Real-Thiol _Dye Reagent - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEReal ThiolCat. No.: HY-108715分式: CHNO分量: 411.36作靶點: Others作通路: Others儲存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實驗 DMSO : 101 mg/mL (245.53 mM)* means soluble, but saturation unknown.Mass Solven

2、t1 mg 5 mg 10 mg Concentration制備儲備液1 mM 2.4310 mL 12.1548 mL 24.3096 mL5 mM 0.4862 mL 2.4310 mL 4.8619 mL10 mM 0.2431 mL 1.2155 mL 2.4310 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存式和期限。BIOLOGICAL ACTIVITY物活性 Real Thiol種 于可逆反應(yīng)的熒光探針，可以定量監(jiān)測活細(xì)胞中胱肽的實時動態(tài)變化。體外研究Real Thiol (RT) shows ratiometric fluorescence

3、 responses with a wide dynamic range when reacting withGlutathione (GSH). Real Thiol and its GSH adduct (RT-GSH) shows fluorescence maxima at 487 and 562nm with excitation wavelengths at 405 and 488nm, respectively. The Kd for the reaction between Real Thioland GSH is 3.7mM. Real Thiol has much impr

4、oved quantum yields and photostability. The wide dynamicrange of Real Thiol allows for monitoring of the GSH level changes within 1 to 10mM in both directions in1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemEliving cells. Gel permeation chromatography (GPC) analysis showes that 10% of Real Thiol r

5、eacts withthiolated proteins while 90% of Real Thiol reacts with GSH 1.PROTOCOLKinase Assay 1 Real Thiol (RT) stock solution is diluted with PBS to the desired concentrations. Equal volumes (typically 20to 100L) of various Glutathione (GSH) solutions with different concentrations are mixed with the

6、Real Thiolsolution 10 to 15min before measurement. All samples are prepared on one 96-well/384-well plate with 3replicates each. The plate reader is set to read absorption of all samples first. Fluorescent signals are thenrecorded at ex=405 nm, em=485nm, and ex=488nm, em=565nm with bottom read. For

7、testingfluorescent interference by environmental factors, the Real Thiol stock solution is directly diluted withcorresponding solutions (for example, certain pH buffer or glycerol solution) and measured using the platereader with the same settings stated above 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Jiang X, et al. Quantitative real-time imaging of glutathione. Nat Commun. 2017 Jul 13;8:16087.McePdfHeightCaution: Product has not been fully validated for medical applications.For re