鑭系配合物發(fā)光性能研究

1、 Synthesis and fluorescence properties of Tb(III) com plex with a novel aromatic carboxylic acid ( L) as well as spectroscopic studies on the interaction between Tb(III) complex and bovine serum albumin Jiqing Bao a, Zhenfeng Zhang b, Ruiren Tang b, n, Hongxing Han b, Zhengfa Yang b 2013.8l制備出新的配體2,

2、6-bis(3,5-dicarboxypyrazol-1-ylmethyl) pyridine，并合成配合物 TbL(H2O)4Cl .H2O l對配體和配合物通過元素分析、IR, 1HNMR, EIMS 和 TGDSC進(jìn)行表征l 為了探索TbL(H2O)4CL.H2O潛在的生物的活性值，用熒光猝滅光譜研究了TbL(H2O)4CL.H2O與BSA的紫外可見吸收和同步熒光光譜。通過對其熒光淬滅機(jī)理的分析。結(jié)合位點數(shù)n和結(jié)合常數(shù)Ka分別按雙倍計算取對數(shù)回歸方程得到的熱力學(xué)參數(shù)表明范德瓦爾斯和氫鍵作用是主要的分子間力的相互作用。 abstractintroduction 鑭系發(fā)光物質(zhì)越來越受到化學(xué)和

3、生物學(xué)科的關(guān)注，由于它具有很窄的發(fā)射帶和較大的stokes位移以及較長的發(fā)射壽命。他的配合物有優(yōu)良的吸收系數(shù)和高效的配體（III）離子的能量轉(zhuǎn)移功能。BSA是最豐富的血漿蛋白質(zhì)，在壓力膠體滲透血液中的維護(hù)和結(jié)合與各種配體如脂肪酸，激素和藥物的運輸，起著最重要的生理作用。通過稀土配合物與BSA之間的相互作用研究是必要的，和可以獲得大量的稀土配合物在生物信息的作用，這可能對現(xiàn)代藥物的研究部有著重要的影響。experimentalexperimental配合物的合成：To a stirring solution of KOH (0.90 g, 16 mmol) in 25 mLmethanol, L

4、(1.88 g, 4 mmol) was added. The reaction mixturewas stirred at 50 1C for 24 h. After the solvent was evaporated, theresidue was dissolved in 10 mL water. To the solution was addeddropwise 4 M HCL, the resulting white precipitate was collected by filtration, and purified by recrystallization from wat

5、er, to givecompound L (1.5 g, yield: 92%)result and discussion Composition and physical properties of the complexThermal analysisFT-IR spectra and EIMSLuminescence properties 對配合物熒光性能的研究表明鋱（III）離子可以有效地通過配體敏化Analysis of the interaction of complex with BSA 分析化合物對BSA的熒光猝滅作用 結(jié)果表明，在TbL(H2O)4Cl .H2O 和BSA之

6、間有較強(qiáng)的相互作用和非輻射能量轉(zhuǎn)移。且配合物對BSA的熒光猝滅為靜態(tài)猝滅造成的Analysis of the interaction of complex with BSA 計算結(jié)合常數(shù)和結(jié)合位點 熱力學(xué)參數(shù)和結(jié)合位點 the binding constants Ka were greater than or approach to10-5L/MOL, indicating that the complex quenches the fluorescence emission of BSA with higher binding affinity and experiences a stati

7、c quenching procedure。 The negative values of enthalpy DH and entropy DS suggest that the binding is mainly enthalpy driven and the entropy is unfavorable for it, hydrogen bonds and van der-Waals interactions played major roles in reactionAnalysis of the interaction of complex with BSA 同步熒光光譜 實驗結(jié)果表明，關(guān)于BSATbL(H2O)4CL.H2O熒光的猝滅機(jī)理為靜態(tài)猝滅。在不同溫度下的結(jié)合常數(shù)和結(jié)合位點的數(shù)目不同揭示了配合物和BSA的相互作用只有一個單一的結(jié)合位點