均勻設(shè)計(jì)法研究八角油β-環(huán)糊精包合物的制備工藝

1、均勻設(shè)計(jì)法研究八角油-環(huán)糊精包合物的制備工藝         09-09-10 09:09:00     作者：趙星華，盛家榮    編輯：studa20【摘要】  目的研究-環(huán)糊精對(duì)八角油的包合作用。方法 以揮發(fā)油包合率為指標(biāo)，應(yīng)用3因素6水平的均勻設(shè)計(jì)法，篩選出八角油包合物的最佳制備條件，即溫度、研磨時(shí)間、樣品與-CD的比例。結(jié)果實(shí)驗(yàn)得出最佳條件：當(dāng)溫度為35.0，研磨時(shí)間為4.0 h，八角茴香油與-CD的比例113.0（

2、 mlg）時(shí)，包合率為81.4%；紅外測(cè)定包合作用是否完全。結(jié)論優(yōu)選得到的工藝結(jié)果穩(wěn)定可行。 【關(guān)鍵詞】  八角油； -環(huán)糊精； 包合物制備； 均勻設(shè)計(jì)Abstract:ObjectiveTo study the inclusion complex of Star anise oil with cyclodextrin. Methods3 factors and 6 levels of conditions for the preparation of the inclusion complex were selected by the uniform design. The rat

3、io of Star anise oil in the inclusion complex was used as criterion in these experiments. The inclusion complex was made by pestling method. ResultsThe best conditions of the inclusion complex were that: the ratio of Star anise oil and -Cyclodextrin was 1:13.0(mL/g), and grinding for 4.0 hours at 35

4、.0. The ratio of volatile oil in the inclusion complex was about 81.4%, and the inclusion complex was identified by IR. ConclusionThe optimized process is stable and highly efficient.   Key wordsStar anise oil；-Cyclodextrin；  Inclusion process；Uniform design    Guangxi

5、is a Chinese autonomous province, located in a subtropical zone, south west of China. In particular, it has extensive resources of different plants. Among them, there is a special and commercial product, essential oil, which is widely recognized. Essential oils are the volatile, fragrant substances

6、residing in the flowers, fruits, leaves, and roots of many plants1.Most of them are a complex mixture of chemical constituents，including nitrogen and sulfur-containing molecules, aromatic chemicals, and terpenes    The following oils are the most famous and are found in large quantity

7、 in Guangxi. Eucalyptus oi1 has been employed in ointments as a topical counter-irritant and together with menthol as an inhalation1.Star anise oil is the main product in the fruits of Illicium verum. The plant contains remarkable essential oil, which is used as flavoring and medicine to control cau

8、ght2.The related content of trans-Cinnamaldehyde in Guangxi cinnamon oil was 11.58%, higher than that in Yunnan cinnamon oil. These two provinces are the main producing areas of cinnamon oil in China 3.    Star anise oil is separated from the fruits of Illicium verum Hook.f. This plan

9、t is used as flavorings as well as Chinese herbal medicine. In Star anise oil, anethole(structure below) is the major component which has structures as the Cis & trans isomers occurring in nature. Remarkably, the Trans isomer is always being more abundant. Star anise oil has a distinct effect on

10、 raising leucocytes (the white blood-cells), so in American patent, it has been applied in the remedy for anti-leukemia. And it also has been used as poultry feed additive composition4. However, the effectiveness decreases with long time storage, and both physical and chemical characteristics change

11、 relatively.-CyclodextrinX(structure above) has been of interest in numerous industries including the pharmaceutica1 field. This is due to their particular structures which impart interesting physiochemical properties. Today -CD is known for their ability to molecularly encapsulate a wide variety of

12、 the drugs into their hydrophobic cavity, resulting in the enhancement of water solubility and drug dissolution's rate1. The results of physi cochemical tests showed that after inclusion the solubility and stability of paprika pigment, the binding ability of -cyclodextrin for inclusion complex w

13、ith guest curcumin, was improved obviously57.So it is considered as a good supplementary material for injection8.    Up to date, although the uniform design has been widely used in many synthesis and separation fields9,no reports related with the inclusion complex of Star anise oil an

14、d-cyclodextrin, by three factors and six levels uniform design, has been available. However, by using this practical design, the value of Star anise oil would definitely enhance and would benefit many pharmaceutical and commercial industries.1 Reagents    -Cyclodextrin: was obtained f

15、rom Pharmaceutical Manufacturing Factory.Shanghai;Star anise oil: was abstracted from the fruits of Illicium verum Hook.f. All the other chemicals and reagents used were of the reagent grade obtained commercially.2  Methods2.1  Use Uniform Design for the experimentsAmong many factors that

16、effect the preparation of the complex, the temperature, the ratio of oil and CD, and the time to mix two compounds, are most important10.Hence, U*（63）three factors and six levels of the uniform design was considered. To see details, in table 1.2.2  Researches in the inclusion complex2.3 IR diffraction manifestationSince IR is a very useful