常用官能團的紫外性質

1、.表3-1一些常用官能團的代表摩爾吸收系數(shù)值化合物類型發(fā)色團波長（nm）摩爾吸光系數(shù)胺nh2195苯c6h6184467002026900255170不飽和共軛（c=c）326035000（c=c）430052000（c=c）5330118000不飽和脂肪族c=c1908000（c=c）221023021000不飽和脂環(huán)族（c=c）223026030008000碘化物i260400疊氮c=n1905000二硫化物ss1945500255400砜so2180復雜化合物c=ccc2916500c=cc=n22023000c=c=c=o21025010002000300350弱c=cno222995

2、00腈cn160聯(lián)苯c12n1024620000硫醇（巰基）sh1951400硫醚so19446002151600硫酮c=s205強醚o1851000偶氮n=n285400325醛類cho2101500280300118羧類cooh2002105070酮類c=o19510002702851530硝基no2210強硝酸鹽o no227012溴化物br280300肟noh1905000亞砜s=o2101500亞硝基n=o302100亞硝酸鹽o no2202301000200030040010乙炔cc1751806000酯coor20550表3-2 反相流動相組分的uv吸收值與波長的關系精品.在規(guī)定

3、波長處（nm）的吸收度（au）200205210215220230240250260280溶劑 乙腈0.050.030.020.010.010.01 甲醇2.061.000.530.370.240.110.050.020.01除氣后1.910.760.350.210.150.060.022.52.52.52.52.52.52.52.52.51.45酸和堿醋酸1%2.612.632.612.432.170.870.140.010.01鹽酸6mm（0.02%）0.110.020.01磷酸，0.1%0.01三氟醋酸0.1%水溶液1.200.780.540.340.200.060.020.010.1%

4、乙腈溶液0.290.330.370.380.370.250.120.040.010.01磷酸銨氫二銨，50mm1.850.670.150.020.01三乙胺，1%2.332.422.502.452.371.960.500.120.040.01緩沖液和鹽醋酸銨，10mm1.880.940.530.290.150.020.01碳酸氫銨，10mm0.410.100.010.01edta（乙二胺四乙酸），二鈉，1 mm0.110.070.060.040.030.030.020.020.020.02hepesn-（2-羥乙基）哌嗪-n-2-乙烷磺酸，10mm ph7.62.452.502.372.081

5、.500.290.030.01mes2-（n-嗎啉代）乙基磺酸 ，10mm ph6.02.422.381.890.900.450.060.01磷酸鉀，二氫鹽，10mm0.030.01氫二鹽，10mm0.530.160.050.010.01精品.醋酸鈉，10mm1.850.960.520.300.150.030.01氯化鈉，1m2.001.670.400.100.01枸櫞酸鈉，10mm2.482.842.312.021.490.540.120.030.020.01甲酸鈉，10mm1.000.730.530.330.200.030.01磷酸鈉，100 mm，ph6.81.990.750.190.0

6、60.020.010.010.010.010.01tris-鹽酸，20 mmph7.01.400.770.280.100.040.01ph8.01.801.901.110.430.130.01表面活性劑brij35（23月桂醚），1%0.060.030.020.020.020.010.01chaps（3-3-膽胺丙基二甲基氨溶）-1-丙烷磺酸鹽，0.1%2.402.321.480.800.400.080.040.020.020.01sds（十二烷基硫酸鈉），0.1%0.020.010.01triton x-100（辛醇），0.1%2.482.502.432.422.372.370.500.250.671.42吐溫20，0.1%0.210.140.110.100.090.060.050.040.040.03表3-3 在220nm時，低背景吸收（25 mm磷酸鉀（或鈉）（p h5）25 mm磷酸鈉（或鉀）（p h1.01.01.01.01.01.00.10乙醚1.01.00.460.271.080.100.05乙烷；0.540.200.070.030.020.010.00二氯乙烷1.01.01.01.40.090.110.05甲基-叔-丁醚1.00.690.540.450.260.110.05正丙醇1