CU-CPT17e - Toll-like Receptor (TLR) Agonist - 生命科學試劑 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemECU-CPT17eCat. No.: HY-101929CAS No.: 2109805-75-4分式: CHNO分量: 504.49作靶點: Toll-like Receptor (TLR)作通路: Immunology/Inflammation儲存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實驗 DMSO : 5 mg/mL (9.91 mM;

2、 Need ultrasonic and warming)Mass Solvent1 mg 5 mg 10 mg Concentration制備儲備液1 mM 1.9822 mL 9.9110 mL 19.8220 mL5 mM 0.3964 mL 1.9822 mL 3.9644 mL10 mM - - -請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存式和期限。BIOLOGICAL ACTIVITY物活性 CU-CPT17e種多 Toll 樣受體 (TLR) 激動劑，可激活 TLR3，TLR8 和 TLR9。IC50 & Target TLR3 TLR8 TL

3、R9體外研究CU-CPT17e shows strong NF-B activation in TLR3, TLR8 and TLR9 HEK293 cells with EC50 values of4.800.73, 13.50.58 and 5.660.17 M, respectively. CU-CPT17e significantly improves the activity with13.90.9 fold of NF-B activation and an EC50 value of 4.80.7 M. CU-CPT17e inhibits the proliferation o

4、f1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemEHeLa cancer cells by triggering apoptosis and arresting the cell cycle at the S phase. The induction ofapoptosis by CU-CPT17e in HeLa cells is investigated. HeLa cells are cultured with increasingconcentrations of CU-CPT17e or poly I:C or blank contr

5、ol (DMSO) for 24 h. Treatment with CU-CPT17e for24 h at different concentrations (10 to 40 M) results in an elevation of apoptotic cell population ranging from10% to 17%, which is more effective than poly I:C at 5 g/mL. These results suggest that the antiproliferativeactivity of CU-CPT17e against He

6、La cells might result from its ability to directly induce apoptosis 1.PROTOCOLCell Assay 1 HeLa cells are seeded at a density of 3105 cells/well in 6-well plates and allowed to attach for 24 h. Aftertreatment of indicated concentrations of CU-CPT17e or poly I:C (5 g/mL) for another 24 h, cells areha

7、rvested with 0.25% trypsin without EDTA and rinsed twice with PBS, then stained using a Annexin V-FITCapoptosis detection kit. Cells are analyzed with a BD Accuri C6 flow cytometer 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Zhang L, et al. Discovery of Small Molecules as Multi-Toll-like Receptor Agonists with Proinflammatory and Anticancer Activities. J MedChem. 2017 Jun 22;60(12):5029-5044.McePdfHeightCaution: Product has not been fully validated for medical applications.For re