高壓氧預(yù)適應(yīng)對老齡大鼠急性全腦缺血的影響

1、高壓氧預(yù)適應(yīng)對老齡大鼠急性全腦缺血的影響摘要目的 觀察高壓氧預(yù)適應(yīng)（hyperbaric oxygen preconditioning）對老齡大鼠急性全腦缺血后認(rèn)知功能的影響，磁共振成像分析大腦皮質(zhì)影像學(xué)改變。方法 24只老齡雄性SD大鼠隨機分為4組（n=6），正常對照組（C組）、單純高壓氧組（H組）、單純?nèi)毖M（E1組）、高壓氧預(yù)適應(yīng)缺血組（E2組），E1組、E2組采用改良Pulsinelli四血管閉塞法建立急性全腦缺血模型， E2組建模前和H組給予連續(xù)5天，每天1小時2個標(biāo)準(zhǔn)大氣壓（2.0ATA）高壓氧處理，各組大鼠分別行橫斷位及冠狀位T1WI、T2WI掃描；Morris水迷宮測試學(xué)習(xí)記憶

2、功能：逃避潛伏期和目標(biāo)象限游泳時間百分比。結(jié)果 C組、H組大腦未見明顯缺血梗死灶，E1組雙側(cè)皮質(zhì)區(qū)可見明顯弧形缺血梗死灶，E2組雙側(cè)皮質(zhì)區(qū)也可見弧形缺血梗死區(qū)，面積較E1組小。E1組逃避潛伏期長于E2組（P0.05），E2組逃避潛伏期長于C組、H組（P0.05），C組和H組之間差別無統(tǒng)計學(xué)意義。E1組目標(biāo)象限游泳時間百分比較E2組短（P0.05），E2組短于C組、H組，C組和H組之間差別無統(tǒng)計學(xué)意義。結(jié)論 給予連續(xù)高壓氧預(yù)適應(yīng)，能減少老齡大鼠急性全腦缺血后超急性期大腦皮質(zhì)缺血梗死面積，提高全腦缺血后的認(rèn)知功能水平。關(guān)鍵詞：高壓氧；預(yù)適應(yīng)；認(rèn)知功能；老齡大鼠；磁共振成像Effects Of Hy

3、perbaric Oxygen Preconditioning On Aged Rats After Acute Cerebral IschemiaAbstractObjective To investigate the effects of hyperbaric oxygen preconditioning on the cognitive function of aged rats after acute cerebral ischemia，and analyze the changes in the cerebral cortex by magnetic resonance imagin

4、g（MRI）Methods 32 aged male SD rats were randomly divided into group C (normal control group), H group (pure hyperbaric oxygen group), E1 group (simply ischemic group), E2 group (hyperbaric oxygen preconditioning ischemia group)，eight rats in each group. E1 and E2 groups were established acute cerebr

5、al ischemia model by modified Pulsinelli four-vessel occlusion method. The E2 group before setting up acute cerebral ischemia model and the H group were given hyperbaric oxygen treatment once a day for 5 days. Each group were scan axial line and coronal in T1WI and T2WI. The learning and memory were

6、 evaluated with Morris water maze: escape latency and percentage of swimming distance in the platform quadrant.Results There were no obvious ischemic brain infarction in C and H group，there was clearly arc-shaped bilateral cortex ischemic infarct area in E1 group，the ischemic infarct area in E2 was

7、smaller than which the E1 group；Escape latency of E1 group was longer than E2 group（P0.05），and E2 group was longer than C or H group（P0.05）.Swimming distance in the platform quadrant of E1 group was shorter than that of E2 group（P0.05），and E2 group was shorter than C or H group. There were no significant Statistics differences between C and H group.Conclusion Given continuous hyperbaric oxygen preconditioning can reduce the ischemic infarct area in aged rats after acute global cerebral ischemia，improve cognitive fun