Calcein-Fluorexon-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemECalceinCat.No.:HY-D0040CASNo.:1461-15-0Synonyms:Fluorexon分?式:C??H??N?O??分?量:622.53作?靶點(diǎn):Others作?通路:Others儲(chǔ)存?式:4°C,protectfromlight*Insolvent:-80°C,6months;-20°C,1month(protectfrom

light)溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:≥100mg/mL(160.63mM)0.1MNaOH:10mg/mL(16.06mM;ultrasonicandadjustpHto11withNaOH)*"≥"meanssoluble,butsaturationunknown.MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM1.6063mL8.0317mL16.0635mL5mM0.3213mL1.6063mL3.2127mL10mM0.1606mL0.8032mL1.6063mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；?旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存?式和期限：-80°C,6months;-20°C,1month(protectfromlight)。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?。體內(nèi)實(shí)驗(yàn)請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請(qǐng)先按照InVitro?式配制澄的儲(chǔ)備液，再依次添加助溶劑：(為保證實(shí)驗(yàn)結(jié)果的可靠性，澄的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的?式助溶)1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE1.請(qǐng)依序添加每種溶劑：2%NaHCO3inddH2OSolubility:20mg/mL(32.13mM);Clearsolution;NeedultrasonicandadjustpHto6withNaHCO3BIOLOGICALACTIVITY?物活性Calcein?種熒光染料和?滅探針，可以作為脂囊泡滲漏的指?劑，也可作EDTA鈣離?滴定和熒光測(cè)定鈣離?的絡(luò)合指?劑。體外研究CalceinaccumulatesintracellularlyincoelomocytesincubatedincoelomicfluidorISO-EDTA.CoelomocytesincubatedinCFshowanincreaseinthecalceinfluorescenceintensitycomparedtothecontrol.PROTOCOLCellAssay[1]Coelomocytessuspensions(ISOandCF)areseparatelyincubatedwithcalcein-AM(excitationandemissionwavelength:496nmand516nm,respectively)atafinalconcentrationof200nM,during30min,at26°C.Calcein-AMisanonfluorescentABCtransportersubstratewhoseintracellularaccumulationisinverselyproportionaltoABCtransporteractivity.Intracellularesterasesconvertcalcein-AMintothefluorescentdyecalcein,whichisnotanABCtransportersubstrate,therebyaccumulatingthedyeinsidethecell.Therefore,ahighfluorescencesignalindicateslowABCtransporteractivitywhereasalowfluorescencesignalindicateshighactivity.Thefluorescenceofsamplesismeasuredbyflowcytometer.Theexperimentisrepeatedsixtimesinduplicates.MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.戶使?本產(chǎn)品發(fā)表的科研?獻(xiàn)?ActaPharmSinB.2020Sep;10(9):1694-1708.?Antioxidants(Basel).2021May18;10(5):798.?JEthnopharmacol.2021Apr24;270:113770.?DrugDesDevelTher.2022,16:3929-3946.?IntJAdvManufTechnol.17January2022.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].Marques-SantosLF,etal.ABCB1andABCC1-liketransportersinimmunesystemcellsfromseaurchinsEchinometralucunterandEchinusesculentusandoystersCrassostreagasarandCrassostreagigas.FishShellfishImmunol.2017Sep5;70:195-203.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedica