OUL35-NSC39047-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEOUL35Cat.No.:HY-123512CASNo.:6336-34-1Synonyms:NSC39047分?式:C??H??N?O?分?量:256.26作?靶點(diǎn):PARP作?通路:CellCycle/DNADamage;Epigenetics儲(chǔ)存?式:4°C,protectfromlight*Insolvent:-80°C,6months;-20°C,1month(protectfrom

light)溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:83.33mg/mL(325.18mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM3.9023mL19.5114mL39.0229mL5mM0.7805mL3.9023mL7.8046mL10mM0.3902mL1.9511mL3.9023mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；?旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存?式和期限：-80°C,6months;-20°C,1month(protectfromlight)。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?。體內(nèi)實(shí)驗(yàn)請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請(qǐng)先按照InVitro?式配制澄的儲(chǔ)備液，再依次添加助溶劑：(為保證實(shí)驗(yàn)結(jié)果的可靠性，澄的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的?式助溶)1.請(qǐng)依序添加每種溶劑：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.08mg/mL(8.12mM);Clearsolution1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE2.請(qǐng)依序添加每種溶劑：10%DMSO>>90%cornoilSolubility:≥2.08mg/mL(8.12mM);ClearsolutionBIOLOGICALACTIVITY?物活性O(shè)UL35(NSC39047)選擇性的ARTD10(PARP-10)抑制劑，其IC50值為329nM。IC50&TargetARTD10/PARP10329nM(IC50)體外研究OUL35rescuesHeLacellsfromARTD10-inducedcelldeathandsensitizescellstoDNAdamage.OUL35(3μM)bindstoendogenousARTD10inU2OScells[1].OUL35alsoinhibitsARTD8(23.4μM),ARTD4(22.6μM),andARTD15(4.17μM)butwithmodestpotency[1].OUL35makeshydrogenbondstotheamideandcarbonylofGly888andtotheside-chainhydroxylofSer927.OUL35stacksbetweenTyr919andTyr932,like3ABinthecrystalstructure[1].REFERENCES[1].HarikanthVenkannagari,etal.Small-MoleculeChemicalProbeRescuesCellsfromMono-ADP-RibosyltransferaseARTD10/PARP10-InducedApoptosisandSensitizesCancerCellstoDNADamage.CellChemBiol.2016Oct20;23(10):1251-1260.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400-8