常用(緩沖)溶液的配制

1、 # GuaranteedreagentGChemicalpureCP501藥品要有較好的質(zhì)量試劑分為優(yōu)級RAntalyticalreagentARLaboratoryreagentLR2藥品稱3配制試pH在5.57.0二）0.067（1/15）Mol/L磷酸緩沖液11/15Mol/L磷酸二KH2PO4，AR）9.08g1000ml容量瓶內(nèi)1000ml）。2/15Mol/LNa2HPO4A.R.9.47gNa2HPO42H2O11.87g入1000ml容量瓶內(nèi)，（1000ml）。3pH表1磷酸鹽緩沖液1/15Mol/L1/15Mol/LpH1/15Mol/L1/15Mol/LpHNa2HPO4

2、KH2PO4Na2HPO4KH2PO45.88.092.07.166.623.45.99.990.17.272.028.06.012.287.87.376.823.26.115.384.77.480.819.26.218.681.47.584.115.96.322.477.67.687.013.06.426.773.37.789.410.66.531.868.27.891.58.56.637.562.57.993.26.86.743.556.58.094.75.36.849.650.48.195.84.26.955.444.68.297.03.07.061.138.98.498.02.00.1

3、5Mol/LP表20.15Mol/LPpH0.15Mol/LNa2HPO4ml)0.15Mol/LNaH2PO4(ml)6.426.573.56.637.562.56.849.051.07.061.039.07.272.028.07.481.019.07.687.013.0Na2HPO42H2O量=175.050.15Mol/L含26.7g/LNa2HPO412H2O子量=358.220.15Mol/L含53.7g/LNaH2PO4H2O分子量=138.000.15Mol/L含20.7g/LNaH2PO42H2O子量=156.030.15Mol/L含23.4g/L0.2Mol/LP30.2mo

4、l/LPpH0.2Mol/LNa2HPO4(ml)0.2Mol/LNaH2PO4(ml5.88.092.06.012.387.76.218.581.56.426.573.56.637.562.56.849.051.07.061.039.07.272.028.07.481.019.07.687.013.07.891.58.58.094.75.30.2Mol/LNa2HPO42H2O35.61g/LNa2HPO412H2O71.64g/L0.2Mol/LNaH2PO4H2O27.60g/LNaH2PO42H2O31.21g/L0.1Mol/LPB0.2Mol/LPBH2O稀釋1PBS0.85的Na

5、ClPBS)0.15Mol/LPH7.2NaClKClNa2HPO4Na2HPO412H2OKH2PO4將上述試劑依次溶于1000ml10min15min48.0g0.2g1.15g2.89g)0.2g115Boaalin0.2Mol/L硼酸：硼酸12.37g水至1000ml。2Na2B4O70.05Mol/L硼砂：硼砂19.07g加水至1000ml。附表4pH0.2Mol/L硼酸pH0.05Mol/L硼砂0.2Mol/L硼酸pH0.05Mol/L硼砂0.2Mol/L（ml）（ml）ml)（ml）7.41.09.08.23.56.57.61.58.58.44.55.57.82.08.08.76

6、.04.08.03.07.09.08.02.013BO31pH8.2離子強度0.05Mol/L巴比妥巴比妥鈉47.6g蒸餾水3000ml1.17NHCl55.0ml1.17NHCl=193ml濃HCl加1807ml4.76g巴比妥鈉溶于3000ml加1.17NHCl55ml。用HClpH8.2加蒸餾水至4265ml2pH8.40.06Mol/L巴比妥1.84g10.30g1000.00ml3pH8.6O.O6M0I/L1.66g比妥鈉12.76g蒸餾水1000.00ml0.2Mol/L醋酸鹽緩沖液見表附表5附表50.2Mol/L醋酸鹽緩pH0.2M0l/L0.1M0l/LpH0.2M0l/L0

7、.1M0l/L2337Tris(ml)HCl(ml)2337Tris(ml)HCl(ml)9.108.952558.067.902527.58.928.76257.57.967.822530.08.758.602510.57.877.732532.58.628.482512.57.777.632535.08.508.372515.57.667.522537.58.408.272517.57.547.402540.08.328.182520.07.367.222542.5TrisHClpH0.05M0l/L25ml0.2M0l/L三羥甲基mlO.lNHCl100ml表6TrisHClpH0.2M

8、0l/L0.1NHClpH0.2M0l/L0.1NHCl2337Tris(ml)(ml)2337Tris(ml)(ml)9.108.962558.057.902527.58.928.78257.57.967.822530.08.748.602510.07.877.732532.58.628.482512.57.777.632535.08.508.372515.07.667.522537.58.408.272517.57.547.402540.08.328.182520.07.367.222542.58.238.102522.57.207.052545.08.148.002525.025Tris

9、=121.140.2Mol/LTris24.23g水至1000ml0.1NHCl：HC18.6ml水至1000ml。0.5Mol/LpH9.5NaHCO33.70gNa2CO30.60g于600.00ml蒸餾水中即得。用時塞緊瓶塞，以免吸收空氣中二氧化碳使PH1pH8.094.50ml5.50ml0.1Mol/LNa2HPO40.1NNaH2PO429份甘油與1份pH8.0Hans1貯存液NaCl80.00gKCl4.00gNa2HPO412H2O1.52gKH2PO40.60g4.00g0.4酚紅液50.00ml100.00ml11515min11515min臨用時用滅菌的5.6NaHCO3

10、液調(diào)pH值至7.2左右(Nesslsreage)nt)方1：氯化汞6.0g12.4g20NaOH30ml100ml方2：碘化汞11.5g8g50ml，加20NaOH50ml。l2.05g0.80g0.42g100ml溶解后，以10%檸檬酸調(diào)節(jié)pH至6.1，過濾，分裝，1010min440萬U)5200ml滅g/ml100U/ml200g1100ml-10-20臨用時，按1%的量加入營養(yǎng)液100ug/ml。150g300ml3000ml 2：濃硫酸50%蒸餾水50%重鉻酸鉀5%3：重鉻酸鉀100g蒸餾水1000ml100ml12h不予喂飼，僅予水喝。采血時采用頸凈滅菌。將采血筒中放入少量Hank

11、sHanks134h 2000r/minlOmin15min1500ml3500ml5630min用的血清可置435附表7常用化合物的分子量及當(dāng)量HCl36.4636.46NaOH40.0040.00KOH56.1156.11H2C2O490.0445.02NaCl58.4458.44KCl74.5674.56H2SO498.0849.04CH3COOH60.0560.05H3PO498.0032.67NaH2PO4119.98Na2HPO4141.96KH2PO4136.09K2HPO4174.18C10H14N2O8Na2372.24186.12EDTANa21 # HNO363.0163

12、.01I2253.81126.90KI166.01166.01H3BO361.8320.61NaHCO384.0184.01Na2CO3105.9953.00Na2B4O7381.43用1L(1000mlM表示。物質(zhì)氯化鈉的分子量為58.44，則1個克分子氯化鈉的58.44g58.44g氯化鈉溶解于水，配制成1L溶液，就是1Mol/L氯化NaH2PO4H2O的分子量為138.0,則其1個克分子的重量是138.069.0gNaH2PO4H2O于水，配制成1L溶液，就是0.5Mol/L4，000，每分鐘20，00020，000以上的離心RelativeCentrifugalForce，RCF1 G

13、ravityG=9.8米/秒2一()2r葉9802(亍/0.1047260/0.104722(F)二980cm30=|2520匚19一18一17-16一15-14-13一12一1110-9-8-7-654-3一70006000500040003000200015001000-800700600500TT40030020015010070-60一504030201510700.000600.000500.000400.000300.000200.000150.000100.00080.00070.00060.00050.00040.00030.00020.00015.00010.0008.0007.0006.0005.0004.0003.0002.0001.5001.000RPM5000至4500=4000=3500三3000=2500=2000二15