豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇課件

1、豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇1豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇先靈葆雅動(dòng)物保健市場(chǎng)技術(shù)部榮 強(qiáng)豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇2mycoplasma hyopneumoniae豬支原體肺炎(mycoplasma pneumonia of swine mps)enzootic pneumonia (ep)豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇3nfirst isolated from pig lungs in 1965n1965年首次在豬肺臟中分離none of the most common and economically important diseasesn最普遍和經(jīng)濟(jì)意義重大的疾

2、病之一na complex interaction between m. hyo and other bacterial and viral infections, and poor management and ventilation.n主要損失是支原體和其它細(xì)菌病毒的混合感染、飼養(yǎng)管理不善、通風(fēng)不良等綜合因素所致。豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇4none of the primary pathogens causing porcine respiratory disease complex (prdc)n引起prdc 的原發(fā)病原之一naffects as many as 99% of

3、u.s. herdsn99的美國(guó)豬群都有感染nworld-wide significancen世界范圍內(nèi)養(yǎng)豬生產(chǎn)中流行豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇5neconomic effects are extremely variable between herds. n不同豬群的經(jīng)濟(jì)影響差異較大ndependent on cost of inputs, price of pigs, other diseases, etc.n取決于投入的成本、豬價(jià)、其它疾病的情況等。na moving target.豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇6nreduced average daily gain (ad

4、g)n降低日增重nreduced feed efficiency (fe)n降低飼料報(bào)酬nincreased medication costsn增加藥物成本nincreased cull or light-weight pigsn增加淘汰或低體重的豬netc.豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇7豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇8nm. hyo decreased growth rate 12.7% and decreased feed efficiency by 13.8% (pointon, et al,1985)n肺炎支原體可降低生長(zhǎng)率12.7%和飼料報(bào)酬13.8%豬肺炎支原體的免疫時(shí)機(jī)

5、及疫苗的選擇9ntransmission between pigs: aerosol and direct contactn豬之間傳播途徑：空氣和直接接觸ntransmission between farms: movement of infected pigs n豬場(chǎng)之間傳播方式：感染豬的移動(dòng)naerosol between farms: britain 3.2 kmn豬場(chǎng)之間傳播距離：3.2公里豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇10clinical signs include:臨床癥狀nchronic cough (often dry or nonproductive)n慢性咳嗽（通常是干

6、咳）nhigh morbidity and low mortalityn高發(fā)病率和低死亡率nuneven groups of pigsn豬群內(nèi)生長(zhǎng)不整齊豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇11npeak prevalence in finisher because of:n在育肥期多發(fā)的原因：nlong incubation period：潛伏期長(zhǎng)nslow spread傳播緩慢nincreased animal density養(yǎng)殖密度加大npresence of other organisms其他致病菌的出現(xiàn)豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇12nm. hyo colonizes cilia

7、of trachea and bronchi.paralyzing then tangling, splitting and breaking cilia.reducing normal function of mucociliary apparatus.n支原體定植在氣管和支氣管纖毛上，導(dǎo)致纖毛纏繞折斷、脫落，從而降低了粘液纖毛的正常功能。豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇13only attaches to tracheobronchial cilia豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇14豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇15豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇16nthere is als

8、o evidence of impaired alveolar macrophage function (caruso and ross, 1990)n破壞肺泡巨嗜細(xì)胞的功能 豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇17豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇18secondary bacterial infections:繼發(fā)細(xì)菌感染npasteurella multocida多殺性巴氏桿菌nactinobacillus pleuropneumoniae胸膜肺炎放線桿菌nstreptococcus suis鏈球菌nhaemophilus parasuis副嗜血桿菌 generally seen as r

9、espiratory distress or “thumping”豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇19nthere are also interactions with several viruses including swine influenza virus, pseudorabies (prv), prrs，pcviin還和幾種病毒相互作用，其中包括豬流感病毒、偽狂犬病毒、藍(lán)耳病病毒、圓環(huán)病毒ii型豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇20豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇21豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇22mycoplasma hyopneumonia: control and

10、prevention豬支原體肺炎的控制與防治豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇23nmedication藥物nmanagement管理nvaccination免疫豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇24nantibiotics have shown mixed results in field conditions and are used mostly to control secondary infections.n田間條件下，抗生素的效果表現(xiàn)不一，主要用于控制繼發(fā)感染。豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇25nlincomycin: results inconsistentn林可霉素ntia

11、mulin: results inconsistentn支原凈nquinolones: show some promisen喹喏酮類nflorfenicol: shows some promisen紐弗羅naivlosin: results are goodn愛(ài)樂(lè)新豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇26none problem with any medication program is that once the medication is removedthe disease can begin to cause problems again.n一旦停藥，疾病復(fù)發(fā)豬肺炎支原體的免疫時(shí)機(jī)及疫

12、苗的選擇27management factors are important管理十分重要nall-in/all-out production is probably the single most important management techniquen全進(jìn)全出是最好的管理方式nno more than 3 weeks spread in agen年齡差距不要超過(guò)3周nmedicated early weaning (mew) has been shown to help control the mps diseasen早期藥物斷奶有一定幫助豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇28mana

13、gement factors (continued)nsegregated early weaning (sew) has shown meritn早期隔離斷奶也有一定好處nvaccination免疫noptimize density and proper ventilationn合理化密度，保持良好通風(fēng)豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇29key to an effective program to control prdc (porcine respiratory disease complex) is an effective m. hyo vaccine控制prdc有效措施的關(guān)鍵是使用

14、有效的支原體疫苗豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇30nbecause the exact antigenic epitopes responsible for immunity are not well understood, whole-cell vaccines are best.n因?yàn)檫€不清楚決定免疫力的具體抗原決定部位，所以全細(xì)胞疫苗是最好的。豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇31nm+pac preserves 15 proteinsn安百克保留了15種抗原蛋白nproduct a preserves 11 proteinsna產(chǎn)品保留了11種抗原蛋白nproducts b and

15、 c preserve 8 proteinsnb和c產(chǎn)品保留了8種抗原蛋白豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇32豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇33nalso, because long-lasting immunity is important, it is our belief that an effective m. hyo vaccine should have an improved oil adjuvant.n此外，長(zhǎng)久免疫力是重要的，良好的油佐劑是支原體疫苗必須的。豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇34vaccine adjuvants疫苗佐劑豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇

16、35naluminum hydroxide (ah)氫氧化鋁nwater-in-oil (w/o)油包水noil-in-water (o/w)水包油豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇36nlow viscosity粘度低nmild to no reactions溫和無(wú)刺激nimmediate response免疫反應(yīng)迅速nshort-lived immunity免疫期短豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇37nmore oil = more viscosity粘度高nmore irritation刺激大nmore pain疼痛nmore stress應(yīng)激大豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇38n

17、more modern最新型nat least as effective as w/o與油包水效果相同nless oil = lower viscosity低粘度nless irritation刺激小nless pain疼痛少nless stress (pigs and people)應(yīng)激小豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇39nm+pac spah o/w+ahnrespisure pfizer o/w,lecithin卵磷脂nrespifend fort dodge ahningelvac mh nobl/bi w/onporcilis intervet ahnhyoresp merial

18、 ah豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇40introducing new m+pac豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇41nreduced lung lesionsnless coughingnhigher antibody titersnhigher cell-mediated immunitynexclusive emunade adjuvantn1 ml administration豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇42nthe key to m+pacs superior performancen安百克優(yōu)良性能的關(guān)鍵nfaster acting, longer lastingn速效、

19、長(zhǎng)效nexcellent syringeabilityn良好的通針性豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇43naluminum hydroxide plus oil-in watern氫氧化鋁水包油nfast acting速效nlong lasting長(zhǎng)效nstimulates strong cell-mediated immunityn刺激堅(jiān)強(qiáng)的細(xì)胞介導(dǎo)免疫力豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇44oil globules in emunade contain antigen both inside droplet and on droplet surface. surface antigen

20、prompts rapid immune response.豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇45豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇46antigen inside is released gradually as oil droplets break down over time. this gradual release allows emunade to deliver prolonged immunity.豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇47豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇48ntiming (later is better?)時(shí)機(jī)none-dose vs. two-dose一針還

21、是二針nsow vaccination母豬免疫豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇49nlater is better.why?n越晚越好nmaternal antibody interferencen母源抗體干擾nprotection needs to last for entire finishing periodn保護(hù)力需要持續(xù)整個(gè)育肥期豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇505.95.818.8005101520% of lung lesions4 weeks6 weeksnonvaccnon-chall* significantly lower than nonvaccinated, p

22、 3rd parity sowsn經(jīng)產(chǎn)3次的母豬抗體滴度下降。ndecreased sow to pig shedding?n能減少母豬對(duì)仔豬的散毒嗎？豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇56nif sow herd is vaccinated, especially pre-farrowing, then it is even more important to vaccinate pigs later.n如果母豬免疫，尤其在產(chǎn)前免疫，仔豬則免疫更嘉推遲。豬肺炎支原體的免疫時(shí)機(jī)及疫苗的選擇57convenient dosage schedule方便的劑量n1 ml dose at 7-to-10 days of age or ol