分析化學(xué)1光盤(pán)2

1、Chromatography is anytical methodt is widely used for theseparation, identification and determination of the chemical components incomplex mixtures. No other separation method is asapplicable as chromatography.erful and generallyChromatography was invented by Russian botanist Mikhail Tstheturn of th

2、e 20 century. Various plant pigments were loaded on the glass columns packed with calcium carbonate, then separated and eluted by petroleum ether. The separated species appeared as colored bands on the column, which accounts for the name of the method (Greek chroma meaning color and graphein meaning

3、 to write). Up to now, chromatography is difficult to define rigorously because the term has been app d to such a variety of systems and techniques. However, all of these methods have a common placehe use of the s ionary phase and the mobile phase.The sionary phase in chromatography is the phase whi

4、ch is fixed inplace either in a column or on a planar surface. The mobile phase is the phasewhich moves through the sionary phase, carrying theyte with it.Components of a mixture are loaded through the sionary phase and elutedby the flow gaseous or liquid mobile phase. Finally, separation occurs bec

5、ausesle components have different affinities betn the sionary and mobilephase, and they have different migrant rates and different retention times.第十六章 色譜分析法概論chromatography 色譜法sionary phase 固定相mobile phase elution 洗脫chromatogram 色譜圖baseline 基線 peak 色譜峰 tailing peak 拖尾峰leading peak 前延峰symmetry facto

6、r 對(duì)稱(chēng)因子相tailing factor 拖尾因子 retention time 保留時(shí)間 dead time 死時(shí)間adjusted retention time 調(diào)整保留時(shí)間retention volume 保留體積dead volume 死體積adjusted retention volume 調(diào)整保留體積relative retention 相對(duì)保留值 retention index 保留指數(shù) Kovats index; Kovats 指數(shù) peak height 峰高peak area 峰面積standard deviation 標(biāo)準(zhǔn)差peak widpeak width 峰寬lf

7、 height 半峰寬resolution 分離度distribution coefficient 分配系數(shù) partition coefficient 分配系數(shù) retention factor 保留因子gas chromatography ; GC 氣相色譜法liquid chromatography ; LC 液相色譜法supercritical fluid chromatography ; SFC 超臨界流體色譜法 bonded-phase chromatography ; BPC 鍵合相色譜法 column chromatography 柱色譜法planar chromatograp

8、hy ; plane chromatography 平面色譜法capillary electrophoresis ; CE 毛細(xì)管電泳法packed column 填充柱capillary columnmicrobore packed column 微填充柱open tubular column 開(kāi)管柱high performance liquid chromatography ; HPLC 高效液相色譜法pr chromatography 紙色譜法thin layer chromatography ; TLC 薄層色譜法thin film chromatography 薄膜色譜法partit

9、ion chromatography 分配色譜法partition coefficient 分配系數(shù)normal phase chromatography 正相色譜法 reversed phase chromatography 反相色譜法 adsorption chromatography 吸附色譜法 adsorption coefficient; Ka 吸附系數(shù)solvent strength; o 溶劑強(qiáng)度ion exchange chromatography ; IEC 離子交換色譜法ion exchanger 離子交換劑resin 樹(shù)脂molecular exclusteric exc

10、luchromatography; MEC 分子排阻色譜法chromatography ; SEC 空間排阻色譜法gel chromatography 凝膠色譜法gel permeation chromatography ;GPC 凝膠滲透色譜法 gel filtration chromatography ;GFC 凝膠過(guò)濾色譜法 permeation coefficient 滲透系數(shù)plate theory 塔板理論 rate theory 速率理論 number of plates 塔板數(shù)plate height (height equivalent to a theoretical plate); H 塔板高度effective number of plates; neff 有效板數(shù)effective plate height; Heff 有效塔板高度eddy diffu渦流擴(kuò)散packing factor 填充因子longit