微流體芯片課件

MultipleandHigh-ThroughputDropletReactionsviaCombinationofMicrosamplingTechniqueandMicrofluidicChipMembers:李娜馮雙霞徐秋燕劉婷婷聶懷軍themegalleryMultipleandHigh-ThroughputD1ContentsBackgroundExperimentABSTRACTDiscussionContentsBackgroundExperimentA2ABSTRACTMicrodropletso?eruniquecompartmentsforaccommodatingalargenumberofchemicalandbiologicalreactionsintinyvolumewithprecisecontrol.Amajorconcernindroplet-basedmicro?uidicsisthedi?cultytoaddressdropletsindividuallyandachievehighthroughputatthesametime.ABSTRACTMicrodropletso?eruni3Here,wehavecombinedanimprovedcartridgesamplingtechniquewithamicro?uidicchiptoperformdropletscreeningsandaggressivereactionwithreagentconsumption.Ourcombineddeviceprovidesasimplemodeltoutilizemultipledropletsforvariousreactionswithlowreagentconsumptionandhighthroughput.Here,wehavecombinedanimpr4微流體芯片課件5BackgroundAlargenumberofreactionscanbeindependentlyminiaturizedintotinydropletsrangingfromnanolitertopicolitersizeswithouthavingtoincreasethechipsizeandcomplexity.

Microfluidicchipshaveprovidedapowerfulplatformforaccomplishingdropletformationandmanipulationwithinonechipofonlyafewsquarecentimetersinsize.

AdvantagesOverthepastfewyears,droplet-basedmicro?uidicshasattractedincreasingattention.BackgroundAlargenumberofre6Background

Dropletgenerationformultiplesamplesislimitedbythe“world-to-chip”interfaceproblemandcannotbeaddressedindividuallyDropletgenerationandmanipulationfunctionswithinonechipcaneasilyinterferewitheachotherandaredifficultTobalance,despitebeingseparatedbyalong,windingchannel.DrawbackPassivedropletformationmethods:BackgroundDropletgeneratio7InnovateThiscontextshowsahybriddevice:thecartridgedropletgenerationtechniquecombinedwithamicrofluidicchip.Theyimprovedthecartridgetechniquewithanovelmovementscheme:thecapillary,insteadofthemultiwellplate,wasmovedbyadigitalandautomaticmanipulatortoaspiratedifferentsamplesorthecoveringoil.InnovateThiscontextshowsah8Thehybriddropletdeviceforgenerationandmanipulationconsistsoffourmajorcomponents.

(a)manipulator(b)multiwellplate(c)capillary(d)PDMSchipThehybriddropletdevicefor9微流體芯片課件10微流體芯片課件11Threegeneralsteps

loadthemultiwellplatewithdifferentsamples,coverwithalayerofoil,andinputtheparametersofthedesireddropletarrayintothecontrollerviaaPC

startthecontrollertoaspiratecertainvolumesofsamplesandoilintotheCapillarysequentially

introducethegenerateddropletsintothePDMSchipformerging,mixing,detection,orothermanipulation.Threegeneralstepsloadthem12Laboratoryreagent

siliconeoil,

FeCl2·4H2O(Sigma?Aldrich),FeCl3·6H2O(AcrosOrganics),ammoniumhydroxidesolution(28%NH3Fluka),andhydrochloricacid(37%HCl,AcrosOrganics)Laboratoryreagentsiliconeo13ImprovedSamplingTechnique

CombinedDropletDevicePerformance

High-ThroughputDropletScreening

AggressiveDropletReaction

ImprovedSamplingTechniqueCo14ImprovedSamplingTechniqueThecapillaryaspiratestheoilwhenthecantileverremainsinthe“up”state.Whenthecantileveristriggeredtostayinthe“down”state,thecapillaryaspiratesthesample.Theresidencetimeintheoil(TO)andsample(TS)couldbeadjustedrespectivelytogeneratedropletswithdesireddistanceandvolume.ImprovedSamplingTechniqueThe15微流體芯片課件16(c)Relativeintensitywasmeasuredalongthechannelstartingfromtoprightcorner.TheintensityoffluoresceinandDIwaterdropletdoesnotdecreaseorincrease,whichindicatesthatnocross-contaminationbetweenthesetwosampleswasdetected.(b)Dropletarraywasgeneratedbysequentiallyaspirating50uMfluoresceinandDIwater.(c)Relativeintensitywas17CombinedDropletDevicePerformanceHere,themergingofdropletswasdemonstratedinaPDMSchip.ThedropletsfusiondoesnotdependondropletdistancebutdropletvelocityandVd/Vc.Aftermixingcompletely,themergeddropletwasoutputtoasinuouschannelfordisplayanddetection.CombinedDropletDevicePerfor18微流體芯片課件19Inthe?rstapplication,aseriesofAiandBm,n(i=1?2,m=1?3,andn=1?6)weregenerated.A1andA2areFluo-4andRhod-2solutionswiththesameconcentration(10.8μM),whichareindicatorsthatexhibitanincreasein?uorescence(green/red)uponmetalionbonding.Avalueofm=1?3representmetalsionsolutionsofLaIII,YbIII,andYIII,respectively,whilen=1?6aresixdi?erentmetalionconcentrations(0,2.5,5,10,20,and40μM)ofthesesolutions.Thedropletarraycontains721-nLdropletsInthe?rstapplication,aser20High-ThroughputDropletScreeningHigh-ThroughputDropletScreen21微流體芯片課件22The36pairsofdropletreactionsused～72nLofsolutionandrequired1.8mintocomplete.Therefore,thereagentconsumptionforeachmeasurementcouldbereducedto1nLpersample,andthereactionthroughputis1200h?1,whichcanbemuchfasterbyincreasingthe?owvelocity.ThescreeningdropletscouldbestoredinthePDMSchipafterwestoppedthevacuum.The36pairsofdropletreacti23AggressiveDropletReactionAggressiveDropletReaction24Fromtheseimages,wefoundthatthecoprecipitationissofastthatitimmediatelyformsparticleswhentwodropletscomeintocontactwitheachother.Moreover,ineachvolumeratio,theprecipitationinitiatedbythefusionoftwodropletsdidnotblockthemicrochannelatsuchhighconcentrations.Such?exible,controllableandminiaturizedreactionconditionsareextremelysuitableforaggressive,fast,orevenexplosivereactionsthatgenerateprecipitationorheat.Fromtheseimages,wefoundt25DiscussionThecartridgedropletgenerationmethodprovidesanefficientwayforintroducingdifferentnanoliterdropletsintothemicrofluidicchip.themicrofluidicchipoffersaplatformfordelicatemanipulationsuchasmerging.Thisfeatureisvaluableforreducingthereactionscreeningandoptimizationcostandtimeandisparticularlyusefulfordrugscreening.DiscussionThecartridgedrople26Comparedwiththeothercartridgedropletgeneration,ourdesignusedadigitalandautomaticmanipulatortomovethecapillaryinsteadofthemultiwellplate.DiscussionSubsequently,thecoveringoilwasingeniouslyusedasacarrier?uid(continuous-phase)toavoidevaporationandcross-contamination.Comp