AKTAavanton詳細(xì)介紹.

1、?KTAavant? 25/150 On -Site TrainingGE Imag in ation At Work2/?KTAava nt? and UNICORN? 6.0I nstrume nt front正面1 Fraction collector 收集器2 Buffer tray緩沖液托盤3 In strume nt display 顯示屏4 Wet side 側(cè)面5 Foldable door 折疊門6 Pump cover泵的保護(hù)門Holder rails樣品支架7 Swivel foot Lock/Unlock knob 底座鎖 9 Swivel foot 旋轉(zhuǎn)底座 10 S

2、wing out toolbox工具盒11 Power switch電源開關(guān)Locatio n of comp onents3/?KTAava nt? and UNICORN? 6.01 nstrume nt display顯示屏方便的顯示系統(tǒng)相關(guān)參數(shù)Function?顯示設(shè)備的狀態(tài)，運(yùn)行數(shù)據(jù)和系統(tǒng)參數(shù)?顯示收集器是否工作在顯示屏上方便實(shí)現(xiàn)暫停（Pause和繼續(xù)（Continue Details?觸摸屏Other comp onents4/?KTAava nt? and UNICORN? 6.01 nstrume nt wet side1 Injecti on valve 樣品閥2 Flow

3、restrictor 限流器3 pH valve pH 計(jì)閥4 Colu mn valve 柱位閥5 UV monitor UV 檢測(cè)器6 Conductivity monitor 電導(dǎo)檢測(cè)器7 In let valve B入口 B8 Quaternary valve四通閥 9 System pump B系統(tǒng)泵 B10 Pressure mon itor of System pumps系統(tǒng)泵的壓力傳感器11 In let valve A入口 A12 System pump A系統(tǒng)泵 A 13 Mixer 混合器14 Pressure mon itor of Sample pump樣品泵的壓力傳

4、感器15 Sample pump樣品泵 16 Pump pist on rinsing system tubes泵后潤洗管17 Sample in let valve 樣品入口 18 Outlet valve 出口閥19 Holder rails柱架5/入口 B?KTAava nt? and UNICORN? 6.0入口 Aimagination at workimagination at work6/?KTAava nt? and UNICORN? 6.01 nlet valves A and B 入口閥自動(dòng)的在不同的buffer和清洗液之間變換Function?在緩沖液和清洗液之間變換？將

5、緩沖液A和B混合產(chǎn)生梯度？整合了空氣感應(yīng)器Details?7個(gè)緩沖液入口（A1-A7, B1-B7?1個(gè)連接四通閥的緩沖液入口（Q ?入口的變化 Transposed position of the inietsValvesirriaginalian m： wwi四通閥?KTAava nt? and UNICORN? 6.0Quaternary valve7/imagination at work/?KTAava nt? and UNICORN? 6.0Quaternary valve 四通閥利用四種母液實(shí)現(xiàn)緩沖液的自動(dòng)配制注意：四通閥不推薦使用有機(jī)溶劑Fun cti on?四元梯度？Buff

6、erProDetails? 4個(gè)緩沖液入口 (Q1-Q4?2個(gè)緩沖液出口 (A, BValvesimagination at work9/?KTAava nt? and UNICORN? 6.0BufferProBuffer stock Titra nt stock Water 4 M NaCI9Buffer concen tration can be set 9可以設(shè)置緩沖液的濃度9Actual mixing ratio for all stock solutionsMixi ng ratio both as curves and Run Data 9緩沖液的混合比例真實(shí)體現(xiàn)imagmatt

7、on at work10/BufferPro時(shí)的其它硬件? KTAava nt? and UNICORN? 6.0 使用四元梯度以及 pH mon itor pH 檢測(cè)器Quater nary valve 四元閥Mixer混合器新的設(shè)計(jì)4個(gè)非?？焖僮儞Q（7秒的電磁閥.不能使用乙腈在低離子強(qiáng)度下提高PH電極更加精確PH探頭的標(biāo)定和保存更加方便EasyNote: pH計(jì)只檢測(cè)不反饋Easy改變混合器的體積11/?KTAava nt? and UNICORN? 6.0A PumpB Pump12imagination at work/?KTAava nt? and UNICORN? 6.0Syste

8、m pumps 系統(tǒng)泵系統(tǒng)泵在純化過程中可以產(chǎn)生等度或者梯度洗脫功能?等度洗脫？梯度洗脫Details?系統(tǒng)泵A and B?流速范圍 0.001 -25 ml/min0.01150ml/min?青洗閥 ?泵活塞的潤洗系統(tǒng)PumpsPump pist on rinsing system 泵后潤洗1樣品泵2系統(tǒng)泵A3系統(tǒng)B每個(gè)50ml潤洗系統(tǒng)管中充滿了20%乙醇Wash OUTWashINBufferINBufferOUTBall outletcheckvalveBall in let checkvalve活塞泵后清洗隔離 膜IN-LINE單向閥耐高壓的活塞密封圈13/?KTAava nt? a

9、nd UNICORN? 6.014/?KTAava nt? and UNICORN? 6.0手動(dòng)潤泵以及排氣更換緩沖液以及排氣需要在軟件中采取泵洗操作(pumpwashBuffer INAir?KTAava nt? and UNICORN? 6.0System pump pressure泵的壓力傳感器AKTAava nt 25:020MPa AKTAava nt150:05Mpa帯imagination at work?在線濾器（pore size 10三種m不同大小的混合器?在線濾器（pore size 10三種m不同大小的混合器16/?KTAava nt? and UNICORN? 6.0

10、Mixer 混合器?在線濾器（pore size 10三種m不同大小的混合器?在線濾器（pore size 10三種m不同大小的混合器17/?KTAava nt? and UNICORN? 6.0Mixer混合器實(shí)現(xiàn)緩沖液成份的均勻混合Fun cti on?混合從系統(tǒng)泵流出緩沖液Details?在線濾器（pore size 10三種m不同大小的混合器Other comp onents Mixerchamber size Flow ratetwo pump gradie nt Flow ratequater nary gradie nt0.6 ml < 2 ml/min < 1 ml

11、/min1.4 ml 2 -2 ml/min 1 ml/min 5 ml 12 -25 ml/min 6 -25 ml/min In-line filter 在 線濾器18/?KTAava nt? and UNICORN? 6.0Mixer混合器不同流速下需要使用不同大小的混合器 0-1 ml/min 0.6 ml mixer 1-6 ml/min 1.4 ml mixer 6-25 ml/min 5.0 ml mixerQ1,2,3,4 mix With BufferPro or Q-gradimagi notion ot work1.Q-閥在很小的體積里從Q1到Q4間切換流速越大，混合器體

12、積越大2. A,B兩個(gè)泵同步化為一個(gè)泵產(chǎn)生穩(wěn)定的流速3. 混合器必須有一定的體積去正確的梯度和緩沖液Cond Cond0.6 ml mixer at 20 ml/min19/?KTAava nt? and UNICORN? 6.0lnject valve上樣閥imagination at work20/亠亠二亠丄鼻亠亠Jl亠Jimagination at work?KTAava nt? and UNICORN? 6.0lnjectio n valve上樣閥實(shí)現(xiàn)樣品上樣Function?樣品可以使用以下方法上樣：?用注射器或者樣品泵充滿樣品環(huán)（Loop?用注射器或者樣品泵充滿SuperLoop

13、?羊品泵直接上樣?上樣閥的設(shè)計(jì)使得使用不同上樣方式時(shí)，不用改變管路連接?Details?所有泵的流路 ?羊品環(huán)/超級(jí)上樣環(huán)Sample loop/Superloop注射器的連接 Valves21/ ?KTAava nt? and UNICORN? 6.0Injection valve上樣閥在上樣閥的LoopF(fill和LoopE (empty位置連接樣品環(huán)(或者超級(jí)上樣環(huán)?KTAava nt? and UNICORN? 6.0lnjectio n valve上樣閥原理22/23/?KTAava nt? and UNICORN? 6.0 Inject valve柱位閥24/?KTAava nt

14、? and UNICORN? 6.0Colu mn valve柱位閥實(shí)現(xiàn)不同柱位的選擇Function?I ntegrated pressure sen so整 合壓力傳感器空柱子壓力Column hardware -填料反壓Column media ?整合了旁路(bypass (systemwash系統(tǒng)清洗？整合了流向閥 Details?最多連接5根柱子?整合了柱子壓力傳感器-nlet port進(jìn)口-Outlet port 出口Valves25/?KTAava nt? and UNICORN? 6.0Colu mn valve柱位閥26/imagination at work26/?KTAa

15、va nt? and UNICORN? 6.0 柱子壓力傳感檢測(cè) 實(shí)現(xiàn)柱子前后的壓力檢測(cè)DetailsPre colu mn pressure mon itor (inlet of the colum n valvdi 前壓力Post colu mn pressure mon itor (outlet of the colu mn valvit 后壓力27/?KTAava nt? and UNICORN? 6.0柱子傳感檢測(cè)器實(shí)現(xiàn)柱子前后的壓力檢測(cè)Fun ction ?監(jiān)測(cè)加在柱子硬件上的壓力-pre column pressure26/?監(jiān)測(cè)加在填料上的壓力-delta column pre

16、ssure調(diào)節(jié)流速防止超壓恒壓裝柱Colum n bottompressure 0.1 MPaColum n top pressure 0.4 MPaSystem pumppressure 0.6 MPaColum n media pressure (DeltaC P0.3 MPaColum n hardware pressure 0.4 MPaPreC PPostC P?KTAava nt? and UNICORN? 6.0UV監(jiān)測(cè)器電導(dǎo)監(jiān)測(cè)器28/29/檢測(cè)器?KTAava nt? and UNICORN? 6.0 UV mon itor UV實(shí)現(xiàn)連續(xù)的UV吸光度檢測(cè)Function?檢測(cè)

17、緩沖液和洗脫蛋白質(zhì)的UV吸光度值?3個(gè)波長Details?190-700 nm?流通池-2 mm 光徑 default-0 mm光徑?每一個(gè)流通池真實(shí)的光徑Mon itors30imoginoti onot work/?KTAava nt? and UNICORN? 6.OC0 nductivitymon itor電導(dǎo)檢測(cè)器實(shí)現(xiàn)電導(dǎo)（緩沖液鹽濃度的連續(xù)監(jiān)測(cè)Function?檢測(cè)緩沖液和洗脫蛋白質(zhì)的電導(dǎo)變化Details?電導(dǎo)常數(shù)是工廠校準(zhǔn)的（The cell constant is factory-calibrated內(nèi)置溫度檢測(cè)Mon itorsTemperature monitor實(shí)現(xiàn)連續(xù)

18、的溫度監(jiān)測(cè) Fu nction ? ? 1最小化溫度對(duì)電導(dǎo)的影 補(bǔ)償 pH 值（Compensates the pH value BufferPrc中使用 Monitors 溫度檢測(cè)器 Details 內(nèi)置溫度檢測(cè)器 31 / ?KTAavant? and UNICORN? 6.0 Train the Trainer -In strume nt Details / 10/06/2009pH 閥 32 / ?KTAavant? and UNICORN? 6.0 Train the TrainerInstrumentDetails / 10/06/2009pH monitor實(shí)現(xiàn)pH的連續(xù)監(jiān)測(cè)Fu

19、nction ?檢測(cè)緩沖液和洗脫蛋白質(zhì)的 pH Monitors Details ? ?整合pH閥改進(jìn)pH探頭-對(duì)低離子強(qiáng)度更加敏感 更寬的pH 線性范圍 33 / ?KTAavant? and UNICORN? 6.0 Train the TrainerInstrumentDetails / 10/06/2009pH valve pH閥在測(cè)量pH的同時(shí)也整合了一個(gè)反壓閥。Valves功能？ ？ ？使用者使用的時(shí)候可以選擇使用一個(gè)或 者全部的功能，也可以選擇不使用。整合的反壓閥能防止氣泡在UV流通池的 生成pH電極能在設(shè)備的旁路中保存以及標(biāo)定。 Details ? ? ?整合了 pH流通池pH

20、電極 標(biāo)定 限流器（0.2 MPa 34 / ?KTAavant? and UNICORN? 6.0 Train the TrainerIn str-me nt Details / 10/06/2009pH valve 35 / ?KTAava nt? and UNICORN? 6.0 Train the Trai ner In strumentDetails / 10/06/200936/?KTAava nt? and UNICORN? 6.0 出口閥37/37/37/37/imaginotion at work37/imagination at work?KTAava nt? and UN

21、ICORN? 6.0Outlet valve出口閥大體積樣品的收集Function?出口閥收集?收集器收集Details?10個(gè)出口 (Out 1-Out10?廢液(Waste?攵集器出口 Valves38innagination at work/?KTAava nt? and UNICORN? 6.0? 內(nèi)置的收集器?溫度調(diào)節(jié)控制?同時(shí)可以放置進(jìn)去6個(gè)收集盤?不同的收集盤有不同的收集體積？收集盤能自動(dòng)的被掃描儀檢測(cè)?KTAava nt? and UNICORN? 6.0 實(shí)現(xiàn)靈活的收集Function舔羊品試管架?儲(chǔ)存時(shí)收集試管架?快速釋放試管Details/Available casset

22、tes8, 15 or 50 ml 試管-深孔板（24, 48, 96 wells?每種cassette使用一種試管類型?每種cassette可以使用三種類型的深孔板？不同cassette代碼代表不同的類型40/?KTAava nt? and UNICORN? 6.0Detection of cassett es 掃描實(shí)現(xiàn)自動(dòng)檢測(cè)不同類型的cassettesFunction?掃描cassette代碼來識(shí)別使用的cassettes的 類型Details?快速掃描-識(shí)別當(dāng)前cassettes的類型?全面掃描-識(shí)別使用了哪種類型的深孔盤（24, 48 or 96 deep wellsFracti o

23、n collector41/?KTAavant? and UNICORN? 6.0Spill -free fractionation AKTAavant 25 有兩 個(gè)特點(diǎn)能避免收集的時(shí)候漏出管子Function?避免收集時(shí)試管和收集孔中溢出 Details :?Drop-sync檢測(cè)每一滴的釋放？Dropsync能在流速2ml/min的使用Accumulator儲(chǔ)存器,在高流速的時(shí)候，當(dāng)一根管子移動(dòng)到下一根管子的時(shí)候能在短時(shí)間內(nèi)保存液滴。Fracti on collector42/?KTAavant? and UNICORN? 6.0Delay volumes 延遲體積 In the Sys

24、temSett ings dialogof System Con trol, selectFracti on collecti on:Delayvolumes to view an d, ifn ecessary, edit the delayvolumes.43/?KTAava nt? and UNICORN? 6.0 樣品泵樣品泵壓力傳感器樣品入口閥44imagination at work/?KTAava nt? and UNICORN? 6.0Sample pump 樣品泵使用樣品泵直接把樣品 加到柱子上或者充滿樣品環(huán)Details?流速范圍 0.01-25 ml/min?最大操作壓力

25、10 MPa?青洗閥?泵活塞的潤洗系統(tǒng)Pumpsimagination at workimagination at work45/?KTAava nt? and UNICORN? 6.0Sample in let valve 樣品閥自動(dòng)的使用不同的樣品上樣Function?選擇不同的樣品入口?lntegrated air sensor for injecting all sample整合空氣感應(yīng)器？專門的緩沖液入口- 羊品上樣前使用緩沖液沖洗樣品泵懷同運(yùn)行之間沖洗樣品入口Details?7個(gè)樣品入口（S1-S7?1個(gè)緩沖液入口上樣前后清洗樣品管路（一般使用平衡緩沖液ValvesOverview 1. Sample in let valve (V9-IS 樣品閥 2. I nlet valve A (V9-IA 緩沖液入口 A 3. Inlet valve B (V9-IB 緩沖液入口 B 4. Quaternary valve (Q9 5. Injection valve (V9- Inj 6. Column valve (V9-C 7. pH valve (V9-pH 8. Outlet valve (V9-O 四通閥 上樣閥 柱 位閥 pH 閥 出口閥 Val