樹枝狀大分子在生物成像中的應(yīng)用

1、#Group Number: 33Reporter: 劉濤劉濤 BA10020009Member: 曹志華曹志華 SA10005908Bioimaging applications of Dendrimers#仿生仿生Mimicking nature#Typical linear polymerTypical dendrimer#Synthetic rout: divergent #Synthetic rout: convergent #-COOH-OH-NH2-N3-alkynyl-SH-ethylenePAMAM#GeneDrugVaccineTissue EngineeringBioim

2、aging#FITC RhredRhodamine #Achievements in the field of MRIThe Nobel Prize Winners in Physiology or Medicine in 2003 for their Discoveries concerning MRI#R1 = (DOTA)Gd R2 = Folic AcidHyperbranched Polyester Boltorn H40Preparation of H40-Based Unimolecular Micelles for Targeted Drug Delivery and MRIO

3、HH406060CLROPEt3N/THFOEGMAAzPMAAlkynyl-(DOTA)GdAlkynyl-folateBrBrOOH40OOOmOOOON3Oabn60OH40OOOmOOOONOabnONONNNNR1R2c#0.000.020.040.060.080.100.00.51.01.52.02.5y = 19.12 x + 0.1242y = 17.81 x + 0.0732y = 2.01 x + 0.15261/T1 (s-1)Gd (mM) Alkynyl-(DOTA)Gd Micelles-Gd Micelles-Gd&FA1/T1 (s-1)b)L1: Al

4、kynyl-(DOTA)GdL2: H40-based Unimolecular Micelles with (DOTA)GdL3: H40-based Unimolecular Micelles with (DOTA)Gd and FAIn Vitro Enhancement Effect of Unimolecular Micelles on MRIa)#01020304050602736455463 Signal Noise RatioTime / min Alkynyl-(DOTA)Gd Micelles-Gd Micelles-Gd&FALiver slices from w

5、hite rabbitIn Vivo Enhancement Effect of Unimolecular Micelles on MRIa)b)c)d)e)f)g)h)pre0.5 h1 h2 h4 h10 h20 h30 h#References:1. Nature Biotechnology 23, 1517 - 1526 (2005) 2. Journal of the American Chemical Society, 132, 18158-18167 (2010)3. Journal of Physical Chemistry B, 114 1751-1756 (2010)4. Journal of the American Chemical Society, 133, 1250712517 (2011)5. Journal of the American Chemical Society, 133, 2050720520 (2011)6. Pharmaceutical Research, 28,