PCR技術(shù)及其應(yīng)用

PCR技術(shù)在醫(yī)學(xué)中的應(yīng)用醫(yī)學(xué)分子細(xì)胞生物學(xué)研究方法PCR技術(shù)及其應(yīng)用WhatisPCRHowtoPCRWhydoPCRPCR技術(shù)及其應(yīng)用Developedin1983byKaryMullispolymerasechainreaction(PCR)1993年的諾貝爾化學(xué)獎PCR技術(shù)及其應(yīng)用AnimationofPCRPCR技術(shù)及其應(yīng)用PCRReagentsTemplateDNAPrimersdNTPDNAPolymeraseBufferMg2+PCR技術(shù)及其應(yīng)用QuestionsAnneallingDNApolymerasev.s.WhyprimersareessentialforDNApolymerase?PCR技術(shù)及其應(yīng)用InitializationstepDenaturationstepAnnealingstepElongationstepFinalelongationFinalholdPCRprocedure&stages20~40CyclesExponentialamplificationLevelingoffstagePlateauPCR技術(shù)及其應(yīng)用EvolutionofmachinesforPCR1234PCR技術(shù)及其應(yīng)用PCROptimizationContaminationHairpinsPolymeraseerrorsMagnesiumconcentrationNon-specificprimingPrimerdimersDeoxynucleotidesGC-richTemplateProofreadingDNAPolymerasePfu,KOD…DMSO,betaineRedesignPrimersHot-StartPolymerasePCR技術(shù)及其應(yīng)用PCROptimization:PrimerDesignSoftwaresPrimerpremier:e-PCR(NCBI)Oligo7:Primer3:CommandlinestyledesigntoolMulti-OligodesignforprobesorprimersSinglepairedprimersdesignforPCRAvoidnon-specificamplificationbyblastPCR技術(shù)及其應(yīng)用Primersforamplificationofhumanb-actingenomicDNAregionPCR技術(shù)及其應(yīng)用PrimerDesign:avoidfailureofamplification5’-primer:stem-loopstructurewithhighmeltingtemperaturePCR技術(shù)及其應(yīng)用Highdifferencebetweenproductandprimermeltingtemperatures.3'-endForwardPrimerdimer.PrimerDesign:avoidfailureofamplification3'-enddimerbetweentheForwardandReverseprimers.TerminalstabilityoftheForwardPrimeristoohigh.PCR技術(shù)及其應(yīng)用PrimerDesign:avoidfailureofamplificationPCR技術(shù)及其應(yīng)用PCRmethodsPCR技術(shù)及其應(yīng)用IsothermalPCRReverseTranscriptionPCR

FrequentlyusedPCRmethodsReal-TimePCR(quantitativePCR)Overlap-extensionPCR

NestedPCRPCR技術(shù)及其應(yīng)用FrequentlyusedPCRmethodsReverseTranscriptionPCR

PCR技術(shù)及其應(yīng)用Overlap-extensionPCR

FrequentlyusedPCRmethodsPCR技術(shù)及其應(yīng)用NestedPCR

FrequentlyusedPCRmethodsPCR技術(shù)及其應(yīng)用FrequentlyusedPCRmethodsReal-TimePCR(quantitativePCR)DetectfluorescenceforeachcycleQuantitaivebyamplificationcurveQuanlitycontrolPCR技術(shù)及其應(yīng)用Real-TimePCRdouble-strandedDNA-bindingdyePCR技術(shù)及其應(yīng)用Real-TimePCR(quantitativePCR)DetectfluorescenceforeachcyclePCR技術(shù)及其應(yīng)用Real-TimePCR(quantitativePCR)QuanlitycontrolbymeltingcurvePCR技術(shù)及其應(yīng)用FluorescentreporterprobeReal-TimePCRPCR技術(shù)及其應(yīng)用Real-TimePCRPathogen

DiagnosismicroRNA

Diagnosisheredopathia

DiagnosisPCR技術(shù)及其應(yīng)用Iso-ThermalPCRIso-ThermalFastSensitiveLAMP/RPAPCR技術(shù)及其應(yīng)用Loop-mediatedisothermalamplification(LAMP)PCR技術(shù)及其應(yīng)用RecombinasepolymeraseamplificationTwoprimersQuantitativableSensitiveLongPrimersPCR技術(shù)及其應(yīng)用ApplicationofPCRPCR技術(shù)及其應(yīng)用ApplicationofPCRSelectiveDNAisolationAmplificationandQuantificationPCRindiagnosisofdiseasesPCRinforensicsciencePCR技術(shù)及其應(yīng)用PCRinforensicsciencevariablenumberoftandemrepeatsMitochondriaSequencingShortTandemRepeatPCR技術(shù)及其應(yīng)用MitochondriaSequencingPCRinforensicscienceShortTandemRepeatvariablenumberoftandemrepeatsPCR技術(shù)及其應(yīng)用PCRinforensicscienceSexdiscernmentY-chromosomespecificDNAPCR技術(shù)及其應(yīng)用MedicalapplicationofPCRPCR技術(shù)及其應(yīng)用MedicalapplicationsGenetictestingTissuetypingTumortargettypingPCR技術(shù)及其應(yīng)用CellFreeDNAanalysisPCR技術(shù)及其應(yīng)用majorcurrenttechniquesforanalyzingfetalchromosomesPCR技術(shù)及其應(yīng)用PCRindiagnosisoffetalheredopathiaPCR技術(shù)及其應(yīng)用BabygenderbloodtestsPCR技術(shù)及其應(yīng)用FetalsexdiscernmentChorionicvillussamplingObstetricultrasonographyCell-freefetalDNAafterthe13thweekofpregnancyAccuracy:~90%riskofdamagetothefetusa