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Antennabetweenelectrodes
usingEAGProbewithElectricallyconductivegelAntenna
holderPROBE(Inputamplifier)AntennaMountingtheantennausingelectricallyconductivegelAntenna
holderABCExampleofElectroantennogram(EAG)RecordingIncreasingconcentrationofstimulusQualityoftheEAGsignalSignaltoNoiseratioSourcesofNOISEElectronic
noiseFILTERBasicEAGstimulationarrangementStimulussource(Pasteurpipette)preparation12345EAGsignalqualityStimulusdurationEffectoffiltersettingEAGDataevaluationEAGrecordingof21stimulationsDeclineofantennalsensitivityovertimeNormalizationofEAGresponsesRefRefRefRefRefRefEAGInthefieldMultipleantennaeCombinedwithAirsensorRECORDINGINTHEFIELD
usingaportableEAGrecordingdeviceAntennacompartmentAntennaholderGelAntennaGelPortableEAGsensorontransportcase11Pheromoneconcentrationmeasurementsin4greenhouses1234Pheromoneconcentrationinitiallyhigh,thendeclines,andincreasesagain.mayapriljunejulyaugseptoctnov12MULTIPLEANTENNAEAGRECORDINGMULTIPLE(4)ANTENNAPROBE4antennaeinparallel:INCREASEDSENSITIVITY(Signal-NoiseRatio)123413antennamicrothermistor
MULTIMODALSIGNALRECORDINGEAGcombinedwithairspeedsensor
EAGsignalAir
speed14GC-EADElectroAntennoDetectionGas-ChromatographyCOMBINEDWITHGASCHROMATOGRAPHY115CoupledGasChromatography-ElectroantennoDetection(GC-EAD)
GC-EADorGC-SSRGCtransferlineinsectprep.EAGFIDCOMBINEDWITHGASCHROMATOGRAPHY216
COMBINEDWITHGASCHROMATOGRAPHY3Typicalexample
FIDEAG17SSRSingleSensillumRecordingRECORDINGthroughDIRECTCONTACT:2.SingleSensillumRecording(SSR)AMPLIFIERActionpotentials('spikes')STIMULUSMicroelectrodemakescontactwithasinglesensillumMicroelectrodeReferenceelectrodeActionpotentials('spikes')aregeneratedinresponsetoanactivecompoundsinglesensillumAntenna10SingleSensillumRecordingINTHEFIELDusingaportabledeviceAntennalolfactoryneuronactivityAirvelocityLaboratorySSRsetupPortableSSRrecordingunit20cm18RecordingBehaviouralResponsesSIGNALDETECTION
SIGNALTRANSDUCTIONSTIMULUSSIGNAL
PROCESSINGSIGNAL
RESPONSE(POWERSUPPLY)BEHAVIOURAL
RESPONSEELECTRICALSIGNALACTIVITYSIGNALRAWorPROCESSEDSIGNAL?19RECORDINGbymeansofaRADARBEAMACTIVITYPATTERNRECORDING(Wholeinsectorbodyparts)20RECORDINGbymeansofaRADARBEAMTobaccoodourdetectionbyconditioned(‘programmed’)beeApplicationexample21RECORDINGusingVIDEOPROCESSING1.Locomotionrecordingandanalysis
VIDEOCAMERASIGNALPROCESSORCOMPUTERTrackAnalyserTESTARENA(4-WayOLFACTOMETER)22RECORDINGusingOPTICALDISPLACEMENTSENSORThe"servo-sphere"or"LocomotionCompensator"Two-dimensional"locomotioncompensator"(Kramer,1976)PositionofinsectisrecordedbyanopticalpositionsensorDisplacementofinsectisanalysedbyasignalprocessorCommandsignalsaresenttotheX-andY-motorsto"counter-act"thedisplacementoftheinsect.23RECORDINGusingVIDEOPROCESSING1Locomotionrecordingusingthe"servo-sphere"
videosensorsphere30cmHungryColoradopotatobeetlelookingforapotatoplant24RECORDINGusingVIDEOPROCESSING2Locomotionrecordingusingamini"servo-sphere",1
2.Ticknymph1mm1.Adulttick(I.Ricinus)3mmApplicationofareflectorisdifficultonsmallanimalsandstronglyinterfereswithsubsequentbeh
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