番茄堿作用機制 - Medchemexpress - MCE中國

1、Product Data SheetTomatidineCat. No.: HY-N2149CAS No.: 77-59-8分式: CHNO分量: 415.65作靶點: NF-B; JNK作通路: NF-B; MAPK/ERK Pathway儲存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實驗 DMSO : 2.86 mg/mL (6.88 mM; Need ultrasonic)H2O : 0.1 mg/mL (insoluble)SolventMass1 mg 5 mg 10 mgCo

2、ncentration制備儲備液1 mM 2.4059 mL 12.0294 mL 24.0587 mL5 mM 0.4812 mL 2.4059 mL 4.8117 mL10 mM 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲備液的保存式和期限：-80C, 6 months; -20C, 1 month。-80C 儲存時，請在 6 個內(nèi)使，-20C 儲存時，請在 1 個內(nèi)使。體內(nèi)實驗請根據(jù)您的實驗動物和給藥式選擇適當(dāng)?shù)娜芙獍?。以下溶解案都請先按?In Vitro 式配制澄清的儲備液，再依次添加助溶劑：為保證實驗結(jié)果的可靠性，

3、澄 的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實驗的作液，建議您現(xiàn)現(xiàn)配，當(dāng)天使； 以下溶劑前顯的百分 指該溶劑在您配制終溶液中的體積占；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的式助溶1. 請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 0.29 mg/mL (0.70 mM); Clear solution此案可獲得 0.29 mg/mL (0.70 mM，飽和度未知) 的澄清溶液。以 1 mL 作液為例，取 100 L 2.8999999 mg/mL 的澄清DMSO 儲備液加到 400 L PE

4、G300 中，混合均勻；向上述體系中加 50 L Tween-80，混合均勻；然后繼續(xù)加 450 L 理鹽定容 1 mL。2. 請依序添加每種溶劑： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 0.29 mg/mL (0.70 mM); Clear solutionPage 1 of 2 www.MedChemE此案可獲得 0.29 mg/mL (0.70 mM，飽和度未知) 的澄清溶液。以 1 mL 作液為例，取 100 L 2.8999999 mg/mL 的澄 DMSO 儲備液加到 900 L 20% 的 SBE-CD 理鹽溶液中，混合均勻

5、。3. 請依序添加每種溶劑： 10% DMSO 90% corn oilSolubility: 0.29 mg/mL (0.70 mM); Clear solution此案可獲得 0.29 mg/mL (0.70 mM，飽和度未知) 的澄 溶液，此案不適于實驗周 期在半個以上的實驗。以 1 mL 作液為例，取 100 L 2.8999999 mg/mL 的澄 DMSO 儲備液加到 900 L 油中，混合均勻。BIOLOGICAL ACTIVITY物活性 Tomatidine 通過阻斷 NF-B 和 JNK 信號發(fā)揮抗炎作。IC & Target p65 JNK體外研究 Tomatidine d

6、ecreases inducible NO synthase and COX-2 expression through suppression of I-B phosphorylation,NF-B nuclear translocation and JNK activation, which in turn inhibits c-jun phosphorylation and Oct-2 expression.Tomatidine, solasodine and diosgenin (40 M) show 66%, 22% and 41% inhibition of nitrite prod

7、uction, respectively.The iNOS protein is barely detectable in unstimulated cells but markedly increases after LPS treatment, andTomatidine causes dose-dependent inhibition of LPS-induced iNOS expression. p65 is the major component of NF-Bin LPS-stimulated macrophages, the effect of Tomatidine on p65

8、 DNA-binding activity is determined. In the presenceof Tomatidine at 10-40 M, the binding activity of NF-B is suppressed in a dose-dependent manner. Tomatidineinhibits the phosphorylation of I-B, blocks the I-B production, and furthermore suppresses p65 NF-B translocationto the nucleus and modulated

9、 binding activity1.PROTOCOLCell Assay 1 RAW 264.7 cells, derived from murine macrophages, are cultured in DMEM supplemented with 10% endotoxin-free,heat-inactivated fetal calf serum, Penicillin (100 units/mL), and Streptomycin (100 g/mL) in a 5% CO2 atmosphere at37C in a humidified incubator. For al

10、l assay, cell is plated at 2105 cells/cm2 in culture dishes or plates. Treatmentwith vehicle (0.1% DMSO or 0.1% ethanol), test compounds and/or LPS is carried out under serum-free conditions1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.戶使本產(chǎn)品發(fā)表的科研獻 F

11、ASEB J. 2019 Feb;33(2):2574-2586.See more customer validations on HYPERLINK www.MedChemE www.MedChemEREFERENCES1. Chiu FL, et al. Tomatidine inhibits iNOS and COX-2 through suppression of NF-kappaB and JNK pathways in LPS-stimulated mouse macrophages. FEBSLett. 2008 Jul 9;582(16):2407-12.McePdfHeightPage 2 of 3 www.MedChemECaution: Product has not been fully validate