實驗6 兔血清中茶堿的高效液相色譜法測定

1、Experiment Determination of theophylline in rabbit plasma by HPLC18/2/20221. Purpose (1) To learn the serum sample treatment method and to be familiar with the blood sample collection method(2) To learn the experiment on the determination of theophylline in rabbit serun by HPLC28/2/20222. Introducti

2、on Theophylline CaffineNONH3CNNOCH3H38/2/20223. Procedure and Method (1) Preparation of reference standards solutionTheophylline （THP）standard solution: 1.0 mg/mL(2) Preparation of internal standard solution Caffeine (CAF): 1.0 g/mL (Prepared already)(Prepared already)48/2/2022(3) Standard solution(

3、Prepared already)50.0, 125.0, 250.0, 500.0 and 1000.0 g/mL(i) Theophylline standard solution: (ii) Theophylline serum sample prepareartion58/2/2022(3) Standard solution(Prepared already)0, 50.0, 125.0, 250.0, 500.0 and 1000.0 g/mL(i) Theophylline standard solution: (ii) Theophylline serum sample pre

4、paration68/2/2022The calibration curve was constructed by plotting the peak area ratios of each analyte to the IS versus the concentration using weighed linear least square regression.(4) Calibration curve 78/2/2022(5)HPLC conditions Column:ODS column (5m, 150mm4.6mm i.d)Mobile phase：VCH3OH/VH2O=35/65 Flow rate: 1.0 mL min-1Wavelength: 254 nmInjection volumn: 20 L 溫馨提示：高效液相色譜在本層樓的東側403房間 結束后值日生記得清掃衛(wèi)生哦！88/2/2022ResultsCalibration curve , the linear equation and RNO.CTHPTHPCAFATHP/ACAFtRAtRA空白1234598/2/20224. Attention 108/2/20