Sevron Yellow L430490sgBFP?387450sgBFP? (super glow BFP)387450Quantum ' s

34、SuperGlo?GFPAFPssgGFP?474488sgGFP? (super glow GFP)474509Quantum ' sSuperGlo?GFPAFPsSITS336436Ion ChannelsSITS (Primuline)395-425450SITS (StilbeneIsothiosulphonic Acid)365460SNAFL calcein506/535535/620Ratio Dye, pHSNAFL-1508/540543/623Ratio Dye, pHSNAFL-2514/543546/630Ratio Dye, pHSNARF calcein5

35、52/574590/629Ratio Dye, pHSNARF1576/548635/587Excitation and emission ratio dyeSodium Green506, 507532Na+, K+SpectrumAqua433,/53480/55VysisSpectrumGreen497/30,509/31538/44,524/56VysisSpectrumOrange559/38,560588/48VysisSpectrum Red587,587/35612,612/51SPQ (6-methoxy-N-(3-sulfopropyl)quinolinium)344443

36、Stilbene335440Sulphorhodamine B can C520595Sulphorhodamine G Extra470570SYTO 11508, 510527, 530Dye for DNA, RNASYTO 12499, 500522, 519Dye for DNA, RNASYTO 13488, 491509, 514Dye for DNA, RNASYTO 14517, 521549, 547Dye for DNA, RNASYTO 15516, 518546, 555Dye for DNA, RNASYTO 16488, 494518, 525Dye for DN

37、A, RNASYTO 17621634Dye for DNASYTO 18490, 493507, 527Dye for DNA, RNASYTO 20512530Dye for DNASYTO 21494517Dye for DNASYTO 22515535Dye for DNASYTO 23499520Dye for DNASYTO 24490515Dye for DNASYTO 25521556Dye for DNASYTO 40420441Dye for DNASYTO 41430454Dye for DNASYTO 42433460Dye for DNASYTO 43436467Dye for DNASYTO 44446471Dye for DNASYTO 45452484Dye for DNASYTO 59622645Dye