FM4-64-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEFM4-64Cat.No.:HY-103466CASNo.:162112-35-8分?式:C??H??Br?N?分?量:607.51作?靶點(diǎn):Others作?通路:Others儲存?式:-20°C,sealedstorage,awayfrommoisture*Insolvent:-80°C,6months;-20°C,1month(sealed

storage,awayfrommoisture)溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:50mg/mL(82.30mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲備液1mM1.6461mL8.2303mL16.4606mL5mM0.3292mL1.6461mL3.2921mL10mM0.1646mL0.8230mL1.6461mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；?旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲備液的保存?式和期限：-80°C,6months;-20°C,1month(sealedstorage,awayfrommoisture)。-80°C儲存時(shí)，請?jiān)?個(gè)?內(nèi)使?，-20°C儲存時(shí)，請?jiān)?個(gè)?內(nèi)使?。BIOLOGICALACTIVITY?物活性FM4-64?種膜選擇性紅?熒光染料，屬于苯?烯吡啶染料FM家族。FM4-64被?泛?于研究活體動物的內(nèi)吞和外吞、囊泡運(yùn)輸和細(xì)胞器組織。體外研究FM4-64readilytranslocatestothenuclearenvelope(NE)atphysiologicaltemperaturewhereitexhibitsenhancedfluorescencewhenexcitedat620-650nmincontrastto480-520nmexcitationintheendocyticpathwayandintheendoplasmicreticulum(ER).Invitrodataindicatedthatthedyerevealsanenrichmentof1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemEnegativelychargedlipids,presumablyduetolocalphospholipidsynthesis.Dual-excitationimagingofFM4-64inrelationtolamina-associatedpolypeptide-1-greenfluorescentproteinduringmitosissuggestedthatthedisassemblyofNEpreservesmicroscalelipidcomplexesintheER[1].Forstudiesonplantandfungalcells,FM4-64isusuallypreferredtoFM1-43duetoitssuperiorbrightness,greatercontrast,higherphotostability,anditsred-shiftedemissionfacilitatingconcomitantusewiththegreenfluorescentprotein(GFP).[3]REFERENCES[1].ZalT,etal.SpectralshiftoffluorescentdyeFM4-64revealsdistinctmicroenvironmentofnuclearenvelopeinlivingcells.Traffic.2006;7(12):1607-1613.[2].StenovecM,etal.DistinctlabellingoffusioneventsinratlactotrophsbyFM1-43andFM4-64isassociatedwithconformationaldifferences.ActaPhysiol(Oxf).2007;191(1):35-42.[3].BolteS,etal,Satiat-JeunemaitreB.FM-dyesasexperimentalprobesfordissectingvesicletraffickinginlivingplantcells.JMicrosc.2004;214(Pt2):159-173.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuse