SOD及NF-κB在梗阻性黃疸大鼠肝臟損傷中的作用

1、SOD及NF-KB在梗阻性黃疸大鼠肝臟損傷中的作用【摘要】 目的：檢測(cè)超氧化物歧化酶(superoxide dismutase , sod)活力及核因子-Kb(nuclear factor-Kb, nf-Kb)在梗阻性 黃疸大鼠肝臟中的表達(dá)，探討其在梗阻性黃疸大鼠肝臟損害中的作 用。方法：采用膽總管結(jié)扎法構(gòu)建梗阻性黃疸大鼠模型，檢測(cè)血清 總膽紅素(totalbilirubin ，tb)、直接膽紅素(directbilirubin ，db)禾口谷丙轉(zhuǎn)氨酶(alanine aminotransferase， alt)，以及肝臟組織行 he 病理切片檢查鑒定梗阻性黃疸大鼠模型。肝臟組織勻漿 后檢測(cè)組

2、織中總 sod 和 cuzn-sod 活力，以免疫組織化學(xué)方法檢測(cè) nf-Kb 在肝臟中的表達(dá)。結(jié)果：膽道結(jié)扎(bile duct ligation , bdl) 組血清 tb、db 及alt 明顯增高(p0.01);膽道結(jié)扎組肝組織中總 sod 和 cuzn-sod 活力較假手術(shù)組明顯減低(p0.01)。膽道結(jié)扎組 nf-Kb p65 蛋白激活并出現(xiàn)核移位，19 d 組較 7 d 組更加顯著。 結(jié)論：脂質(zhì)過(guò)氧化是梗阻性黃疸器官損傷的最重要的機(jī)制之一，而 sod 活力的降低及 nf-Kb 的激活，在梗阻性黃疸肝臟過(guò)氧化損害中 具有重要意義?！娟P(guān)鍵詞】超氧化物歧化酶；核因子-Kb；梗阻性黃疸；

3、肝臟roles of sod and nf-Kb in liver injury of obstructivejaundice in rats/xu hai-bo , gong peng./medicalinnovationof china , 2012, 9(12) : 003-005abstract objective : to determine the activities of sodand the expression of nuclear factor-Kb(nf-Kb) in rats with obstructivejaundice induced by bile duct

4、ligation(bdl) , and elucidate themechanisms of hepatic injury due to obstructive jaundice. methods：the animal model of obstructive jaundicein rats were made to be bile duct ligation. all rats were sacrificed on the7th day and 19th day after bdl. the blood and liver tissue samples wereobtained. total

5、 bilirubin(tb),direct bilirub in( db), ala nine aminotran sferase(alt) inserum were detected and liver tissue he pathology section wereevaluated. sod en zyme activity was detected by sod kit. the detecti onof activati on of nf-kb was performed by an immunohistochemicalmethod. results : in bdl animal

6、s serum, tb , db , and alt sig ni fica ntlyin creased compared with the sham group (p0.01). by he pathologysection , we observed that in the bdl group , in liver sig ni fica nt damagesig ns occurred , and the cha nge on 19th day after bdl was moreobvious. the activities of normal sod and cuzn-sod decreased comparedto the shamgroup(p0.01) , and the decrease on 19th day after bdl wasmore significant.in bdl group , nf-Kbp65activation and nuclear displacement response , on 19th day after b