降鈣素基因相關(guān)肽介導(dǎo)運動預(yù)適應(yīng)對急性力竭大鼠心臟保護作用的研究

降鈣素基因相關(guān)肽介導(dǎo)運動預(yù)適應(yīng)對急性力竭大鼠心臟保護作用的研究摘要：

降鈣素基因相關(guān)肽（CGRP）是一種廣泛分布于人體的神經(jīng)及非神經(jīng)元源性多肽物質(zhì)，已被證實能夠通過多種途徑對宿主產(chǎn)生心血管保護作用。本研究旨在探究降鈣素基因相關(guān)肽介導(dǎo)的運動預(yù)適應(yīng)對急性力竭大鼠心臟保護作用的機制。實驗設(shè)計采用隨機分組的方法，將大鼠分為對照組、急性力竭組、運動預(yù)適應(yīng)組、運動預(yù)適應(yīng)+急性力竭組，分別進行不同處理。結(jié)果顯示，相較于對照組，急性力竭組的大鼠心肌細胞出現(xiàn)明顯損傷；而運動預(yù)適應(yīng)組的大鼠心肌細胞則明顯減輕了急性力竭對心肌細胞的損傷作用；運動預(yù)適應(yīng)+急性力竭組的大鼠，雖然心肌細胞受損，但其損傷程度也明顯小于急性力竭組。同時，運動預(yù)適應(yīng)組和運動預(yù)適應(yīng)+急性力竭組的CGRPmRNA和蛋白表達量均顯著高于對照組和急性力竭組。據(jù)此推測，CGRP介導(dǎo)的運動預(yù)適應(yīng)能夠通過上調(diào)CGRP表達，發(fā)揮心肌細胞保護作用，有望成為心肌保護的新策略。

關(guān)鍵詞：降鈣素基因相關(guān)肽；急性力竭；運動預(yù)適應(yīng)；心臟保護；CGRPmRNA；CGRP蛋白

Abstract：

Calcitoningene-relatedpeptide(CGRP)isaneuropeptidewidelydistributedinthehumanbody,andithasbeenfoundtoproducecardiovascularprotectionthroughvariouspathways.ThisstudyaimedtoexplorethemechanismofCGRP-mediatedexercisepre-adaptationinprotectingtheheartofratsfromacutefatigue.Theexperimentaldesignadoptedarandomgroupingmethod,whichdividedtheratsintothecontrolgroup,acutefatiguegroup,exercisepre-adaptationgroup,andexercisepre-adaptationplusacutefatiguegroupfordifferenttreatment.Theresultsshowedthatthemyocardialcellsoftheratsintheacutefatiguegroupweresignificantlydamagedcomparedwiththoseinthecontrolgroup,whilethemyocardialcellsoftheratsintheexercisepre-adaptationgroupweresignificantlyrelievedfromthedamagecausedbyacutefatigue.Thedamagetothemyocardialcellsoftheratsintheexercisepre-adaptationplusacutefatiguegroupwasalsosignificantlylessthanthatintheacutefatiguegroup.Atthesametime,themRNAandproteinexpressionlevelsofCGRPintheexercisepre-adaptationgroupandexercisepre-adaptationplusacutefatiguegroupweresignificantlyhigherthanthoseinthecontrolgroupandacutefatiguegroup.ItisspeculatedthattheCGRP-mediatedexercisepre-adaptationcanplayaprotectiveroleinmyocardialcellsbyup-regulatingCGRPexpression,whichisexpectedtobecomeanewstrategyformyocardialprotection.

Keywords:Calcitoningene-relatedpeptide;acutefatigue;exercisepre-adaptation;heartprotection;CGRPmRNA;CGRPproteinInconclusion,ourstudydemonstratedthatexercisepre-adaptationcanup-regulateCGRPexpressionintheheart,whichmayplayaprotectiveroleinmyocardialcellsagainstacutefatigue.OurfindingssuggestthatCGRP-mediatedexercisepre-adaptationcouldsetanewstrategyformyocardialprotection.However,furtherstudiesareneededtoexploretheunderlyingmechanismsofCGRP-mediatedmyocardialprotectionanditspotentialtherapeuticbenefitsFutureresearchcanfocusonexploringthespecificmechanismsbywhichCGRPexertsitsprotectiveeffectsonmyocardialcells.Forinstance,itwouldbeinterestingtoinvestigatewhetherCGRPisinvolvedintheregulationofmyocardialenergymetabolismandmitochondrialfunction.Additionally,itwouldbeworthwhiletoinvestigatetheoptimaltiminganddurationofexercisepre-adaptationnecessarytoobservetheprotectiveeffectsonmyocardialcells.

Moreover,itwouldbeintriguingtoinvestigatethepotentialtherapeuticbenefitsofCGRP-mediatedexercisepre-adaptationinthepreventionandtreatmentofcardiovasculardiseases,suchasmyocardialinfarctionandheartfailure.Thiscouldinvolveconductingclinicaltrialstoexploretheefficacyandsafetyofexercisetraininginindividualswithcardiovasculardiseases,assessingtheeffectsofexercisepre-adaptationonmyocardialfunction,andevaluatingthepotentialofCGRPasatherapeutictargetformyocardialprotection.

Inconclusion,thepresentstudyprovidesevidencethatexercisepre-adaptationcanup-regulateCGRPexpressionintheheart,whichmayplayaprotectiveroleinmyocardialcellsagainstacutefatigue.OurfindingssuggestthattheCGRP-mediatedexercisepre-adaptationcouldsetanewstrategyformyocardialprotection.FurtherresearchisneededtofullyelucidatethemechanismsunderlyingCGRP-mediatedmyocardialprotectionandtoexplorethepotentialofCGRPasatherapeutictargetincardiovasculardiseasesInadditiontopotentialtherapeuticbenefits,understandingtheroleofCGRPinexercisepre-adaptationandmyocardialprotectionhasimportantimplicationsforsportsmedicineandathletictraining.Tomaximizeperformanceandpreventinjury,athletesoftenengageinpre-seasontrainingorconditioningprogramstocreatephysiologicaladaptationsthatwillenhancetheirabilitiesduringcompetition.IfCGRP-mediatedexercisepre-adaptationcouldbeutilizedintheseprograms,itmayofferanovelapproachtooptimizingathleticperformancewhilesimultaneouslyprotectingagainstmuscularandcardiovascularfatigue.

Moreover,theconceptofexercisepre-adaptationandtheroleofCGRPinmyocardialprotectionmayhavesignificantimplicationsforindividualswithcardiovasculardiseases.Exercisehaslongbeenrecognizedasapowerfultoolformanagingandpreventingcardiovasculardisease,however,manyindividualswithcardiacissuesmaybehesitanttoengageinphysicalactivityforfearofexacerbatingtheircondition.IfCGRP-mediatedexercisepre-adaptationcouldbeharnessedasatherapeuticstrategy,itmayofferawaytosafelyandeffectivelypromotecardiovascularhealthinthispopulation.

Inconclusion,thestudyofCGRPanditsroleinexercisepre-adaptationandmyocardialprotectionrepresentsanexcitingavenueforfutureresearch.Byfurtherelucidatingth