GB/T 44738-2024飼用果膠酶活力的測定

ICS

65.120

CCS

B46

中華人民共和國國家標準

GB/T44738—2024

飼用果膠酶活力的測定

Determinationoffeedpectinaseactivity

2024-10-26發(fā)布2025-05-01實施

國家市場監(jiān)督管理總局發(fā)布

國家標準化管理委員會

GB/T44738—2024

目次

前言

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Ⅲ

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范圍

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規(guī)范性引用文件

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術語和定義

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原理

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試劑或材料

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儀器設備

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樣品

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試驗步驟

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8.1

試樣溶液的制備

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8.2

標準曲線繪制

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8.3

測定

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試驗數據處理

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精密度

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Ⅰ

GB/T44738—2024

前言

本文件按照GB/T1.1—2020《標準化工作導則第1部分：標準化文件的結構和起草規(guī)則》的規(guī)

定起草。

請注意本文件的某些內容可能涉及專利。本文件的發(fā)布機構不承擔識別專利的責任。

本文件由全國飼料工業(yè)標準化技術委員會（SAC/TC76）提出并歸口。

本文件起草單位：武漢新華揚生物股份有限公司、北京昕大洋科技發(fā)展有限公司、廣東溢多利生物

科技股份有限公司、山東隆科特酶制劑有限公司、諾維信（中國）投資有限公司。

本文件主要起草人：周櫻、徐麗、詹志春、趙麗芳、馮國華、張法玲、孫薛蔚、蘇丹、余艷、李陽源、

王克芬、王金鳳。

Ⅲ

GB/T44738—2024

飼用果膠酶活力的測定

1范圍

本文件描述了飼用果膠酶活力的分光光度測定方法。

本文件適用于飼料添加劑果膠酶和含有果膠酶的混合型飼料添加劑酶制劑中果膠酶活力的測定。

本文件不適用于堿性果膠酶活力的測定。

本方法定量限為10U/g（或U/mL）。

2規(guī)范性引用文件

下列文件中的內容通過文中的規(guī)范性引用而構成本文件必不可少的條款。其中，注日期的引用文

件，僅該日期對應的版本適用于本文件；不注日期的引用文件，其最新版本（包括所有的修改單）適用

于本文件。

GB/T6682分析實驗室用水規(guī)格和試驗方法

GB/T20195動物飼料試樣的制備

3術語和定義

下列術語和定義適用于本文件。

3.1

果膠酶活力單位pectinaseactivityunit

在37℃、pH3.5的條件下，每小時從質量濃度為2.25mg/mL的果膠溶液中分解釋放1mg還原糖所

需要的酶量。

注：以U表示。

4原理

果膠酶水解果膠溶液產生還原糖，用3，5?二硝基水楊酸顯色，以分光光度計比色測定，計算果膠

酶活力。

5試劑或材料

除非另有說明，僅使用分析純試劑。

5.1水：GB/T6682，三級。

5.2氫氧化鈉溶液（200g/L）：稱取氫氧化鈉20.0g，加水溶解，冷卻至室溫后定容至100mL。

5.3檸檬酸溶液（0.1mol/L）：稱取一水檸檬酸21.01g，加水溶解并定容至1000mL。

5.4檸檬酸三鈉溶液（0.1mol/L）：稱取二水合檸檬酸三鈉29.41g，加水溶解并定容至1000mL。

5.5檸檬酸?檸檬酸鈉緩沖液（0.1mol/L，pH=3.5）：取檸檬酸溶液（5.3）700mL，檸檬酸三鈉溶

液（5.4）300mL，混勻，用檸檬酸溶液（5.3）或檸檬酸三鈉溶液（5.4）