USP關(guān)于色譜柱填料的規(guī)定

1、我們在實驗中常常用到USP作為檢驗依據(jù)，其中有一些關(guān)于色譜柱的要求 現(xiàn)將其中個色譜柱要求羅列 如有不恰當(dāng)?shù)?請大家指正L1和L8是美國藥典(USP)規(guī)定的色譜柱編號，其實就是C18柱和NH2柱。下面是對應(yīng)的色譜柱類型。L1：十八烷基鍵合多孔硅膠或無機(jī)氧化物微粒固定相， 簡稱C18或ODSL2：3050um表面多孔薄殼型鍵合C18(ODS)固定相L3：多孔硅膠微粒 即一般的硅膠柱L4：3050um表面多孔薄殼型硅膠L5：3050um表面多孔薄殼型氧化鋁L6：3050um實心微球表面包覆磺化碳氟聚合物強(qiáng)陽離子交換固定相 L7：全多孔硅膠微粒鍵合C8官能團(tuán)固定相 簡稱C8柱L8：全多孔硅膠微粒鍵合非

2、交聯(lián)NH2固定相 簡稱NH2柱L9：強(qiáng)酸性陽離子交換基團(tuán)鍵合全多孔不規(guī)則形硅膠固定相L10：多孔硅膠微球鍵合氰基固定相（CN） 簡稱CN柱L11：鍵合苯基多孔硅膠微球固定相 簡稱苯基柱L12：無孔微球鍵合季胺功能團(tuán)的強(qiáng)陰離子填料L13：三乙基硅烷化學(xué)鍵合全多孔硅膠微球固定相（C1） 簡稱C1柱L14:10um硅膠化學(xué)鍵合強(qiáng)堿性季銨鹽陰離子交換固定相 簡稱SAX柱L15：已基硅烷化學(xué)鍵合全多孔硅膠微球固定相 簡稱C6柱L16：二甲基硅烷化學(xué)鍵合全多孔硅膠微粒固定相L17：氫型磺化交聯(lián)苯乙烯-二乙烯基苯共聚物,強(qiáng)陽離子交換樹脂L18: 310um全多孔硅膠化學(xué)鍵合胺基（NH2）和氰基（CN）L19

3、：鈣型磺化交聯(lián)苯乙烯二乙烯基苯共聚物，強(qiáng)陽離子交換樹脂L20：二羥基丙烷基化學(xué)鍵合多孔硅膠微球固定相（Diol） 簡稱二醇基柱L21：剛性苯乙烯二乙烯基苯共聚物微球L22：帶有磺酸基團(tuán)的多孔苯乙烯陽離子交換樹脂L23：帶有季胺基團(tuán)的聚甲基丙烯酸甲酯或聚丙烯酸酯多孔離子交換樹脂L24：表面含有大量羥基的半剛性聚乙烯醇親水凝膠L25：聚甲基丙烯酸酯樹脂交聯(lián)羥基醚（表面含有殘余羧基功能團(tuán)）樹脂。能分離分子量1005000MW范圍的水溶性中性、陽離子型及陰離子型聚合物（用聚氧乙烯測定）的固定相L26：丁基硅烷化學(xué)鍵合全多孔硅膠微球固定相L27：3050um的全多孔硅膠微粒L28：多功能載體，100?的

4、高純硅膠加以氨基鍵合以及C8反相鍵合的官能團(tuán)L29: 氧化鋁,反相鍵合,含碳量低,氧化鋁基聚丁二稀小球,5um，孔徑80?L30: 全多孔硅膠鍵合乙基硅烷固定相L31: 季胺基改性孔徑2000?的交聯(lián)苯乙烯和二乙烯基苯(55%)強(qiáng)陰離子交換樹脂L32: L-脯氨酸銅配合物共價鍵合于不規(guī)則形硅膠微粒的配位體的交換手性色譜填料L33: 能夠分離分子量400040000MW范圍蛋白質(zhì)分子的球形硅膠固定相,pH穩(wěn)定性好L34：鉛型磺化交聯(lián)苯乙烯二乙烯基苯共聚物強(qiáng)陽離子交換樹脂，9um球形L35：鋯穩(wěn)定的硅膠微球鍵合二醇基親水分子單層固定相，孔徑150?L36: 5um胺丙基硅膠鍵合L-苯基氨基乙酸3,

5、5二硝基苯甲酰L37：適合分離分子量200040,000Mw的聚甲基丙烯酸酯凝膠L38：水溶性甲基丙烯酸酯基質(zhì)SEC色譜柱L39：親水全多孔聚羥基甲基丙烯酸酯色譜柱L40：Tris 3,5二甲基苯基氨基甲酸酯纖維素涂覆多孔硅膠微球L41：球形硅膠表面固定1酸糖蛋白固定相L42: C8和C18硅烷化學(xué)鍵合多孔硅膠固定相L43: 硅膠微球鍵合五氟代苯基固定相L44: 多功能固定相,60 ?高純硅膠基質(zhì)鍵合磺酸陽離子交換功能團(tuán)和C8反相功能團(tuán)L45: -環(huán)糊精鍵合多孔硅膠微球L46: 季胺基改性苯乙烯-二乙烯基苯聚合物微球液相色譜柱USP column PackingL1 Octadecyl sil

6、ane chemically bonded to porous silica or ceramic micro-particles,3 to 10um in diameterL2 Octadecyl silane chemically bonded to silica gel of a controlled surface porosity that has been bonded to a solid spherical core, 30 to 50um in diameter.L3 Porous silica particles, 5 to 10um in diameter.L4 Sili

7、ca gel of controlled surface porosity bonded to a solid spherical core, 30 to 50um in diameter.L5 Alumina of controlled surface porosity bonded to a solid spherical core , 30 to 50um in diameter.L6 Strong cation exchange packing-sulfonated fluorocarbon polymer coated on a solid spherical, 30 to 50um

8、 in diameter.L7 Octylsilane chemically bonded to totally porous silica particles , 3 to 10um in diameter.L8 An essentially monomolecular layer of aminopropylsilane chemically bonded to totally porous silica gel support, 10um in diameter.L9 10um irregular or spherical layer of aminopropylsilane chemi

9、cally bonded, strongly acidic cation-exchange coating.L10- Nitrile groups chemically bonded to porous silica particles, 3 to 10um in diameter.L11- Phenyl group chemically bonded to porous silica particles, 5 to 10um in diameter.L12- A strong anion-exchange packing made by chemically bonding a quater

10、nary ammonium anion-exchange coating.L13- Trimethylsilane chemically bonded to porous silica particles, 3 to 10um in diameter.L14 Silica gel 10um in diameter having a chemically bonding a quaternary ammonium anion-exchange coating.L15 Hexylsilane chemically bonded to totally porous silica particles,

11、 3 to 10um in diameter.L16 Dimethylsilane chemically bonded to porous silica particles, 5 to 10um in diameter.L17_ Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the hydrogen from, 7 to 11um in diameter.L18 Amion and cyano groups chemically bon

12、ded to porous silica particles, 3 to 10um in diameter.L19 Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divilbenzene copolymer in the calcaium from, about 10um in diameter.L20- Dihydroxypropane groups chemically bonded porous silica particles , 5 to 10um in diameter.L21

13、A rigid, spherical styrene-divinylbenxene copolymer, 5 to 10um in diameter.L22 A cation-exchange resin made of porous polystyrene gel with sulfonic acid group, about 10um in diameter.L23 An cation-exchange resin made of porous polymethacrylate or polyacrylate gel with quaternary ammonium groups, abo

14、ut 10um in size.L24 A semi-right hydrophilic gel consisting of vinyl polymers with numerous hydroxyl groups on the matrix surface , 32 to 63um in diameter.5L25 _Packing having the capacity to separate compounds with a molecular weight range from 100-5000 (as determined by polyethylene oxide), applie

15、d to neutral, and cationic water-soluble polymers. A polymethacrylate resin base, cross-linked with poly-hydroxylated ether (surface contained some residual carboxylfunctional groups) was fund suitable.L26- Butyl silica chemically bonded to totally porous silica particles, 5 to 10um in diameter.L27

16、_ Porous silica particles, 30 to 5um in diameter.L28 A multifunctional support, which consists of a high purity, 100 Å, spherical silica substrate that has been bonded with anionic exchange, amine functionality in addition to a conventional reversed phase C8 functionality.L29_ Gamma alumina, re

17、verse-phase, low carbon percentage by weight, alumina-based polybutadiene, spherical particles. 5um in diameter with a pore volume of 80 ÅL30- Ethyl silica chemically bonded to totally porous silica particles, 3 to 10um in diameter.L31 A strong anion-exchange resin-quaternary amine bonded on la

18、tex particles attached to a core of 8.5um macroporous particles having a pore size of 2000Å and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene.L32 A chiral ligand-exchange packing-L-proline copper complex covalently bonded to irregularly shaped silica particles, 5 to 10um

19、in diameter.L33- Packing having the capacity to separate dextrans by molecular size over a range of 4000 to 500000 Da. It is spherical, silica-based, and processed to provide PH stability.6L34 Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinybenzene copolymer in the l

20、ead form, about 9um in diameter.L35- A zirconium-stabilized spherical silica packing with a hydrophilic(diol-type) molecular monolayer bonded phase having p pore size of 150Å.L36 A 3,5-dinitrobenzoyl derivative of L-phenylglycine covalently bonded to 5um aminopropyl silica.L37 Packing having th

21、e capacity to separate proteins by molecular size over range of 2000 to 40000Da. It is a polymethacrylate gel.L38 A methacrylate-based size-exclusion packing for water-soluble samples.L39- A hydrophilic polyhydroxymethacrylate gel of totally porous spherical resin.L40 Cellulose tris-3,5-dimethylphen

22、ylcarbmate coated porous silica particles, 5 to 20umin diameter.L40Cellulose tris-3,5-dimethylphenylcarbamate coated porous silica particles,5 to 20 m indiameter.L41Immobilized 1 acid glycoprotein on spherical silica particles,5m in diameter.L42Octylsilane and octadecylsilane groups chemically bonde

23、d to porous silica particles, 5m in diameter.L43Pentafluorophenyl groups chemically bonded to silica particles by a propyl spacer, 5 to 10m in diameter.L44A multifunctional support,which consists of a high purity,60Å,spherical silica substrate that has been bonded with a cationic exchanger,sulf

24、onic acid functionalityin addition to conventional reversed phase C8 functionality.L45Beta cyclodextrin bonded to porous silica particles, 5 to 10m in diameter.L46Polystyrene/divinylbenzene substrate agglomerated with quaternary amine functionalized latex beads, 10m in diameter.L47High-capacity anio

25、n-exchange microporous substrate,funlly functionalized with trimethlyamine groups,8m in diameter.7L48Sulfonated,cross-linked polystyrene with an outer layer of submicron,porous,anion-exchange microbends 15m in diameter.L49A reversed-phase packing made by coating a thin layer of polybutadiene onto sp

26、herical porous zirconia particles,3 to 10m in diameter.8L50Multifunction resin with reversed-phase retention and strong anion-exchange functionalities.The resin consists of ethylvinylbenzene 55cross-linked with divinylbenzene copolymer,3 to 15m in diameter,and a surface area not less than 350per g.S

27、ubstrate is coated with quaternary ammonium functionalized latex particles consisting of styrene cross-linked with divinylbenzene.9L51Amylose tris-3,5-dimethylphenylcarbamate-coated,porous,spherical,silica particles, 5 to 10m in diameter.10L52A strong cation exchange resin made of porous silica with

28、 sulfopropyl groups,5 to 10m in diameter.11L53Weak cation-exchange consisting of ethylvinylbenzene,55cross-linked with divinylbenzene copolymer, 3 to 15m in diameter.Substrate is surface grafted with carboxylic acid and/orphosphoric acid functionalized monomers.Capacity not less than 500Eq/column.12

29、L54A size exclusion medium made of covalent bonding of dextran to highly cross-linked porous agarose beads,about 13 m in diameter.13L55A strong cation-exchange resin made of porous silica coated with polybutadiene-maleic acid copolmer,about 5 m in diameter.14L56Isopropyl silane chemically bonded to

30、totally porous silica paryicles,3 to 10m in diameter.15L57A chiral recognition protein,ovomucoid,chemically onded to silica particles,about 5m in diameter,with a pore size of 120ÅL58Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the sodium

31、 form,about7to 11m in diameter,16 L59Packing having the capoacity to separate proteins bymolecular weight over the range of 10 to 500kDa.It is spherical(10m),silica-based, and processed to provide hydrophilic characteristics and pH stability.17USP28 L60Spherical,porous silica gel,3 or 5m in diameter

32、,the surface of which has been covalently modified with palmitamido-propyl groups and endcapped with acetamidopropyl groups to a ligand density of about 6 moles per .18USP28L61 A hydroxide selective strong anion-exchange resin consisting of a highly cross-linked core of 13 µm microporous partic

33、les having a pore size less than 10 Angstrom units and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene with a latex coating composed of 85 nm diameter microbeads bonded with alkanol quartenary ammonium ions (6%)L62 C30 silane bonded phase on a fully porous spherical silica, 3 to

34、 15 µm in diameterL63 Glycopeptide teicoplanin linked through multiple covalent bonds to a 100 Angstrom units spherical silica.L64 Strongly basic anion exchange resin consisting of 8% crosslinked styrene divinylbenzene copolymer with a quartenary ammonium group in the chloride form, 45 to 180 &

35、#181;m in diameter.L65 Strongly acidic cation exchange resin consisting of 8% sulfonated crosslinked styrene divinylbenzene copolymer with a sulfonic acid group in the hydrogen form, 63 to 250 µm in diameter.L66 A crown ether coated on a 5 µm particle size silica gel substrate. The active

36、site is (S)-18-crown-6-ether.L67 Porous vinyl alcohol copolymer with a C18 alkyl group attached to the hydroxyl group of the polymer.L68 Spherical, porous silica, 10 µm or less in diameter, the surface of which has been covalently modified with alkyl amide groups and not endcapped.L69 Ethylviny

37、lbenzene/divinylbenzene substrate agglomerated with quartenary amine functionalized 130 nm latex beads, about 6.5 µm in diameter.L70 Cellulose tris(phenyl carbamate) coated on 5 µm silicaL71 A rigid, spherical, polymethacrylate, 4 to 6 µm in diameterL72 (S)-phenylglycine and 3,5-dinit

38、roanaline urea linkage covalently bonded to silica.L73 A rigid spherical polydivinylbenzene particle, 5 to 10 µm in diameterL74 A strong anion-exchange resin consisting of a highly cross-linked core of 7 µm macroporous particles having a 100 Angstroms average pore size and consisting of et

39、hylvinylbenzene cross-linked with 55% divinylbenzene and an anion-exchange layer grafted to the surface, which is functionalized with alkyl quartenary ammonium ions.氣相色譜柱PhasesG1Dimethylpolysilxane oil.G2Dimethylpolysilxane gum.G350%Phenyl-50% methylpolysiloxane.G4Diethylene glycol succinate polyest

40、er.G53-Cyanopropylpolysiloxane.G6Trifluoropropylmethylpolysiloxane.G750%3-Cyanopropyl-50%phenylmethylsilicone.G880% Bis(3-cyanopropyl)-20% 3-cyanopropylphenylpolysi-loxane(percentages refer to molar substitution).G9Methylvinylpolysiloxane.G10Polyamide formed by reacting a C36 dicarboxylic acid with

41、1,3-di-4-piperidylpropane and piperidine in the respective mole ratios of 1.00:0.90:0.20.G11Bis(2-ethylhexyl)sebacate polyester.G12Phenyldiethanoamine succinate polyester.G13Sorbitol.G14Polyethylene glycol(av.mol.wt.of 950 to 1050).G15Polyethylene glycol(av.mol.wt.of 3000 to 3700).G16Polyethylene gl

42、ycol compound(av.mol.wt.about 15,000).A high molecular weight compound of polyethene glycol with a diepoxide linker.Available commercially as Polyethylene Glycol Compound 20M,or as Carbowax 20M,from suppliers of chromatographic reagents.G1775% Phenyl-25% methylpolysiloxane.G18Polyalkylene glycol.G19

43、25% Phenyl-25% cyanopropyl-50% methylsilicone.G20Polyethylene glycol(av.mol.wt.of 380 to 420).G21Neopentyl glycol succinate.G22Bis(2-ethylhexyl)phthalate.G23Polyethyl glycol adipate.G24Diisodecyl phthalate.G25Polyethylene glycol compound TPA.A high molecular weight compound of a polyethylene glycol

44、and a diepoxide as Carbowax 20M-TPA from suppliers of chromatographic reagents.G2625% 2-Cyanoethyl-75% methylpolysiloxane.G275% Phenyl-95% methylpolysiloxane.G2825% Phenyl-75% methylpolysiloxane.G293,3-Thiodipropionitrile.G30Tetraethylene glycol dimethyl ether.G31Nonylphenoxypoly(ethyleneoxy)ethanol

45、 (av.ethyleneoxy chain length is 30);Nonoxynol 30.G3220% Phenylmethyl-80% dimethylpolysiloxane.G3320% Carborane-80% methylsilicone.G34Diethylene glycol succinate polyester stabilized with phosphoric acid.G35A high molecular weight compound of a polyethylene glycol and a diepoxide that is esterified

46、with nitroterephthalic acid.G361% Vinyl-5% phenylmethylpolysiloxane.G37Polyimide.G38Phase G1 containing a small percentage of a tailing inhibitor.19G39Polyethylene glycol(av.mol.wt.about 1500).G40Ethylene glycol adipate.G41Phenylmethyldimethylsilicone(10% phenyl-substituted).G4235% phenyl-65% dimeth

47、ylpolysiloxane(percentages refer to molar substitution).G436% cyanopropyhenyl-94% dimethylpolysiloxane(percentages refer to molar substitution).G442% low molecular weight petrolatum hydrocarbon grease and 1% solution of potassium hydroxide.G45Divinylbenzene-ethylene glycol-dimethylacrylate.G4614% Cy

48、anopropylphenyl-86% methylpolysiloxane.G47Polvethylene glycol(av.mol.wt.of about 8000).G48Highly polar,partially cross-linked cyanopolysiloxane.G49Proprietary derivatized phenyl groups on a polysiloxane backbone.20SupportsNOTEUnless otherwise specified,mesh sizes of 80 to 100 or,alternatively,100 to 120 are intended.S1ASiliceous earth for gas chromatography has been fluxcalcined by mixing diatom