ML329-DataSheet-生命科學試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEML329Cat.No.:HY-101464CASNo.:19992-50-8分?式:C??H??N?O?S分?量:328.34作?靶點:Others作?通路:Others儲存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實驗DMSO:5mg/mL(15.23mM;Needultrasonic)DMF:5mg/mL(15.23mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲備液1mM3.0456mL15.2281mL30.4562mL5mM0.6091mL3.0456mL6.0912mL10mM0.3046mL1.5228mL3.0456mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；?旦配成溶液，請分裝保存，避免反復凍融造成的產(chǎn)品失效。儲備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲存時，請在6個?內使?，-20°C儲存時，請在1個?內使?。體內實驗請根據(jù)您的實驗動物和給藥?式選擇適當?shù)娜芙?案。以下溶解?案都請先按照InVitro?式配制澄的儲備液，再依次添加助溶劑：(為保證實驗結果的可靠性，澄的儲備液可以根據(jù)儲存條件，適當保存；體內實驗的?作液，建議您現(xiàn)?現(xiàn)配，當天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的?式助溶)1.請依序添加每種溶劑：0.5%CMC-Na/salinewater1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemESolubility:5mg/mL(15.23mM);Suspendedsolution;NeedultrasonicBIOLOGICALACTIVITY?物活性ML329?種?眼畸形相關轉錄因?(MITF)抑制劑，ML329抑制TRPM-1啟動?活性，IC50值為1.2μM。IC50&TargetIC50:1.2μM(TRPM-1)[1]體外研究ML329inhibitstheexpressionofnumerousmicropthalmia-associatedtranscriptionfactor(MITF)targetgenesandblockstheproliferationofnumerouscelllinesthatrequireMITFforproliferation.ML329coulddirectlyorindirectlyinteractwithMITForcomponentsoftheMITFregulatorynetwork.Asatranscriptionfactorthatregulatescellcycleandpigmentation,interferenceofMITFwithML329willbeusefulincharacterizingthespecificrolesofMITFinmelanomaandvalidateblockadeofMITFfunctionasapotentialtreatmentofmelanoma.ML329showsspecificactivityagainsttheMITF-dependentcells,primarymelanocytesbutnoeffectontheviabilityinA375cells.ML329reducestheexpressionofmultipleMITFtargetgenes,includingpigment-relatedgenesandthecellcycleregulatorCDK2.Asatoolcompound,ML329willbeusefulinelucidatingtheroleofMITFinmelanocytelineagedevelopmentandinmelanomadiseaseprogression[1].戶使?本產(chǎn)品發(fā)表的科研?獻?NatCommun.2021Jan13;12(1):362.?BrJDermatol.2021Jan5.?ClinTranslMed.2022Jul;12(7):e961.?CancerBiolMed.2019Aug;16(3):498-513.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].FaloonPW,etal.ASmallMoleculeInhibitoroftheMITFMolecularPathway.ProbeReportsfromtheNIHMolecularLibrariesProgram[Internet].Bethesda(MD):NationalCenterforBiotechnologyInformation(US);2010-2012Dec13.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Fo