補(bǔ)骨脂乙素作用機(jī)制 - Medchemexpress - MCE中國

1、Product Data SheetIsobavachalconeCat. No.: HY-13065CAS No.: 20784-50-3分式: CHO分量: 324.37作靶點(diǎn): Akt; Reactive Oxygen Species; Apoptosis; Autophagy作通路: PI3K/Akt/mTOR; Immunology/Inflammation; Metabolic Enzyme/Protease; NF-B;Apoptosis; Autophagy儲存式: 4C, protect from light* In solvent : -80C, 6 months; -20

2、C, 1 month (protect from light)溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 11 mg/mL (33.91 mM; Need ultrasonic and warming)SolventMass1 mg 5 mg 10 mgConcentration制備儲備液1 mM 3.0829 mL 15.4145 mL 30.8290 mL5 mM 0.6166 mL 3.0829 mL 6.1658 mL10 mM 0.3083 mL 1.5414 mL 3.0829 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲

3、備液的保存式和期限：-80C, 6 months; -20C, 1 month (protect from light)。-80C 儲存時(shí)，請?jiān)?6 個(gè)內(nèi)使，-20C 儲存時(shí)，請?jiān)?1 個(gè)內(nèi)使。BIOLOGICAL ACTIVITY物活性 Isobavachalcone (Corylifolinin) 來于補(bǔ)脂， 種 Akt 信號通路的有效抑制劑，可誘導(dǎo)類癌細(xì)胞凋亡 (以 IC50 值為 7.92 M 抑制 OVCAR-8 癌細(xì)胞長)，具有抗癌和抗增殖活性。Isobavachalcone 還能誘導(dǎo) OVCAR-8 細(xì)胞中活性氧的產(chǎn)。IC & Target IC50: 7.92 M (OVCAR

4、-8 cell)1體外研究Isobavachalcone (6.0-48.0 M; 72 hours; OVCAR-8, PC3, A549, MCF-7, L-02 and HUVEC cells) treatment inhibits theproliferation of human cancer cells. Isobavachalcone inhibits PC3, A549, MCF-7, L-02 and HUVEC cells growth withIC50 values of 15.06 M, 32.2 M, 28.29 M, 31.61 M and 31.3 M, resp

5、ectively1.Isobavachalcone (0-18 M; 6 hours; OVCAR-8 and PC3 cells) treatment results in a concentration-and time-dependent down-regulation of the Ser-473 phosphorylation of Akt and GSK3b phosphorylation1.Isobavachalcone (0-18 M; 72 hours; OVCAR-8 and PC3 cells) treatment causes apoptosis via caspase

6、- and ROS-involved mitochondrial pathway1.Page 1 of 2 www.MedChemECell Proliferation Assay1Cell Line: OVCAR-8, PC3, A549, MCF-7, L-02 and HUVEC cellsConcentration: 6.0-48.0 MIncubation Time: 72 hoursResult: Inhibited the proliferation of human cancer cells.Western Blot Analysis1Cell Line: OVCAR-8 or

7、 PC3 cellsConcentration: 0 M, 6 M, 12 M, and 18 MIncubation Time: 6 hoursResult: A concentration-and time-dependent down-regulation of the Ser-473phosphorylation of Akt. GSK3b phosphorylation was also inhibited in a concentration-and time-dependent manner.Apoptosis Analysis1Cell Line: OVCAR-8 cells

8、and PC3 cellsConcentration: 0 M, 6 M, 12 M, and 18 MIncubation Time: 72 hoursResult: Led to dose dependent increase of apoptosis.體內(nèi)研究 Isobavachalcone (20 mg/kg; intraperitoneal injection; for 0.5-24 hours; female Kunming mice) treatment results in anincrease in blood glucose levels, reaching a maxim

9、um within 1 hour and maintaining until 4 hours post-dosing1.Animal Model: Seven-week-old specific pathogen-free female Kunming mice (18-22 g)1Dosage: 20 mg/kgAdministration: Intraperitoneal injection; for 0.5, 1, 2, 4, 6, 8, 12, 24 hoursResult: Increased in blood glucose levels.戶使本產(chǎn)品發(fā)表的科研獻(xiàn) Int Immun

10、opharmacol. 2013 Jan;15(1):38-41.See more customer validations on HYPERLINK www.MedChemE www.MedChemEREFERENCES1. Jing H, et al. Abrogation of Akt signaling by Isobavachalcone contributes to its anti-proliferative effects towards human cancer cells. Cancer Lett. 2010Page 2 of 3 www.MedChemEAug 28;294(2):167-77.McePdfHeightCaution: Product has not been fully validated for medical a