自動化產(chǎn)品線-核酸提取專題

1、LST 自動化產(chǎn)品線2015核酸專題王中禹2015.04什么是自動化產(chǎn)品？自動化產(chǎn)品有哪些價值？我們的自動化產(chǎn)品有哪些（三大系列）？我們有哪些競爭對手？What is Lab Automation?3加樣機械臂移液分液工具工作臺面（擺放樣品管/試劑/tip等）控制電腦及軟件抓扳手臺面功能模塊溫控（水浴.金屬?。┱袷帲╲ortex）抽濾（離心機）磁珠分離器Plate:HandlerStorage（存放tip、樣品板）儀器室（擺放大型儀器如MMD、PCR/QPCR/GX/HCS等）什么是自動化產(chǎn)品？Automated Liquid Handling Workstation顧名思義自動化（autom

2、ation）：是指在實驗室中某些工作不需要實驗工作者而是以無人值守的方式通過儀器設(shè)備來完成。液體處理（Liquid Handling）：在生命科學(xué)領(lǐng)域的研究中，幾乎所有實驗都是通過對液體進行操作來完成的，具體操作包括：吸液，排液，混合，轉(zhuǎn)移等等。工作站（Workstation）：做實驗的地方或做某種實驗特定步驟的空間，一個工作平臺，具體比如：實驗臺，無菌臺，暗室，冷室，無菌室等。自動化液體處理工作站 自動完成 對液態(tài)實驗品的系列操作 的工作平臺。提問：什么叫自動化核酸提取工作站？自動化產(chǎn)品有哪些價值？標準化重復(fù)性精準度高通量自動化我們的自動化產(chǎn)品有哪些？（老PE自動化）NGS ExpressJ

3、ANUS系列JANUS Chemagic我們的自動化產(chǎn)品有哪些？（Caliper自動化）2007 Zephyr靈活型2009 Zephyr MBW2011 Zephyr NGS Sciclone NGSChemagen核酸提取儀Chemagen核酸提取儀有兩款：chemagic 360可以從血液、血清/血漿、拭子、唾液、排泄物等多種樣本中制備核酸，是核酸提取的完美自動化解決方案。chemagic 360chemagic Prepitochemagic Prepito采用chemagen磁棒微珠分離技術(shù)，是一臺處于頂尖水平的桌面型樣本制備設(shè)備。自動化產(chǎn)品發(fā)展理念的變革配置組合型：根據(jù)應(yīng)用要求，組

4、合各種功能模塊，通過各種必要配件的合理搭配，最終設(shè)計成滿足客戶應(yīng)用的機型。應(yīng)用型：解決典型實驗需求的專用型工作站，按照市場上最有需求的，未來最有潛力的應(yīng)用方向，專門為此應(yīng)用設(shè)計出來單貨號產(chǎn)品。配置組合型工作站JANUS系列JANUS 系列 JANUS Mini MDT JANUS Expanded Deck with Gripper Integration Platform JANUS Standard DeckJANUS Integrator JANUS Mini VarispanJANUS Expanded Deck with MDT JANUS Mini MDT with PlateSt

5、ak Mini 9 Deck Position Standard 24 Deck Position Integrator 24-32 Deck Position Expanded 32 Deck Position 4 8 Tips Varispan Arm 2 Arm Integration with external device 96 - 384 Tips MDT ArmVersaTipTechnology 0.5L 5mLTile - Concept4 Deck capacity3 Arm configurationMDT TechnologyDynamic volume rangefr

6、om 2nL (PinTool)to 235 uL (96Tips P235)JANUSwith Varispanfrom Vialsto Tubesto PlatesEnables multi-tipped processing regardless of labware Automatic variable tip spacing9 to 20 mm (4 tips)9 to 40 mm (8 tips)Tips automatically process sequentially if outside of Varispan limits with no additional progr

7、amming各移液通道可獨立控制+間距可調(diào)以適應(yīng)不同的液體處理formate及實驗器具VersaTip PlusOn a Single Adapter Disposable Tips Fixed, Washable TipsUse Disposable tips to ensure zero carry-overUse fixed, washable tips for buffer or reagent additionNo changing of hardware, no user-intervention required自由切換使用固定鋼針或一次性吸頭根據(jù)所處理液體性質(zhì)平衡防污染與降成本

8、AccuSense Liquid Level SensingAble to sense ionic, and non-ionic liquids including organic solvents such as DMSO or methanolHigh, medium, or low sensitivity settingsRequires minimal liquid in labwareLess than 100 L in a tubeLess than 50 L in a microplate wellIndependent liquid level sensing on each

9、tip for optimal precision and minimal sample carry-overJANUS MDT Head96通道移液頭:I235/96 MDT P235吸頭，5-235 ul，50ul:CV1%I50/96 MDT， P50吸頭，0.5-50ul，5ul：CV1% P20吸頭，0.5-20ul，1ul：CV2%384通道移液頭I30/384 MDT P30吸頭，0.5-30ul，1ul：CV5% P10吸頭，0.5-10ul，1ul：CV5%NanoHead：細鋼針，50nl 1ul，500nl：CV5%Pin tool專利技術(shù)自動在桌面上裝載/卸載不同的移液

10、頭，完全軟件控制可選配MDT Gripper（裝在MDT臂上的小型抓取機械手）做抓取動作MDT 專利技術(shù)JANUS with Gripper Integration PlatformGripper capable of moving plates around deck and to/from peripheral devices such as:Magnetic bead seperator / Vacuum manifoldReadersWashersIncubatorsand much moreComplete assay automation from sample preparati

11、on to results generationIntegrated or Auxiliary Gripper arm optionsGripper Specifications:Top GripperClose to GripOpen to Grip (Protection Pins)355 Rotational CapabilityJANUS: Modular Deck DesignFlexible deck layoutRotate labware at any timeEasier protocol definition and programmingLabware portabili

12、ty across deck and between instrumentsModular Deck DesignThe Tile ConceptJANUS: 功能模塊 JANUS Liquid Handling SoftwareJANUS SoftwareDual Purpose GUIWinPREP Protocol Set-UpOpen & IntuitiveFull ControlKey / Power UsersJANUS Application AssistantRun ProtocolsVery Intuitive GuidanceNo BarriersNo Settings A

13、djustmentsMulti-User EnvironmentWinPREP 4.8 Programming InterfaceDeck Grid and Labware OverviewProtocol Tree and SpecificationsQuick Buttons for Intuitive ProgrammingRight mouse click:Add labware on deck (predefined labware library)Flush and Wash StationFlush and Wash StationReagent TroughFlush and

14、Wash StationReagent Trough96-well PlateJANUS Application Assistant User InterfaceCan be even converted into CHINESESelect Protocol From ListAnswer Protocol Related Questions123Shelf X Y Z4Gather Required Labware with HELP on. Item and Visual Quantity Needed Lab Location Purchasing LinkPlace Labware

15、on DeckCheck the ListProtocol Progress: follow the stepsStatus VisualColor CodedTime Status showing Start and Estimated CompletionTo Ensure a FRESH START All Over配置組合型工作站Cell Explorer上海東方肝膽醫(yī)院睿智化學(xué)中國科學(xué)院動物所北京?？司S爾有限公司應(yīng)用領(lǐng)域分子生物學(xué)PCR Set-upDNA濃度歸一化核酸提取及純化基因組學(xué)NGS 庫制備測序反應(yīng)前處理多重基因表達蛋白質(zhì)組學(xué)蛋白純化MALDI點靶膠內(nèi)酶解蛋白生物標記物分析

16、藥物篩選樣品處理化合物稀釋板復(fù)制化合物庫管理細胞分析高內(nèi)涵篩選RNAi文庫篩選GPCR分析細胞代謝檢測臨床應(yīng)用分子診斷內(nèi)分泌學(xué)檢測感染性疾病檢測免疫學(xué)檢測醫(yī)學(xué)檢測血凝檢測食品檢驗血樣分析JANUS自動化工作站是一自動化液體處理系統(tǒng)，對基因組學(xué)、蛋白質(zhì)組學(xué)、分子診斷、藥物研發(fā)、法醫(yī)學(xué)、化學(xué)等領(lǐng)域?qū)嶒灢僮骱统Ｒ?guī)液體處理等實現(xiàn)自動化。應(yīng)用型工作站 NGS（第二代測序）NGS Express2011 Zephyr NGS Sciclone NGS專門為NGS應(yīng)用而生的硬件設(shè)計預(yù)置NGS流行應(yīng)用方案的金標準程序LabChip XT System Automated DNA Fractionation a

17、nd Sizing (low throughput, high accuracy)Zephyr NGS Workstation LabChip GX (Touch) System HT Bioanalyzer-like QC for gDNA, DNA and RNA, ng & pg sensitivitySciclone NGS Workstation Automated High-throughput Sequencing Sample PreparationMBWJANUS & chemagenDSXTGXNGSXTGXNGSWGeospizaZephyr Molecular Biol

18、ogy Workstation (MBW) JANUS Solutions and Chemagen Nucleic Acid ExtractionLabChip DS System UV-Vis DNA/RNA QuantificationSequencing ServiceBENEFITS:Smaller samples Reduced PCR biasGreater productivityInformatics correlation linkagesGeospiza LIMS Sample TrackingDiseaseResearchFragment& SizingGenerate

19、LibraryQuantifySampleSequenceAnalyzeBetter quality Higher throughput Lower costsJANUS NGS Express Workstation 應(yīng)用型工作站 分子生物學(xué)（核酸提取）2011 Zephyr MBW 靈活的樣品體積可以處理 10 L 到 4 mL的樣品體積簡化的提取流程 獨立的液體處理和提取流程安排：初始樣品轉(zhuǎn)移、濃度歸一化和 PCR 反應(yīng)建立應(yīng)用程序。96 磁頭每次可運行最多 192 個樣品24 磁頭每次可運行最多 48 個樣品最高的核酸產(chǎn)量和純度比其他自動化提取平臺高出兩倍的通量和高出四倍的產(chǎn)量與其他市售平臺相比更低的總處理時間JANUS Chemagic 靈活多用