Hydrocortisone-Cortisol-DataSheet-生命科學(xué)試劑-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEHydrocortisoneCat. No.: HY-N0583CAS No.: 50-23-7Synonyms: Cortisol分式: CHO分量: 362.46作靶點(diǎn): Glucocorticoid Receptor; Endogenous Metabolite作通路: GPCR/G Protein; Metabolic Enzyme/Protease儲存式: 4C, protect from light* In solvent : -80C,

2、6 months; -20C, 1 month (protect fromlight)溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 31 mg/mL (85.53 mM)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制備儲備液1 mM 2.7589 mL 13.7946 mL 27.5893 mL5 mM 0.5518 mL 2.7589 mL 5.5179 mL10 mM 0.2759 mL 1.3795 mL 2.7589 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并

3、請注意儲備液的保存式和期限。體內(nèi)實(shí)驗(yàn)請根據(jù)您的實(shí)驗(yàn)動物和給藥式選擇適當(dāng)?shù)娜芙獍福渲魄罢埾扰渲瞥吻宓膬湟?，再依次添加助溶?為保證實(shí)驗(yàn)結(jié)果的可靠性，體內(nèi)實(shí)驗(yàn)的作液，建議您現(xiàn)現(xiàn)配，當(dāng)天使；澄清的儲備液可以根據(jù)儲存條件，適當(dāng)保存；以下溶劑前的百分 指該溶劑在您配制終溶液中的體積占)：1. 請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (6.90 mM); Clear solution2. 請依序添加每種溶劑： 10% DMSO 90% (20% SBE-CD in saline)Solubil

4、ity: 2.5 mg/mL (6.90 mM); Clear solution3. 請依序添加每種溶劑： 10% DMSO 90% corn oil1/3 Master of Small Molecules 您邊的抑制劑師www.MedChemESolubility: 2.5 mg/mL (6.90 mM); Clear solutionBIOLOGICAL ACTIVITY物活性 Hydrocortisone腎上腺質(zhì)分泌的類 醇激素或糖質(zhì)激素。IC50 & Target Human Endogenous Metabolite體外研究 Hydrocortisone (50 nM) shows

5、 a dose-dependent down-regulation of GR transcript in hCMEC/D3 cells.Hydrocortisone supplementation of the serum-reduced cell differentiation medium leads to a significantincrease in TER across the hCMEC/D3 monolayer 1. Hydrocortisone-treated Dendritic cells (DCs) show adecreased expression of MHC I

6、I molecules, the costimulatory molecule CD86, and the DC-specific markerCD83, as well as a strongly reduced IL-12 secretion. Hydrocortisone-treated DCs inhibit production of IFN-but induce an increased release of IL-4 and no change in IL-5 2. Hydrocortisone reduces postischemicoxidative stress, perf

7、usion pressure, and transudate formation. Hydrocortisone inhibits postischemicshedding of syndecan-1, heparan sulfate, and hyaluronan as is release of histamine from resident mast cells3.PROTOCOLCell Assay 1 Cells are plated on top of collagen IV-coated transwell chambers for six-well plates (24 mm

8、diameter,membrane material: polyethylene terephthalate (PET), 0.4 m pores, pore density 1.6106 cm2) at densitiesof 2.5104 cells cm2 per well. When they have reached confluence at day 5, the different experimental setsof cells are transferred to differentiation medium containing reduced amounts of FC

9、S and treated with TNFor hydrocortisone as indicated.MCE has not independently confirmed the accuracy of these methods. They are for reference only.戶使本產(chǎn)品發(fā)表的科研獻(xiàn) Cancer Manag Res. 2019 Jul. Cancer Manag Res. 2019 Jun 21;11:5557-5572.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1.

10、 F?rster C, et al. Differential effects of hydrocortisone and TNFalpha on tight junction proteins in an in vitro model of the human blood-brain barrier. J Physiol. 2008 Apr 1;586(7):1937-49.2. Bellinghausen I, et al. Inhibition of human allergic T-cell responses by IL-10-treated dendritic cells: dif

11、ferences from hydrocortisone-treated dendritic cells. J Allergy Clin Immunol. 2001 Aug;108(2):242-9.3. Chappell D, et al. Hydrocortisone preserves the vascular barrier by protecting the endothelial glycocalyx. Anesthesiology. 2007Nov;107(5):776-84.2/3 Master of Small Molecules 您邊的抑制劑師www.MedChemEMcePdfHeightCaution: Product has not