Berberine-chloride-hydrate-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?Agonists?ScreeningLibrarieswww.MedChemEBerberinechloridehydrateCat.No.:HY-17577CASNo.:68030-18-2分?式:C??H??ClNO?分?量:389.83作?靶點(diǎn):Topoisomerase;Autophagy;Bacterial;ReactiveOxygenSpecies;Antibiotic作?通路:CellCycle/DNADamage;Autophagy;Anti-infection;Immunology/Inflammation;MetabolicEnzyme/Protease;NF-κB儲(chǔ)存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:≥3.9mg/mL(10.00mM)掃描?維碼，H2O:1.25mg/mL(3.21mM;Needultrasonic)運(yùn)?溶解?案計(jì)算器*"≥"meanssoluble,butsaturationunknown.獲得適合您實(shí)驗(yàn)體系的溶解?案MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM2.5652mL12.8261mL25.6522mL5mM0.5130mL2.5652mL5.1304mL10mM0.2565mL1.2826mL2.5652mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲(chǔ)備液，并請注意儲(chǔ)備液的保存?式和期限。BIOLOGICALACTIVITY?物活性Berberinechloridehydrate(NaturalYellow18chloridehydrate)從中草藥連中分離出來的?種?物堿，常?作抗?素。Berberinechloridehydrate誘導(dǎo)活性氧(ROS)?成并抑制DNA拓?fù)洚悩?gòu)酶(topoisomerase)?？鼓[瘤特性[1]。IC50&TargetROS[1]DNAtopoisomerase[1]1/4www.MedChemEwww.MedChemE體外研究Berberinechloridehydrate(NaturalYellow18chloridehydrate;1.25-160μM;72hours)haspotentialinhibitoryeffectsontheproliferationoffourcolorectalcarcinomacelllinesLoVo,HCT116,SW480,andHT-29[1].Berberinechloridehydrate(1.25-160μM;24-72hours)inducesatime-anddose-dependentinhibitionofLoVocellgrowth[1].LoVocellsareexposuretoBerberinechloridehydrate(10-80μM)for24h.Cellcycleanalysisof40μMBerberine-treatedLoVocellsbyflowcytometryshowsaccumulationofcellsintheG2/Mphase[1].Berberinechloridehydrate(10-80μM)suppressescyclinB1,cdc2andcdc25cproteinexpressionafter24h,especiallyatthedoseof80.0μM[1].CellProliferationAssay[1]CellLine:FourcolorectalcarcinomacelllinesLoVo,HCT116,SW480,andHT-29Concentration:1.25,2.5,5,10,20,40,80,and160μMIncubationTime:72hoursResult:Inhibitedtheproliferationoffourcelllines.TheIC50rangedfrom40.8±4.1μM(LoVo)to98.6±2.9μM(HCT116).CellProliferationAssay[1]CellLine:ColorectalcarcinomacelllinesLoVoConcentration:1.25,2.5,5,10,20,40,80,and160μMIncubationTime:24,48,72hoursResult:Inducedatime-anddose-dependentinhibitionofcellgrowth.By72h,160.0μMinduced71.1±1.9%growthinhibitionsinLoVocells.CellCycleAnalysis[1]CellLine:LoVocellsConcentration:0,10,20,40,or80μMIncubationTime:24hoursResult:Exposureto40.0μMinducedG2/M-phasecellcyclearrest,anincreaseintheG2/M-phasepopulationandaprogressivedeclineintheG1population.WesternBlotAnalysis[1]CellLine:LoVocellsConcentration:10,20,40,or80μMIncubationTime:24hours2/4www.MedChemEwww.MedChemEResult:SuppressedcyclinB1,cdc2andcdc25cproteinexpression.體內(nèi)研究Berberinechloridehydrate(NaturalYellow18chloridehydrate;10,30,or50mg/kg/day;gastrointestinalgavage;for10consecutivedays)inhibitsthegrowthofhumancolorectaladenocarcinomainvivo.Berberinechloridehydrateatdosesof30and50mg/kg/daytakenbygastrointestinalgavageshowsinhibitoryratesof33.1%and45.3%onthehumancolorectaladenocarcinomaxenograftgrowthinnudemice[1].AnimalModel:5-week-oldBALB/cnu/numicewithhumancolorectaladenocarcinomaLoVoxenografts[1]Dosage:10,30,or50mg/kg/dayAdministration:Gastrointestinalgavage;for10consecutivedaysResult:Showedinhibitoryratesof33.1%and45.3%atdosesof30and50mg/kg/day.戶使?本產(chǎn)品發(fā)表的科研?獻(xiàn)?MechAgeingDev.2020Oct;191:111347.?ExpCellRes.2020Aug1;393(1):112054.?JApplMicrobiol.2021Apr;130(4):1154-1172.?JBoneMinerMetab.2021Apr8.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].CaiY,etal.Berberineinhibitsthegrowthofhumancolorectaladenocarcinomainvitroandinvivo.JNatMed.2014Jan;68(1):53-62.McePdfHeight3/4www.MedChemEwww.MedChemE關(guān)注MCE中國公眾號(hào)，

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