九十四學(xué)年度中醫(yī)三微免實驗期末考

1、九十四學(xué)年度中醫(yī)三微免實驗期末考姓名： 學(xué)號： 劉世東老師：25%填充題 (6 %)1. Urease test is to test the ability of microorganism to split urea. The positive result should show _ color. Which of the following microorganism is usually urease positive? _ (choose one: E. coli, Klebsiella pneumoniae, Pseudomonas aeruginasa)2. Nitrate r

2、eduction test is used to examine if the bacterium produces _ (what enzyme?). After adding reagents sulfanilic acid and a-naphthylamine, the positive test should show _ (what color?). To rule out false negative results, you add _ (what reagent?) and the positive result should show _ (what color?)問答題

3、(19 %)1. Gram staining is an important method to distinguish Gram (+) from Gram (-) batreria. Please write down the staining procedure and indicate which the most important step is. (6 %)2. Please give the definition of selective media and differential media. Give an example of each type of media th

4、at used in the experiment and explain purpose. (4 %)3. Explain the purpose of 10% glucose solution, 10% lactose solution and ONPG (ortho-nitrophenyl-b-galactoside) in the enzyme induction experiment. (3%)4. Distinguish between F+ and Hfr bacterial strains. (2 %)5. Please describe the mechanism of op

5、eration of the lactose operon. (4 %)謝絹珠老師：15%1. Mycobacterium tuberculosis causes highly infectious disease in population. The bacterium however can not be stained well with Gram stain, and you use acid-fast stain instead to examine a sputum smear. Why? What is the principle involved in acid-fast st

6、ain? (5 %)2. Fungi are eukaryotes and they are not as small as bacteria. Identification of fungi frequently involved morphology characteristics. To examine a fungus on slide, what stain do you use to reveal the fungal structure? If the fungi are from a patients skin, how do you treat the scraped sam

7、ple in order to reveal your target organism? (5 %)3. The API 20-E system is commonly used to analyze the biochemical profile of enterobacteria. A total of 20 tubes are included. What is the main principle involved in the IMViC test ? (5 %)陳怡原老師：14%1. What is the function of thioglycollate in cultiva

8、tion of anaerobes? (1 pts)2. Describe the expected results when you perform spore staining procedure (Schaeffer-Fulton method) with a Bacillus cereus culture and a Staphylococcus aureus culture. Please include the shape, arrangement and color of each culture. (3 pts)3. Why strict anaerobes can not s

9、urvive in the presence of oxygen? (2 pts) 4. What is the enzymatic activity of oxidase? (1 pt)5. Why can Staphylococcus aureus enhance the growth of Haemophilus influenzae on a blood agar plate? (2 pts)6. What is CAMP test? Which streptococcal sp. can be identified by a positive CAMP test? (3 pts)7.

10、 In addition to the different growth patterns on mannitol salt agar, please name two procedure that are commonly used in the laboratory to distinguish Staphylococcus epidermidis from Staphylococcus aureus. (2 pt)8. What is a-hemolysis? (1 pt)郭敏玲老師：16%1. Please define titer and describe how to determ

11、ine it in a serum sample. (5%)2. What is the main purpose to inject 1ml 4% thioglycollate into the peritoneal cavity of mice for phagocytosis assay? When should the mice be injected? (5%)3. Why is the vaccination against tetanus recommended for everyone and the vaccination against influenza for only

12、 a restricted group of “at-risk” individuals? (3%)4. In a cytotoxic assay against tumor cells, please explain why might the NK cells cause some damage to the tumor and the presence of antibody to the tumor enhance the cytotoxic capacity of the NK cells. (3%) 趙玫老師：30%1. 實驗室診斷結(jié)果是確認病毒感染的重要依據(jù)。請條述常用的六大類診斷方法。(3%)2. 在phage培養(yǎng)實驗中使用maltose的原因為何？何謂plaque? 實驗中如何定量病毒數(shù)量? (6%)3. (1)