流式細(xì)胞術(shù)原理和應(yīng)用簡介課件

流式細(xì)胞術(shù)原理與應(yīng)用簡介湖北中醫(yī)學(xué)院中醫(yī)藥實(shí)驗(yàn)中心田代志流式細(xì)胞術(shù)1流式細(xì)胞術(shù)發(fā)展史1930年Caspersson和THorel開始致力于細(xì)胞計(jì)數(shù)的研究1934年Moldaven最早設(shè)想細(xì)胞檢測自動(dòng)化,用光電儀記錄流過一根毛細(xì)管的細(xì)胞1940年Coons提出結(jié)合熒光素的抗體去標(biāo)記細(xì)胞內(nèi)的特定蛋白1949年WallaceCoulter申請了在懸液中計(jì)數(shù)粒子的方法的專利1950年Caspersson用顯微UV-VIS檢測細(xì)胞流式細(xì)胞術(shù)發(fā)展史2流式細(xì)胞術(shù)發(fā)展史1953年Crosland-Taylor應(yīng)用分層蔚流原理,成功設(shè)計(jì)紅細(xì)胞光學(xué)自動(dòng)計(jì)數(shù)器1969年VanDilla及其同事在LosAlamos,NM發(fā)明第一臺(tái)熒光檢測細(xì)胞計(jì)1972年Herzenberg研制出細(xì)胞分選器1975年Kohler等提出單克隆抗體技術(shù),為細(xì)胞研究提供大量的特異免疫試劑大量廠家不斷生產(chǎn)流式細(xì)胞儀流式細(xì)胞術(shù)發(fā)展史3流式細(xì)胞術(shù)發(fā)展史目前國內(nèi)主要流式細(xì)胞儀廠家Becktondickinson(BD)公司BACKMANCOULTER公司流式細(xì)胞術(shù)發(fā)展史4流式細(xì)胞儀構(gòu)造流式細(xì)胞儀(FlowCytometer,FCM)的結(jié)構(gòu)分五部分:流動(dòng)室及液流驅(qū)動(dòng)系統(tǒng)激光光源及光束成形系統(tǒng)光學(xué)系統(tǒng)信號檢測與存貯、顯示、分析系統(tǒng)細(xì)胞分選及濃縮系統(tǒng)流式細(xì)胞術(shù)(FlowCytometry,FCM)圖示如下頁流式細(xì)胞儀構(gòu)造5流式細(xì)胞術(shù)原理和應(yīng)用簡介課件6流式細(xì)胞術(shù)原理和應(yīng)用簡介課件7流式細(xì)胞術(shù)原理和應(yīng)用簡介課件8流式細(xì)胞術(shù)原理和應(yīng)用簡介課件9流式細(xì)胞術(shù)原理和應(yīng)用簡介課件10流式細(xì)胞術(shù)原理和應(yīng)用簡介課件11流式細(xì)胞術(shù)原理和應(yīng)用簡介課件12流式細(xì)胞術(shù)原理和應(yīng)用簡介課件13流式細(xì)胞術(shù)原理和應(yīng)用簡介課件14流式細(xì)胞術(shù)原理和應(yīng)用簡介課件15流式細(xì)胞術(shù)原理和應(yīng)用簡介課件16流式細(xì)胞術(shù)原理和應(yīng)用簡介課件17流式細(xì)胞術(shù)原理和應(yīng)用簡介課件18流式細(xì)胞術(shù)原理和應(yīng)用簡介課件19流式細(xì)胞術(shù)原理和應(yīng)用簡介課件20流式細(xì)胞術(shù)原理和應(yīng)用簡介課件21流式細(xì)胞術(shù)原理和應(yīng)用簡介課件22流式細(xì)胞術(shù)原理和應(yīng)用簡介課件23流式細(xì)胞術(shù)原理和應(yīng)用簡介課件24流式細(xì)胞術(shù)原理和應(yīng)用簡介課件25流式細(xì)胞術(shù)原理和應(yīng)用簡介課件26流式細(xì)胞術(shù)原理和應(yīng)用簡介課件27流式細(xì)胞術(shù)原理和應(yīng)用簡介課件28流式細(xì)胞術(shù)原理和應(yīng)用簡介課件29流式細(xì)胞術(shù)原理和應(yīng)用簡介課件30流式細(xì)胞術(shù)原理和應(yīng)用簡介課件31流式細(xì)胞術(shù)原理和應(yīng)用簡介課件32流式細(xì)胞術(shù)原理和應(yīng)用簡介課件33流式細(xì)胞術(shù)原理和應(yīng)用簡介課件34流式細(xì)胞術(shù)原理和應(yīng)用簡介課件35流式細(xì)胞術(shù)原理和應(yīng)用簡介課件36流式細(xì)胞術(shù)原理和應(yīng)用簡介課件37流式細(xì)胞術(shù)原理和應(yīng)用簡介課件38流式細(xì)胞術(shù)原理和應(yīng)用簡介課件39流式細(xì)胞術(shù)原理和應(yīng)用簡介課件40流式細(xì)胞術(shù)原理和應(yīng)用簡介課件41流式細(xì)胞術(shù)原理和應(yīng)用簡介課件42流式細(xì)胞術(shù)原理和應(yīng)用簡介課件43流式細(xì)胞術(shù)原理和應(yīng)用簡介課件44流式細(xì)胞術(shù)原理和應(yīng)用簡介課件45流式細(xì)胞術(shù)原理和應(yīng)用簡介課件46流式細(xì)胞術(shù)原理和應(yīng)用簡介課件47流式細(xì)胞術(shù)原理和應(yīng)用簡介課件48流式細(xì)胞術(shù)原理和應(yīng)用簡介課件49流式細(xì)胞術(shù)原理和應(yīng)用簡介課件50流式細(xì)胞術(shù)原理和應(yīng)用簡介課件51流式細(xì)胞術(shù)原理和應(yīng)用簡介課件52流式細(xì)胞術(shù)原理和應(yīng)用簡介課件53流式細(xì)胞術(shù)原理和應(yīng)用簡介課件54流式細(xì)胞術(shù)原理和應(yīng)用簡介課件55流式細(xì)胞術(shù)原理和應(yīng)用簡介課件56流式細(xì)胞術(shù)原理和應(yīng)用簡介課件57流式細(xì)胞術(shù)原理和應(yīng)用簡介課件58流式細(xì)胞術(shù)原理和應(yīng)用簡介課件59流式細(xì)胞術(shù)原理和應(yīng)用簡介課件60流式細(xì)胞術(shù)原理和應(yīng)用簡介課件61流式細(xì)胞術(shù)原理和應(yīng)用簡介課件62流式細(xì)胞術(shù)原理和應(yīng)用簡介課件63流式細(xì)胞術(shù)原理和應(yīng)用簡介課件64流式細(xì)胞術(shù)原理和應(yīng)用簡介課件65流式細(xì)胞術(shù)原理和應(yīng)用簡介課件66流式細(xì)胞術(shù)原理和應(yīng)用簡介課件67流式細(xì)胞術(shù)原理和應(yīng)用簡介課件68流式細(xì)胞術(shù)原理和應(yīng)用簡介課件69流式細(xì)胞術(shù)原理和應(yīng)用簡介課件70流式細(xì)胞術(shù)原理和應(yīng)用簡介課件71流式細(xì)胞術(shù)原理和應(yīng)用簡介課件72流式細(xì)胞術(shù)原理和應(yīng)用簡介課件73流式細(xì)胞術(shù)原理和應(yīng)用簡介課件74流式細(xì)胞術(shù)原理和應(yīng)用簡介課件75流式細(xì)胞術(shù)原理和應(yīng)用簡介課件76流式細(xì)胞術(shù)原理和應(yīng)用簡介課件77流式細(xì)胞術(shù)原理與應(yīng)用簡介湖北中醫(yī)學(xué)院中醫(yī)藥實(shí)驗(yàn)中心田代志流式細(xì)胞術(shù)78流式細(xì)胞術(shù)發(fā)展史1930年Caspersson和THorel開始致力于細(xì)胞計(jì)數(shù)的研究1934年Moldaven最早設(shè)想細(xì)胞檢測自動(dòng)化,用光電儀記錄流過一根毛細(xì)管的細(xì)胞1940年Coons提出結(jié)合熒光素的抗體去標(biāo)記細(xì)胞內(nèi)的特定蛋白1949年WallaceCoulter申請了在懸液中計(jì)數(shù)粒子的方法的專利1950年Caspersson用顯微UV-VIS檢測細(xì)胞流式細(xì)胞術(shù)發(fā)展史79流式細(xì)胞術(shù)發(fā)展史1953年Crosland-Taylor應(yīng)用分層蔚流原理,成功設(shè)計(jì)紅細(xì)胞光學(xué)自動(dòng)計(jì)數(shù)器1969年VanDilla及其同事在LosAlamos,NM發(fā)明第一臺(tái)熒光檢測細(xì)胞計(jì)1972年Herzenberg研制出細(xì)胞分選器1975年Kohler等提出單克隆抗體技術(shù),為細(xì)胞研究提供大量的特異免疫試劑大量廠家不斷生產(chǎn)流式細(xì)胞儀流式細(xì)胞術(shù)發(fā)展史80流式細(xì)胞術(shù)發(fā)展史目前國內(nèi)主要流式細(xì)胞儀廠家Becktondickinson(BD)公司BACKMANCOULTER公司流式細(xì)胞術(shù)發(fā)展史81流式細(xì)胞儀構(gòu)造流式細(xì)胞儀(FlowCytometer,FCM)的結(jié)構(gòu)分五部分:流動(dòng)室及液流驅(qū)動(dòng)系統(tǒng)激光光源及光束成形系統(tǒng)光學(xué)系統(tǒng)信號檢測與存貯、顯示、分析系統(tǒng)細(xì)胞分選及濃縮系統(tǒng)流式細(xì)胞術(shù)(FlowCytometry,FCM)圖示如下頁流式細(xì)胞儀構(gòu)造82流式細(xì)胞術(shù)原理和應(yīng)用簡介課件83流式細(xì)胞術(shù)原理和應(yīng)用簡介課件84流式細(xì)胞術(shù)原理和應(yīng)用簡介課件85流式細(xì)胞術(shù)原理和應(yīng)用簡介課件86流式細(xì)胞術(shù)原理和應(yīng)用簡介課件87流式細(xì)胞術(shù)原理和應(yīng)用簡介課件88流式細(xì)胞術(shù)原理和應(yīng)用簡介課件89流式細(xì)胞術(shù)原理和應(yīng)用簡介課件90流式細(xì)胞術(shù)原理和應(yīng)用簡介課件91流式細(xì)胞術(shù)原理和應(yīng)用簡介課件92流式細(xì)胞術(shù)原理和應(yīng)用簡介課件93流式細(xì)胞術(shù)原理和應(yīng)用簡介課件94流式細(xì)胞術(shù)原理和應(yīng)用簡介課件95流式細(xì)胞術(shù)原理和應(yīng)用簡介課件96流式細(xì)胞術(shù)原理和應(yīng)用簡介課件97流式細(xì)胞術(shù)原理和應(yīng)用簡介課件98流式細(xì)胞術(shù)原理和應(yīng)用簡介課件99流式細(xì)胞術(shù)原理和應(yīng)用簡介課件100流式細(xì)胞術(shù)原理和應(yīng)用簡介課件101流式細(xì)胞術(shù)原理和應(yīng)用簡介課件102流式細(xì)胞術(shù)原理和應(yīng)用簡介課件103流式細(xì)胞術(shù)原理和應(yīng)用簡介課件104流式細(xì)胞術(shù)原理和應(yīng)用簡介課件105流式細(xì)胞術(shù)原理和應(yīng)用簡介課件106流式細(xì)胞術(shù)原理和應(yīng)用簡介課件107流式細(xì)胞術(shù)原理和應(yīng)用簡介課件108流式細(xì)胞術(shù)原理和應(yīng)用簡介課件109流式細(xì)胞術(shù)原理和應(yīng)用簡介課件110流式細(xì)胞術(shù)原理和應(yīng)用簡介課件111流式細(xì)胞術(shù)原理和應(yīng)用簡介課件112流式細(xì)胞術(shù)原理和應(yīng)用簡介課件113流式細(xì)胞術(shù)原理和應(yīng)用簡介課件114流式細(xì)胞術(shù)原理和應(yīng)用簡介課件115流式細(xì)胞術(shù)原理和應(yīng)用簡介課件116流式細(xì)胞術(shù)原理和應(yīng)用簡介課件117流式細(xì)胞術(shù)原理和應(yīng)用簡介課件118流式細(xì)胞術(shù)原理和應(yīng)用簡介課件119流式細(xì)胞術(shù)原理和應(yīng)用簡介課件120流式細(xì)胞術(shù)原理和應(yīng)用簡介課件121流式細(xì)胞術(shù)原理和應(yīng)用簡介課件122流式細(xì)胞術(shù)原理和應(yīng)用簡介課件123流式細(xì)胞術(shù)原理和應(yīng)用簡介課件124流式細(xì)胞術(shù)原理和應(yīng)用簡介課件125流式細(xì)胞術(shù)原理和應(yīng)用簡介課件126流式細(xì)胞術(shù)原理和應(yīng)用簡介課件127流式細(xì)胞術(shù)原理和應(yīng)用簡介課件128流式細(xì)胞術(shù)原理和應(yīng)用簡介課件129流式細(xì)胞術(shù)原理和應(yīng)用簡介課件130流式細(xì)胞術(shù)原理和應(yīng)用簡介課件131流式細(xì)胞術(shù)原理和應(yīng)用簡介課件132流式細(xì)胞術(shù)原理和應(yīng)用簡介課件133流式細(xì)胞術(shù)原理和應(yīng)用簡介課件134流式細(xì)胞術(shù)原理和應(yīng)用簡介課件135流式細(xì)胞術(shù)原理和應(yīng)用簡介課件136流式細(xì)胞術(shù)原理和應(yīng)用簡介課件137流式細(xì)胞術(shù)原理和應(yīng)用簡介課件138流式細(xì)胞術(shù)原理和應(yīng)用簡介課件139流式細(xì)胞術(shù)原理和應(yīng)用簡介課件140流式細(xì)胞術(shù)原理和應(yīng)用簡介課件141流式細(xì)胞術(shù)原理和應(yīng)用簡介課件142流式細(xì)胞術(shù)原理和應(yīng)用簡介課件143流式細(xì)胞術(shù)原理和應(yīng)用簡