生物反應(yīng)器Newtechnologiesincellculture實用教案

1、Content Introduction Comparison Sensor technologies第1頁/共34頁第一頁，共33頁。Introduction - bioreactors第2頁/共34頁第二頁，共33頁。Introduction - bioreactors Capable of being operated aseptically for a number of days, weeks and even months Capable of generating repeatable process conditions for a number of days, weeks

2、and even months Adequate aeration and agitation should be provided to meet the metabolic requirements of the micro-organism or cell pH, Oxygen, Temperature and other control should be provided Sampling facilities should be provided Should be suitable for a range of processes Should be constructed to

3、 ensure smooth internal surfaces (cleanability)第3頁/共34頁第三頁，共33頁。-Flask第4頁/共34頁第四頁，共33頁。 Test Tubes, Shake flasks. Increased aeration. Reduced evaporative loss. Solid support , Re-useable / Autoclavable. May be used with the SensorDish Reader.-Flasks are Shake Flasks第5頁/共34頁第五頁，共33頁。 -Flask Configura

4、tionsFrom 100ul to 4.0ml第6頁/共34頁第六頁，共33頁。SensorDish Reader , SDR第7頁/共34頁第七頁，共33頁。SensorDish Reader , SDR1.Non-invasive, online.2.pH or Dissolved Oxygen.3.No Calibration Required.6-well24-well第8頁/共34頁第八頁，共33頁。ExpandableAdditional SDRs link into the Basic Set.Up to 10 SDRs on one Laptop.No additional

5、software required for expansion.第9頁/共34頁第九頁，共33頁。SDRs Integrate with -Flasks-Flasks Integrate with the SensorDishReader1.Eliminate Well-plate inconsistency.2.Reduce and equalize evaporative losses across well-plates.3.Obtain online pH or dissolved oxygen data for all wells.第10頁/共34頁第十頁，共33頁。DO profi

6、les (all 24 for each well-plate)Polystyreneu-Flask第11頁/共34頁第十一頁，共33頁。SensorFlaskReader , SFR第12頁/共34頁第十二頁，共33頁。The SensorFlask Reader, SFR Shaker-mounting blue-tooth sensor platform. Shake Flasks with DO and pH sensors.(also single DO or pH if desired) Multiple volumes (125/250/500/1000 ml). Up to 9

7、 flasks on the platform at one time.第13頁/共34頁第十三頁，共33頁。Microbial and Mammalian Applications Microbial Applications:Monitor pH and DO levels. Ensure aerobic growth.Follow carbon source utilization and exhaustion.Adjust orbiter speeds to maintain DO levels for better scalability. Mammalian Application

8、s: Accurately monitor pH /pCO2 levels. Adjust bicarbonate levels more accurately.Monitor DO levels, adjust orbiter speeds for better scalability.第14頁/共34頁第十四頁，共33頁。Software第15頁/共34頁第十五頁，共33頁。Individual Flask Calibration第16頁/共34頁第十六頁，共33頁。Complete Hardware Hardware Includes:Platform.Batteries.Bluetoo

9、th adapter.Software.Clamps. Bluetooth range of 15ft. Battery life 3 months on a single charge. (platform can operate on single battery)第17頁/共34頁第十七頁，共33頁。The RAMBIO1000 (Resonant Acoustic Mixing)第18頁/共34頁第十八頁，共33頁。Resonant Acoustic Mixing All energy goes into mixing the contents of the flask 6 fold

10、better OTRs than orbital shakers due to increased headspace exchange Up to 50% fill volume in standard flasks第19頁/共34頁第十九頁，共33頁。The Applikon RAMAccessories: Clamps and Oxy-pump Stoppers250ml1000ml第20頁/共34頁第二十頁，共33頁。Single use applications Small scale options, more R&DAppliFlex CellReady第21頁/共34頁

11、第二十一頁，共33頁。Sensor technologies Traditional sensor pH and DO Sterilized via autoclave第22頁/共34頁第二十二頁，共33頁。Sensor technologies - Fluorescent Fluorescent light is measure for pH and DO第23頁/共34頁第二十三頁，共33頁。Fluorescence pro/con Pre-sterilized Pre-calibrated (pH) Single use + reusable (DO) Measurement throu

12、gh wall of vessel pH range limited Not backwards compatible (controller) Can be influenced by medium (pH) Additional costPROCON第24頁/共34頁第二十四頁，共33頁。New technologies AppliFlex reusableAppliFlex single sensorsuse sensor第25頁/共34頁第二十五頁，共33頁。New technologies Reusable standard Single sensors use Sensor optionalEz-Control第26頁/共34頁第二十六頁，共33頁。Small scale bioreactors第27頁/共34頁第二十七頁，共33頁。Small scale bioreactor system28第28頁/共34頁第二十八頁，共33頁。Small scale bioreactor Communication via web browser Software to do calibration/setpoints/ control loops on mult