常用熒光染料探針列表

常用熒光染料探針列表常用熒光染料探針列表常用熒光染料探針列表資料僅供參考文件編號(hào)：2022年4月常用熒光染料探針列表版本號(hào)：A修改號(hào)：1頁(yè)次：1.0審核：批準(zhǔn):發(fā)布日期：

這是來(lái)自于Salk的一個(gè)比較全的熒光染料列表，這些熒光染料可廣泛用于流式細(xì)胞術(shù)以及熒光顯微鏡技術(shù)，匯集了各種熒光染料的特性，方便大家查找?？筛鶕?jù)實(shí)際所用的檢測(cè)平臺(tái)、染料的最大激發(fā)光波長(zhǎng)和最大發(fā)射光波長(zhǎng)來(lái)選擇合適的熒光染料用于實(shí)驗(yàn)。請(qǐng)注意這上面所顯示的顏色可能會(huì)由于所用瀏覽器不同而有所不同，他們只是一個(gè)與實(shí)際顏色的近似值。染料名稱Excitation(nm)Emission(nm)分子量備注信息ReactiveandconjugatedprobesHydroxycoumarin325386331SuccinimidylesterAminocoumarin350445330SuccinimidylesterMethoxycoumarin360410317SuccinimidylesterCascadeBlue(375);401423596HydrazidePacificBlue403455406MaleimidePacificOrange403551

Luciferyellow425528

NBD466539294NBD-XR-Phycoerythrin(PE)480;565578240k

PE-Cy5conjugates480;565;650670

akaCychrome,R670,Tri-Color,QuantumRedPE-Cy7conjugates480;565;743767

Red613480;565613

PE-TexasRedPerCP490675

PeridininchlorphyllproteinTruRed490,675695

conjugateFluorX494520587GEHealthcareFluorescein495519389FITC;pHsensitiveBODIPY-FL503512

TRITC547572444TRITCX-Rhodamine570576548XRITCLissamineRhodamineB570590

TexasRed589615625SulfonylchlorideAllophycocyanin(APC)650660104k

APC-Cy7conjugates650;755767

PharRedAlexaFluor系列熒光染料AlexaFluor350343442410

AlexaFluor4054014211028

AlexaFluor430434540702

AlexaFluor488499519643QYAlexaFluor500503525700

AlexaFluor514517542714

AlexaFluor532530555724QYAlexaFluor5465615721079QYAlexaFluor5555535681250QYAlexaFluor568579603792QYAlexaFluor594591618820QYAlexaFluor6106106291285

AlexaFluor6336326481200

AlexaFluor6476526681300QYAlexaFluor6606636911100

AlexaFluor6806807021150

AlexaFluor7006967191400

AlexaFluor7507527761300

AlexaFluor7907828041750

Cy系列熒光染料Cy2489506714QYCy3(512);550570;(615)767QYCy3B558572;(620)658QY581594;(640)1102QYCy5(625);650670792QY6756941128QYCy7743767818QY核酸相關(guān)探針染料Hoechst33342343483616AT-selectiveDAPI345455

AT-selectiveHoechst33258345478624AT-selectiveSYTOXBlue431480~400DNAChromomycinA3445575

CG-selectiveMithramycin445575

YOYO/p>

EthidiumBromide493620394

AcridineOrange503530/640

DNA/RNASYTOXGreen504523~600DNATOTO-1,TO-PRO-1509533

Vitalstain,TOTO:CyanineDimerTO-PRO:CyanineMonomerThiazoleOrange510530

PropidiumIodide(PI)536617

LDS751543;590712;607472DNA(543ex/712em),RNA(590ex/607em)7-AAD546647

7-aminoactinomycinD,CG-selectiveSYTOXOrange547570~500DNATOTO-3,TO-PRO-3642661

DRAQ5647681,697413usableexcitationdownto488細(xì)胞功能探針I(yè)ndo-1361/330490/4051010AMester.Low/HighCa++,Fluo-3506526855AMester.pH>6DCFH5055355292'7'Dichorodihydrofluorescein,oxidizedformDHR505534346Dihydrorhodamine123,oxidizedform,lightcatalyzesoxidationSNARF548/579587/635

pH6/9熒光蛋白

QYBRPS

Y66H360442

Y66F360508

EBFP380440

monomerEBFP2383448

20

monomerAzurite383447

15

monomerGFPuv385508

T-Sapphire399511

2625weakdimerCerulean433475

2736weakdimermCFP433475

1364monomerECFP434477

3

CyPet435477

1859weakdimerY66W436485

mKeima-Red440620

3

monomerTagCFP458480

29

dimerAmCyan1458489

29

tetramermTFP1462492

54

dimerS65A471504

MidoriishiCyan472495

25

dimerWildTypeGFP396,47550826k

S65C479507

TurboGFP48250226k37

dimerTagGFP482505

34

monomerS65L484510

Emerald487509

39weakdimer;(Invitrogen)S65T488511

EGFP48850726k34174weakdimerAzamiGreen492505

41

monomerZsGreen1493505105k40

tetramerTagYFP508524

47

monomerEYFP51452726k5160weakdimerTopaz514527

57

monomerVenus515528

5315weakdimermCitrine516529

5949monomerYPet517530

8049weakdimerTurboYFP52553826k

dimerZsYellow1529539

13

tetramerKusabiraOrange548559

31

monomermOrange548562

499monomermKO548559

31122monomerTurboRFP55357426k62

dimertdTomato554581

9598tandemdimerTagRFP555584

50

monomerDsRedmonomer556586~28k16monomerDsRed2("RFP")563582~110k24

mStrawberry574596

2615monomerTurboFP60257460226k26

dimerAsRed2576592~110k13

tetramermRFP1584607~30k

monomerJ-Red584610

13dimermCherry587610

1696monomerHcRed1588618~52k

dimerKatusha588635

23

dimermKate(TagFP635)588635

15

monomerTurboFP63558863526k22

dimermPlum590649

53

mRaspberry598625

13

monomer;fasterphotobleachthanmPlum其他探針Monochlorobimane380461226GlutathioneprobeCalcein496517623pH>5

Ex:Peakexcitationwavelength(nm)Em:Peakemissionwavelength(nm)QY:QuantumyieldBR:Brightness;Extinctioncoefficient*Q