常用緩沖液配方及緩沖范圍-乙磺酸緩沖液

1、常見緩沖液的緩沖范圍緩沖液名稱及常用濃度緩沖pH范圍主要物質(zhì)分子量MES (2嗎啉代乙磺酸)5.5-6.7195.2Bis-Tris5.8-7.2209.2HEPES6.8-8.2238.3PIPES6.1-7.5302.4MOPS6.5-7.9209.3Tricine7.4-8.8179.2TEA (三乙醇胺)7.4-8.3149.2甘氨酸-鹽酸緩沖液(0.05mol/L)2.2-5.0甘氨酸Mr=75.07鄰苯二甲酸-鹽酸緩沖液(0.05mol/L)2.2-3.8鄰苯二甲酸氫鉀 Mr=204.23磷酸氫二鈉-檸檬酸緩沖液2.2-8.0磷酸氫二鈉 Mr=141.98檸檬酸-氫氧化鈉-鹽酸緩沖

2、液2.2-6.5檸檬酸Mr=192.06檸檬酸-檸檬酸鈉緩沖液(0.1mol/L)3.0-6.6檸檬酸Mr=192.06 檸檬酸鈉Mr=257.96乙酸-乙酸鈉緩沖液(0.2mol/L)3.6-5.8乙酸鈉 Mr=81.76乙酸 Mr=60.05鄰苯二甲酸氫鉀-氫氧化鈉緩沖液4.1-5.9氫鉀 Mr=204.23磷酸氫二鈉-磷酸二氫鈉緩沖液(0.2mol/L)5.8-8.0磷酸氫二鈉-磷酸二氫鉀緩沖液(1/15 molL)4.92-8.18磷酸二氫鉀-氫氧化鈉緩沖液(0.05mol/L)5.8-8.0巴比妥鈉-鹽酸緩沖液(18C )6.8-9.6巴比妥鈉Mr=206.18Tris-鹽酸緩沖液(

3、0.05mol/L 25 C)7.10-9.00三羥甲基氨基甲烷(Tris )Mr=121.14甘氨酸-氫氧化鈉緩沖液(0.05mol/L)8.6-10.6甘氨酸Mr=75.07硼砂-氫氧化鈉緩沖液(0.05mol/L)9.3-10.1硼砂 Na2B4O7 10H2O Mr=381.43碳酸鈉-碳酸氫鈉緩沖液(0.1mol/L)9.16-10.83碳酸鈉Mr=286.2 碳酸氫鈉 Mr=84.0碳酸鈉-氫氧化鈉緩沖液(0.025mol/L)9.6-11.0磷酸氫二鈉-氫氧化鈉緩沖液10.9-12.0氯化鉀-鹽酸緩沖液(0.2mol/L )1.0-2.2氯化鉀Mr=74.55氯化鉀-氫氧化鈉緩沖

4、液(0.2mol/L )12.0-13.0氯化鉀Mr=74.55參考下圖：常見緩沖液的緩沖范圍常用緩沖液的配制方法1.甘氨酸-鹽酸緩沖液（0.05M ）X ml 0.2M 甘氨酸 +Y ml 0.2M 鹽酸 再加水稀釋至 200mlpHX/mlY/mlpHX/mlY/ml2.25044.03.05011.42.45032.43.2508.22.65024.23.4506.42.85016.83.6505.0甘氨酸分子量=75.070.2M甘氨酸溶液含15.01g/L2.鄰苯二甲酸-鹽酸緩沖液（0.05M）X ml 0.2M 鄰苯二甲酸氫鉀 +Y ml 0.2M 鹽酸 再加水稀釋至 200mlp

5、HXYpHXY2.254.6703.251.4702.453.9603.450.9902.653.2953.650.5972.852.6423.850.2633.052.032鄰苯二甲酸氫鉀分子量=2.4.230.2M鄰苯二甲酸氫鉀溶液含40.85g/L3.磷酸氫二鈉-檸檬酸緩沖液pH0.2MNa2HPO4/ml0.1M檸檬酸/mlpH0.2MNa2HPO4/ml0.1M 檸檬 酸/ml2.20.4019.65.210.729.282.41.2418.765.411.158.852.62.1817.825.611.608.402.83.1716.835.812.097.913.04.1115.

6、896.012.637.373.24.9415.066.213.226.783.45.7014.306.413.856.153.66.4413.566.614.555.453.87.1012.906.815.454.554.07.7112.297.016.473.534.28.2811.727.217.392.614.48.8211.187.418.171.834.69.3510.657.618.731.274.89.8610.147.819.150.855.010.309.708.019.450.550.2M 溶液含 28.40g/L0.2M 溶液含 35.61g/L0.1M 溶液含 21.

7、01g/LNa2HPO4 分子量=141.98Na2HPO4 2H2O 分子量=178.05C6H8O7 H2O 分子量=210.144.檸檬酸-氫氧化鈉-鹽酸緩沖液pH鈉離子濃度/M檸檬酸C6H8O7 H2O/g氫氧化鈉NaOH/g濃鹽酸HCl/ml終體積/L2.20.2021084160103.10.2021083116103.30.2021083106104.30.202108345105.30.3524514468105.80.45285186105106.50.3826615612610使用時可以每升中加入 1g酚，若最后pH有變化，再用少量 50%氫氧化鈉溶液或濃鹽酸調(diào) 節(jié)，冰箱保

8、存。5.檸檬酸-檸檬酸鈉緩沖液（0.1M ）pH0.1M檸檬酸/ml0.1M檸檬酸 鈉/mlpH0.1M檸檬酸/ml0.1M檸檬酸 鈉/ml3.018.61.45.08.211.83.217.22.85.27.312.73.416.04.05.46.413.63.614.95.15.65.514.53.814.06.05.84.715.34.013.16.96.03.816.24.212.37.76.22.817.24.411.48.66.42.018.04.610.39.76.610418.64.89.210.8C6H8O7 H2O 分子量=210.140.1M 溶液含 21.01g/LNa

9、3C6H5O7 2H2O 分子量=294.120.1M 溶液含 29.41g/L6.乙酸-乙酸鈉緩沖液（0.2M）pH0.2MNaAc/ml0.2MHAC/mlpH0.2MNaAc/ml0.2MHAC/ml3.60.759.254.85.904.103.81.208.805.07.003.004.01.808.205.27.902.104.22.657.355.48.601.404.43.706.305.69.100.904.64.905.105.89.400.60NaAc 分子量=136.090.2M 溶液為 27.22g/L7.磷酸鹽緩沖液（1）磷酸氫二鈉-磷酸二氫鈉緩沖液（0.2M）pH

10、0.2MNa2HPO4/ml0.2MNaH2PO4/mlpH0.2MNa2HPO4/ml0.2MNaH2PO4/ml5.88.092.07.061.039.05.910.090.07.167.033.06.012.387.77.272.028.06.115.085.07.377.023.06.218.581.57.481.019.06.322.577.57.584.016.06.426.573.57.687.013.06.531.568.57.789.510.56.637.562.57.891.58.56.743.556.57.993.07.06.849.551.08.094.75.36.95

11、5.045.00.2M 溶液含 35.61g/L0.2M 溶液含 71.64g/L0.2M 溶液含 27.6g/L0.2M 溶液含 31.21g/LNa2HPO4 2H2O 分子量=178.05Na2HPO4 12H2O 分子量=358.22NaH2PO4 H2O 分子量=138.01NaH2PO4 2H2O 分子量=156.03磷酸氫二鈉-磷酸二氫鉀緩沖液（1/15M ）pH1/15MNa2HPO4/ml1/15MKH2PO4/mlpH1/15MNa2HPO4/ml1/15MKH2PO4/ml4.920.109.907.177.003.005.290.509.507.388.002.005.

12、911.009.007.739.001.006.242.008.008.049.500.506.473.007.008.349.750.256.644.006.008.679.900.106.815.005.009.1810.0006.986.004.00Na2HPO4 2H2O 分子量=178.051/15M 溶液含 35.61g/LKH2PO4 分子量=136.091/15M 溶液含 9.078g/L8.磷酸二氫鉀-氫氧化鈉緩沖液（0.05M ）X ml 0.2M KH2PO4 +Y ml 0.2M NaOH再加水稀釋至 20mlpH(20 C)X/mlY/mlpH(20 C)X/mlY/

13、ml5.850.3727.052.9636.050.5707.253.5006.250.8607.453.9506.451.2607.654.2806.651.7807.854.5206.852.3658.054.6809.巴比妥鈉-鹽酸緩沖液（18C）pH0.04M 巴比 妥鈉/ml0.2M 鹽酸 /mlpH0.04M 巴比 妥鈉/ml0.2M 鹽酸 /ml6.810018.48.41005.217.010017.88.61003.827.210016.78.81002.527.410015.39.01001.657.610013.49.21001.137.810011.479.41000.

14、708.01009.399.61000.358.21007.21巴比妥鈉分子量=206.180.04M 溶液為 8.25g/L10. Tris-鹽酸緩沖液50ml 0.1M三羥甲基氨基甲烷（Tris）溶液于X ml 0.1M鹽酸混勻后，加水稀釋至100mlpHX/mlpHX/ml7.145.78.126.27.244.78.222.97.343.48.319.97.442.08.417.27.540.38.514.77.638.58.612.47.736.68.710.37.834.58.88.57.932.08.97.08.029.2三羥甲基氨基甲烷（Tris）分子量=121.140.1M溶

15、液為12.114g/LTris溶液可以從空氣中吸收二氧化碳，使用時注意將瓶蓋嚴11.硼酸-硼砂緩沖液（0.2M硼酸根）pH0.05M硼砂/ml0.2M 硼酸 /mlpH0.05M硼砂/ml0.2M 硼酸 /ml7.41.09.08.23.56.57.61.58.58.44.55.57.82.08.08.76.04.08.03.07.09.08.02.0硼砂 Na2B4O7 10H2O 分子量=381.430.05M（=0.2M 硼酸根）溶液為 19.07g/L硼酸H3BO3分子量=61.840.2M溶液為12.37g/L硼砂易失去結(jié)晶水，必須在帶塞的瓶中保存12.甘氨酸-氫氧化鈉緩沖液（0.0

16、5M ）X ml 0.2M 甘氨酸+Y ml 0.2M 氫氧化鈉 再加水稀釋至 200mlpHX/mlY/mlpHX/mlY/ml8.6504.09.65022.48.8506.09.85027.29.0508.810.05032.09.25012.010.45038.69.45016.810.65045.513.硼砂-氫氧化鈉緩沖液（0.05M硼酸根）X ml 0.05M硼砂+Y ml 0.2M 氫氧化鈉 再加水稀釋至 200mlpHX/mlY/mlpHX/mlY/ml9.3506.09.85034.09.45011.010.05043.09.65023.010.15046.0硼砂 Na2B4O7 10H2O 分子量=381.430.05M（=0.2M 硼酸根）溶液為 19.07g/L14.碳酸鈉-