簡(jiǎn)介Waters UPLC故障排除

1、2013 Waters Corporation 12013 Waters Corporation 2Waters UPLC Troubleshooting Training 2013 Waters Corporation 3ACQUITY UPLC Family ：UPLC & H-ClassWaters UPLC SystemUPLC Solvent Management (BSM)UPLC Sample Management (SM)Waters ACQUITY H-Class SystemQuaternary Solvent Manager (QSM) Sample Manage

2、r-Flow Through Needle (SM-FTN)Waters UPLC Detector Troubleshooting TrainingWaters UPLC PDA and PDAeTUVFLRELSDOpen Discussion2013 Waters Corporation 42013 Waters Corporation 52013 Waters Corporation 6Detectors:Optical and/or Mass SpecTunable UV or Photodiode ArrayOptimized flow cell for UPLCHigh spee

3、d data samplingColumn Manager:Innovative pivot designPositions column to detectorE-Cord connectionSample Manager:Low dispersion XYZZ FormatFast cycle timesLow carryoverPlates and/or vialsOptional Sample OrganizerBinary Solvent Manager:High pressure blendingBinary gradientsFour solvent choicesOn-line

4、 degassingLow dispersion designUPLC pressure capabilitiesSample Organizer: (option)Expands capacity (22/15/8)Shuttles plate feedHeated/chilledSystem Considerations:Small FootprintRedesigned tubing and fittingsConsolidated waste managementIntegrated system diagnosticsConnections Insight remote diagno

5、stics2013 Waters Corporation 7Waste Tubing management Chromatographic solvents:oA1/A2, B1/B2Two Sample Manager wash solventsSeal Wash Common fittings and tools for usersColumn inlets/outlets and fittings oFinger tight, turn (use 1/4” wrench)Dedicated tubing3 piece compression screw and ferrule Small

6、 footprint 13” wide24.25” with Sample Organizer2013 Waters Corporation 82013 Waters Corporation 9 15 K psi performance Accurate and precise flow rates Accurate and precise composition Low delay volume for gradient applicationso5% Ethyl AcetateToluene Chlorinated solvents: (Trichlorobenzene) 2013 Wat

7、ers Corporation 152013 Waters Corporation 16 Pressure Transducer Valve Manifold Drive Gears Wash SyringesWeak Wash Strong WashMetering Syringe Drive Assembly 2013 Waters Corporation 17 Metering Syringe Valve3 Way Rotary Valve Sample Syringe50 uL volume PN 410001348100 L volume PN 700002570250 L volu

8、me PN 410001347o for use with 50 L loop2013 Waters Corporation 182013 Waters Corporation 19192013 Waters Corporation 202013 Waters Corporation 212013 Waters Corporation 222013 Waters Corporation 232013 Waters Corporation 24Description P/NStatus1 L Loop430001516Optional2 L Loop430001264Optional5 L Lo

9、op430001233Optional10 L Loop430001326Standard20 L Loop430001320Optional 50 L Loop430001325Optional100 L Loop170001792(Kit) Optional250 L Loop176001780(Kit) Optional2013 Waters Corporation 25Before running the Characterize Needle and Loop Volume function, make sure the proper volumes are configured i

10、n the Volumes Configuration menu. The above values are standard for currently shipping ACQUITY system.2013 Waters Corporation 26Loop Volume (uL)Partial Loop w/Needle Overfill Mode Recommended sample injection volume range (uL)Partial Loop (Pressure Assist Mode) Recommended sample injection volume ra

11、nge (uL)Full Loop Mode Recommended sample injection volume (uL0Automatic overfill factor1uL0.2 to 0.75Not Recommended1.05.82uL0.4 to 1.5Not Recommended2.05.65uL1.0 to 3.8Not Recommended5.0510uL2.0 to 7.52.0 to 5.010.0420uL4.0 to 15.04.0 to 10.020.0350uL10.0 to 37.510.0 to 25.050.02100uL20.0 to 75.02

12、0.0 to 50.0100.02250uL40.0 to 150.040.0 to 125.0250.01.5Recommended PLNO injection volume 20% to 75% of loop volumeRecommended Partial Loop Injection 20% to 50% of loop volume2013 Waters Corporation 27QSMQuaternary Solvent Manager SM-FTNSample Manager-Flow Through Needle CH-AHigh Temperature Column

13、HeaterActive Pre-Heating DetectorsTUV, PDA ePDA, FLR, ELSD2013 Waters Corporation 28The ACQUITY H-Class System consists of the following:Quaternary Solvent Manager (QSM)Four solvent low pressure gradient mixing Supports UPLC PressuresLow gradient delay volumeSM-FTN (Sample Manager-Flow Through Needl

14、e)One injection mode- direct inject typeMuch simpler- Just specify injection volumeExcellent recovery and precisionCH-AHigh temperature column heater with active pre-heating2013 Waters Corporation 29Primary HeadAccumulator HeadVent Valve100ul MixerGradient Proportioning Valve PairsLow PressureManifo

15、ldDegasser ChambersSeal Wash Pump2013 Waters Corporation 302013 Waters Corporation 312013 Waters Corporation 322013 Waters Corporation 33Metering Syringe ValveCheck Valve and UnionInject ValveWash Solvent Inlet Line (not degassed)Leak Sensor ConnectionsSample Metering Syringe2013 Waters Corporation

16、34To Check ValveTo CH-A and ColumnStationary Side TubeFrom PumpTo Pressure TransducerTo Sample Needle2013 Waters Corporation 35Power and Status LEDsPlate Selector SwitchRotary Tray(Plate Holder)Magnetic Door SensorPower Switch2013 Waters Corporation 362013 Waters Corporation 37Improved Door GasketAd

17、ded Tubing Gasket (top and bottom)For 3rd party Mass Spec at left side ofACQUITY Stack.Allows use of existingPassive Pre-heaterNo wires to connectActive Pre-heater2013 Waters Corporation 382013 Waters Corporation 39Gold Plated Compression ScrewPEEK FerruleStainless Steel Cap NutStainless Steel Tubing (with brazed on collet)2013 Waters Corporation 402013 Waters Corporation 41Supports all current ACQUITY detectorsPDA and PDAeTUVFLRELSD2013 Waters Corporat