Brefeldin-A-BFA-DataSheet-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEBrefeldin ACat. No.: HY-16592CAS No.: 20350-15-6Synonyms: BFA; Cyanein; Decumbin分式: CHO分量: 280.36作靶點(diǎn): Autophagy; CRISPR/Cas9; Mitophagy作通路: Autophagy; Cell Cycle/DNA Damage儲存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 mont

2、hs-20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 20.83 mg/mL (74.30 mM; Need ultrasonic)Mass Solvent1 mg 5 mg 10 mg Concentration制備儲備液1 mM 3.5668 mL 17.8342 mL 35.6684 mL5 mM 0.7134 mL 3.5668 mL 7.1337 mL10 mM 0.3567 mL 1.7834 mL 3.5668 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存式和期限。體內(nèi)實(shí)驗(yàn)請根據(jù)您的實(shí)驗(yàn)動物和給藥式選擇適當(dāng)?shù)娜芙獍?，配制前請先配制澄?/p>

3、的儲備液，再依次添加助溶劑(為保證實(shí)驗(yàn)結(jié)果的可靠性，體內(nèi)實(shí)驗(yàn)的作液，建議您現(xiàn)現(xiàn)配，當(dāng)天使；澄清的儲備液可以根據(jù)儲存條件，適當(dāng)保存；以下溶劑前的百分 指該溶劑在您配制終溶液中的體積占)：1. 請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.08 mg/mL (7.42 mM); Clear solution2. 請依序添加每種溶劑： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.08 mg/mL (7.42 mM); Clear solution3. 請依序添

4、加每種溶劑： 10% DMSO 90% corn oil1/3 Master of Small Molecules 您邊的抑制劑師www.MedChemESolubility: 2.08 mg/mL (7.42 mM); Clear solutionBIOLOGICAL ACTIVITY物活性 Brefeldin A蛋質(zhì)運(yùn)輸 (protein trafficking) 的特異性抑制劑，其阻斷蛋質(zhì)從內(nèi)質(zhì)向爾基復(fù)合體的轉(zhuǎn)運(yùn)。IC50 & Target CRISPR/Cas9體外研究 Brefeldin A treatment for 15 h or 40 h, causes dramatic sw

5、elling of the Endoplasmic Reticulum (ER) andshifts its localization to the periphery of normal rat kidney (NRK) cells. Prolonged Brefeldin A treatmentresults in marked disruption of the MT and actin cytoskeleton 1. ADP-ribosylation of BARS is mediated byformation of a conjugate between Brefeldin A a

6、nd ADPR. BARS shows BAC binding when incubated with themedium from the BFA-treated CD38+ HeLa cells 2. Brefeldin A induces anchorage-independent cell deathin MDA-MB-231 breast cancer cells, inhibits the formation of MDA-MB-231 colonies in 3D and 2D culturesand inhibits the migration and MMP 9 (Matri

7、x Metallopeptidase 9) activity of MDA-MB-231 3.PROTOCOLCell Assay 1 Cells are grown on glass coverslips, fixed in 3 % paraformaldehyde in PBS (10 min at room temperature) andthen washed in PBS. Cells are permeabilized with 0.01 % Triton X-lOO in PBS at room temperature for 7min. The coverslips are w

8、ashed (3 times in PBS/0.2 % Tween) incubated in PBS/O.4 % fish skin gelatin/0.2% Tween (5 min) and in PBS/2.5 % goat serum/0.2 % Tween (5 min.). After blocking, the cells are incubatedwith primary antibodies for 45 min at 37C, and then washed with PBS/0.2 % Tween (5 times, 5 min each).The secondary

9、antibodies are added for 30 min at 37C and then cells are washed as above. Coverslips aremounted on slides in 9: 1 glycerol/PBS with 0.1 % o-phenylenediamine.MCE has not independently confirmed the accuracy of these methods. They are for reference only.戶使本產(chǎn)品發(fā)表的科研獻(xiàn) J Autoimmun. 2019 May;99:39-47. Cel

10、l Death Dis. 2018 Nov 16;9(12):1143. FASEB J. 2019 Apr;33(4):5520-5534. J Cell Mol Med. 2019 Apr;23(4):2399-2409. Int J Nanomedicine. 2018 Jan 22;13:479-492.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Alvarez C, et al. Brefeldin A (BFA) disrupts the organization of the micr

11、otubule and the actin cytoskeletons. Eur J Cell Biol. 1999Jan;78(1):1-14.2/3 Master of Small Molecules 您邊的抑制劑師www.MedChemE2. Colanzi A, et al. Molecular mechanism and functional role of brefeldin A-mediated ADP-ribosylation of CtBP1/BARS. Proc Natl Acad SciU S A. 2013 Jun 11;110(24):9794-9.3. Tseng

12、CN, et al. Brefeldin A reduces anchorage-independent survival, cancer stem cell potential and migration of MDA-MB-231 humanbreast cancer cells. Molecules. 2014 Oct 29;19(11):17464-77.4. Wang J, et al. Erythroleukemia cells acquire an alternative mitophagy capability. Sci Rep. 2016 Apr 19;6:24641.5. Yu C, et al. Small molecules enhance CRISPR genome editing in pluripotent stem cells. Cell Stem Cell. 2015 Feb 5;16(2):142-7.McePdfHeightCaut