TC11-DataSheet-生命科學試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemETC11Cat.No.:HY-129478CASNo.:100823-03-8分?式:C??H??N?O?分?量:322.4作?靶點:Caspase;Bcl-2Family;CDK作?通路:Apoptosis;CellCycle/DNADamage儲存?式:4°C,protectfromlight*Insolvent:-80°C,6months;-20°C,1month(protectfrom

light)溶解性數(shù)據(jù)體外實驗DMSO:50mg/mL(155.09mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲備液1mM3.1017mL15.5087mL31.0174mL5mM0.6203mL3.1017mL6.2035mL10mM0.3102mL1.5509mL3.1017mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；?旦配成溶液，請分裝保存，避免反復凍融造成的產(chǎn)品失效。儲備液的保存?式和期限：-80°C,6months;-20°C,1month(protectfromlight)。-80°C儲存時，請在6個?內使?，-20°C儲存時，請在1個?內使?。體內實驗請根據(jù)您的實驗動物和給藥?式選擇適當?shù)娜芙?案。以下溶解?案都請先按照InVitro?式配制澄的儲備液，再依次添加助溶劑：(為保證實驗結果的可靠性，澄的儲備液可以根據(jù)儲存條件，適當保存；體內實驗的?作液，建議您現(xiàn)?現(xiàn)配，當天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的?式助溶)1.請依序添加每種溶劑：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(7.75mM);Clearsolution1/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemEBIOLOGICALACTIVITY?物活性TC11?種MCL1降解劑和Caspase-9和CDK1激活劑。TC11作為苯酞酰亞胺衍?物在結構上與免疫調節(jié)藥物有關。在長時間有絲分裂阻滯期間，TC11誘導MCL1降解導致凋亡性死亡[1][2]。IC50&TargetCDK1Caspase-9MCL1體外研究TC11(0~30μM;24hours;KMS34cells)inducescelldeathinKMS34[1].TC11(5μM;0~48hours;KMS34cells)inducescelldeathoccursthroughanapoptoticpathwayanddownregulatesMCL1expression[1].TC11(5μM;24hours;KMS34cells)inducesMarrest[1].CellViabilityAssay[1]CellLine:KMS34cellsConcentration:0~30μMIncubationTime:24hoursResult:Inducedcelldeath.WesternBlotAnalysis[1]CellLine:KMS34cellsConcentration:5μMIncubationTime:0~48hoursResult:InducedcelldeathoccursthroughanapoptoticpathwayanddownregulatedMCL1expression.CellCycleAnalysis[1]CellLine:KMS34cellsConcentration:5μMIncubationTime:24hoursResult:InducedMarrest.REFERENCES[1].IchikawaD,etal.Aphenylphthalimidederivative,TC11,inducesapoptosisbydegradingMCL1inmultiplemyelomacells.BiochemBiophysResCommun.2020;521(1):252-258.[2].ShiheidoH,etal.Aphthalimidederivativethatinhibitscentrosomalclusteringiseffectiveonmultiplemyeloma.PLoSOne.2012;7(6):e38878.2/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemEMcePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchus