國內(nèi)外水果真菌毒素的限量及檢測方法標準分析匯總

1、720162 7 no. 2feb , 2016joiunal of food safety and quality王蒙，姜楠，戴瑩，馮曉元.(,().100097)摘要：關鍵詞：；maximum residue levels and testing standards of mycotoxins in fruits inchina and abroadwang meng, jiang nan, dai ying, feng xiao-yuan*(beijing research center for agncultural standards and testing. risk assess

2、ment laboratoiy for agro-products ofthe ministiy of agriculture (beijing). beijing 100097. china)abstract: fruits are especially susceptible to be infested by fungi during harvest, transport and storage, and in consequence, several classes of mycotoxins can simultaneously contaminate fhiits. mycotox

3、ins contamination of fruit products is linked to the use of improper technological processing steps. therefore, many countries have established regulations on maximiuii residue levels for mycotoxins in fhiits and fruit products. this review summarized standards of the maximum residue levels and dete

4、rmination methods for fruit mycotoxins, compared and discussed the existing main mycotoxin standards in commodities, and analyzed the importance and urgency of establishing the maximum residue levels and determination methods for fruit mycotoxins in china. it will provide useful references for analy

5、sts, and also for government to establish national standards. key words: fhiits; mycotoxins; standards; maximum residue levels; detection method(ahemana toxin), (ochratoxin. ot)(patulin. pat), (aflatoxin. aft)u1基金項目：(6154023)(qnjj201518)fund: supported by the beijing natural science foundation (6154

6、023) and beijing academy of agriculture and forestry sciences youthfund (qnjj201518).通訊作者： ，e-mail corresponding author: feng xiao-yuan. professor. beying research center for agricultural standards and testing, room 703. block a. beijingagricultural building, no.11 middle road

7、 of shugxiang garden. haidian district. beijing 100097, china. e-mail 71994-2016 china academic journal electronic publishing house. all rights reserved, 246997spp)9(alteniana920 2761-201120114, gb (aoac)(gb),isoaoac(iso) (cen)， ，助:cenhj.9p-h)9aoac cen9iso2水果

8、中真菌毒素的毒性及限量2.1鏈格抱霉毒素(alteniana spp.)( )，(altertoxm-i altertoxin-ii altenoxin-iii); 4 , aal , ta tb tc tdte.,；5 (tentoxin. ten)ame aoh,【叫 2 ame(h)aohdna fi , dna b15j6,aoh,aohdnab,aoh阿aoh,116171 tea 【峋tea(ca/ mg)60si yekeler 的25 mg/kg tea, 10,tea 99sauer 叫aoh ame alt,29 mg/kg tea atx- i ,tea29 mg/kg

9、tea, tea ame aoh(12,22toumas 網(wǎng)ame aoh ,(,borryiis entered)71994-2016 china academic journal electronic publishing house. all rights reserved. (tenuazomc acid. tea)(wo-tea); 35問1-a-,(alternariol,aoh),(altemanol monomethyl ether. ame)(altenuene, alt); 2ame aoh,tmame aoh scott 125124a

10、me aoh,1920ame / aoh teapat95 悟 kg guo 聞 2006-2010100%zhao 網(wǎng)pat1941 pat8pat50 陽kg.,31 90.3%tea ame45.2%.teaaoh1.8198797.7%,mgkg noser(2778 pg kg100%teapatpat112pat509ngkg,cacpat50pg/kg。，gb(efsa)2761-2011850(ttc)咚kgpat(34)12aoh ame995%25 jig/kg2.5 ngkgttc ,aoh,patametea10 pgkg.ttc 1500 ng/kg bw/cfl t

11、ea，2.3赭曲霉毒素2.2展青霉素sipadaro1.6 55.483(aspergillus ochraceus)， ，a b(a carbonarius)cad 4 (ota)ota9網(wǎng) 2004(iarc),2botaota,otaponsone36otapenicilhum ven ucosuni)penicihium expansum l ),(pat) pat網(wǎng) ,patpat,叫ns,pat34.8%.pg/kg 叫 2005-2007pat,6 pat ,7.2%, ota 0 3*20.3 ng/ml.brera 劭ota,ota(7.5 ng/nil)ota表1國內(nèi)外水果

12、及其制品中pat的現(xiàn)行限量標準table 1 standards for maximum pat limits in fruits and fruit products in china and abroad(mg kg)cac codex stan 193-2010(ec) nol 881/2006cpg sec. 510.15050505025105050gb 2761-2011quintela1995-2008ota0 035 ng.ml,2009,ota0 077 ng/ml138 391zhang 40j19992009119otaota2.2 ng/ml,ota網(wǎng)9orv網(wǎng)ota1

13、ota2 pg，kg.ota,2mg kg 10 ng/kg( 2)otaota42gb 2761-2011 ,ota5 ug/kg.ota9384ota,otaota100%,2.4黃曲霉毒素(af),(aspergillus flayus(aspeigtlhisparasiticus),afb afb2afgi afg： afmi afm：,afmi afm：1993,afbi i arc9af,afafaf3,表2國內(nèi)外水果及其制品中ota的現(xiàn)行限量標準table 2 standards for maximum ota limits in fruits and fruit product

14、s in china and abroad(咯 kg)oiv(ec) no 1881/200622ic23表3國內(nèi)外水果及其制品中af的現(xiàn)行限員標準tabic 3 standards for maximum af limits in fruits and fruit products in china and abroad(pg kg)cac-15codex stan 193-2010-10(ec) no 1881/2006 p15(ec) no 165/2010815(ec) no 1058/201281051024610cpg sec. 570(，)-20gb 2761-2011 5-ia

15、c-hplc.tlc 1ota網(wǎng)3 ,afb1,4 af cac網(wǎng)”叫 afafbi 叫:25694 af,afb afb? afg afg276%25% 62% 18%:afbi,11.2hg/kg, 2 0%47,af,3水果中真菌毒素的檢測方法標準研究pat , cen, cen ,aoac6pat5?-593,hplc 2 ,iac-hplc1 , iac-hplcaf 3】tlc 1, iac-hplc 1,(multifiinctional column, mfc)-hplc 1iso cen . aoactlc) , perfbnnance liquid chromatograp

16、hy, hplc) (gas chromatography-mass spectrometry gc-ms)(thin layer chromatography (high(enzyme linked immunosorbent assay elisa)isopat48,494).pataf網(wǎng) 1993 isootaota,isocenota知patpat 32af6,%】2 ,) hplc1 afota5) ) af49pat5 ,1(gb), 1 (sn)2hplc-ms2 ,1(sn), af71-7212 ,(ny)tlc 1(gb) 1iac-hplc13(gb). hplcota.

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28、or-making sci technol, 2012, 1: 107-109.44 .j.2012j9： 115.119.lra fl. zhao zh. xie j research progress of mycotoxms m cereals (jj. guangdon?agnc sa, 2012,19: 115.119.45 cpg sec. 570.200 brazil nuts-adulteration with aflatoxin s.46 cpg sc 570 500 pistachio mits-afljitoxm achilteruon s.47 , .4j. 2o15t

29、 25(13)：2210-2212.bai cl ma bb. yu q. ef al. surveillance quality assessment of the 4 xfhtoxins in market - sale roasted 3ds and nuts m hubei j. chm j health lab tedmol. 2015,25(13)： 2210.2212.48 iso 8128-1-1993 apple juice, apple juice concentrates and dnnks containing apple juice- dttemunation of

30、patulmcontent parti: mttiiod using high-performance liquid chromatography s.49 isos128-2-1993 apple jxuee.apple juice concentrates and dnnks containing apple juice-determixution of pahilmcontent. part 2: method using thin-layer chromatography s.50 iso 16050 2003 foodstuffs dttermuution of aflatoxin

31、bh and the total content of aflatoxins bi, b：, gi and g in cereals, nuts and denied ftoducts-high-performance liquid chromatopaphic method s.51 en 14177： 2003 foodstufr-determination of pahdin m clwr and cloudy apple j rnce and puree hplc method with liquid liquid partition clean-xip s.52 en 15s901

32、2010 foodstuffs-detenmnation of patulm m fruit juice and fruit based products for infants and young children-hplc method f*ithliquid liqwd partition cleanup and 811d phase extraction and uv detection s.53 en 14133: 2009 foodstuffs-detexmination of ochratoxixu m wine ud beer-hplc method with immunoaf

33、iimty column clean-up s.54 en 15829: 2010 foodsniffs-detenum-ition of ochratoxin a m cuirmts. raisins, sultanas, mixd dned fruit and dned figs-hplc method with cohmm cleanup and fluorescence detection s.55 en 14123: 2007 foodsmffs-detenniiution of aflatoxin bi and the sum of aflatoxinb卜 b:. g|and g:

34、 in hazelnuts, peanxits. pistachios, figs and paprika pwder-hplc method with post-column dtntitisation 2nd immunoaflirntj- column cleanup s.56 en iso 16050： 2011 foodstufifs-detenxunation of aflatoxmb .and the totjj content of aflatoxins bh b2. g( and g： m ctrtals. nuts and derived products- high-pe

35、rformance liquid chromatographic method(lso 16050： 2003) s57 aoac 97418 pandm in apple juice thm-hj-er chromatographic method s.58 aoac 995 10 patulin in apph jmct liquid chronutographic method s59 aoac 2000.02 patulm m clear and cloudy apple juices and apple puree liquid chroraa to graphic method s.60 aoac 2001.01 deten