胰腺的發(fā)育專題講座培訓(xùn)課件

1、胰腺的發(fā)育專題講座胰腺的發(fā)育專題講座The Development of PancreasHelena EdlundE8E10胰腺的發(fā)育專題講座2The Development of PancreasHelThe Development of PancreasHelena Edlund胰腺的發(fā)育專題講座3The Development of PancreasHelThe Structure of PancreasHelena Edlund胰腺的發(fā)育專題講座4The Structure of PancreasHelenTranscriptional SpecificationMicheal Ge

2、rman胰腺的發(fā)育專題講座5Transcriptional SpecificationMForegutPSC(PDX-1+)Pancreatic DuctsPancreas development in the embryo胰腺的發(fā)育專題講座6ForegutPSCPancreatic DuctsPancForegutPSC(PDX-1+)Pancreatic DuctsPancreas development in the embryo胰腺的發(fā)育專題講座7ForegutPSCPancreatic DuctsPancForegutPSC(PDX-1+)Pancreatic DuctsAciniP

3、ancreas development in the embryo胰腺的發(fā)育專題講座8ForegutPSCPancreatic DuctsAcinForegutPSC(PDX-1+)Pancreatic DuctsAciniPancreas development in the embryo胰腺的發(fā)育專題講座9ForegutPSCPancreatic DuctsAcinForegutPSC(PDX-1+)Pancreatic DuctsAciniIsletsPancreas development in the embryo胰腺的發(fā)育專題講座10ForegutPSCPancreatic Duc

4、tsAcinQuestionAre pancreatic stem cells unipotent or multipotent?胰腺的發(fā)育專題講座11QuestionAre pancreatic stem ForegutPSC(PDX-1+)Pancreatic Ducts胰腺的發(fā)育專題講座12ForegutPSCPancreatic Ducts胰腺的發(fā)ForegutPSC(PDX-1+)Pancreatic Ducts胰腺的發(fā)育專題講座13ForegutPSCPancreatic Ducts胰腺的發(fā)ForegutPSC(PDX-1+)Pancreatic Ducts胰腺的發(fā)育專題講座14F

5、oregutPSCPancreatic Ducts胰腺的發(fā)ForegutPSC(PDX-1+)Pancreatic Ducts胰腺的發(fā)育專題講座15ForegutPSCPancreatic Ducts胰腺的發(fā)ForegutPSC(PDX-1+)Pancreatic Ducts胰腺的發(fā)育專題講座16ForegutPSCPancreatic Ducts胰腺的發(fā)ForegutPSC(PDX-1+)Pancreatic Ducts胰腺的發(fā)育專題講座17ForegutPSCPancreatic Ducts胰腺的發(fā)ForegutPSC(PDX-1+)Pancreatic Ducts胰腺的發(fā)育專題講座18F

6、oregutPSCPancreatic Ducts胰腺的發(fā)ForegutPSC(PDX-1+)Pancreatic Ducts胰腺的發(fā)育專題講座19ForegutPSCPancreatic Ducts胰腺的發(fā)Adult Insulin- and Glucagon-Producing Cells Differentiate From Two Independent Cell LineagesPedro Luis HerreraDevelopment 127, 2000Paper One胰腺的發(fā)育專題講座20Adult Insulin- and Glucagon-PrIslet Precursor

7、 Cell Labeling胰腺的發(fā)育專題講座21Islet Precursor Cell Labeling胰Transcriptional STOPBrian Sauer胰腺的發(fā)育專題講座22Transcriptional STOPBrian SaueThe Beta Cell Lineage胰腺的發(fā)育專題講座23The Beta Cell Lineage胰腺的發(fā)育專題講座The Alpha Cell Lineage胰腺的發(fā)育專題講座24The Alpha Cell Lineage胰腺的發(fā)育專題講Both Alpha and Beta Cells Derive From PDX-1 Expr

8、essing Precursors胰腺的發(fā)育專題講座25Both Alpha and Beta Cells DeriDirect evidence for the pancreatic lineage: NGN3+ cells are islet progenitors and are distinct from duct progenitorsGuoqiang Gu, Jolanta Dubauskaite and Douglas A. MeltonDevelopment 129, 2002Paper Two胰腺的發(fā)育專題講座26Direct evidence for the pancreG

9、eneration of The Transgenic Mice胰腺的發(fā)育專題講座27Generation of The Transgenic MPDX1+ Progenitors Give Rise to Islets, Acini and Duct in Adult MiceWild TypeHPAP-pdx1-creHPAP+pdx1-cre-ERpGK-creHPAP+pdx1-creLaz/VimentinE9.5/TM 8 week胰腺的發(fā)育專題講座28PDX1+ Progenitors Give Rise toNGN3+ Precursor Cells Give Rise to

10、Isletsngn3-cre胰腺的發(fā)育專題講座29NGN3+ Precursor Cells Give RisNGN3+ cells contribute to the renewal of islets in adult micengn3-cre-ER8 week TMDay 1Day 1Day 7Day 0胰腺的發(fā)育專題講座30NGN3+ cells contribute to the Notch Signaling Controls Multiple Steps of Pancreatic DifferentiationL. Charles Murtaugh, Ben Z. Stange

11、r, Kristen M. Kwan, and Douglas A. MeltonPNAS December 9, 2003Paper Three胰腺的發(fā)育專題講座31Notch Signaling Controls MultiNotch Signaling and Pancreas Developmentngn3X胰腺的發(fā)育專題講座32Notch Signaling and Pancreas DCre-activated misexpression of activated Notch1胰腺的發(fā)育專題講座33Cre-activated misexpression ofCre-activate

12、d misexpression of activated Notch1E9.5胰腺的發(fā)育專題講座34Cre-activated misexpression ofCre Activation By PDX1 Promoter 胰腺的發(fā)育專題講座35Cre Activation By PDX1 PromoteE13.5/TM E17.5E11.5/TM E15.5Cre Activation at E11.5-E13.5胰腺的發(fā)育專題講座36E13.5/TM E17.5E11.5/TCre Activation By Ngn3 PromoterE15.5E13.5胰腺的發(fā)育專題講座37Cre Ac

13、tivation By Ngn3 PromoteLate Cre ActivationAdult/TMControl胰腺的發(fā)育專題講座38Late Cre ActivationAdult/TMCon胰腺的發(fā)育專題講座培訓(xùn)課件A pulse-chase system for determining the origin of -cells 胰腺的發(fā)育專題講座40A pulse-chase system for deterSpecificity and Dose Dependence of RecombinationHPAP+胰腺的發(fā)育專題講座41Specificity and Dose Depe

14、ndencSpecificity and Dose Dependence of RecombinationHPAP+胰腺的發(fā)育專題講座42Specificity and Dose Dependenc-cells are formed by self-duplication of pre-existing -cellsHPAP+Insulin+D 0.14 M1 YD 0.16 M6-8 Week/TM 12 M胰腺的發(fā)育專題講座43-cells are formed by self-dup-cell Regeneration From Pre-existing -cells2 weeks af

15、ter 70% Ppx胰腺的發(fā)育專題講座44-cell Regeneration From Pre-eFission of pancreatic islets during postnatal growth of the mousePhilip A. Seymour, William R. Bennett and Jonathan M. W. SlackJ. Anatomy 204, 2004Paper Five胰腺的發(fā)育專題講座45Fission of pancreatic islets d23.17 X chromosomes undergo global changes. X染色體經(jīng)歷了

16、整體的變化.在真哺乳動物亞綱的細(xì)胞胚胎發(fā)育過程中,兩條X染色體中的其中一條會隨機(jī)地失活.在例外的情況下,自然界存在著大于2條的X染色體,除了一條外,其他都失活.胰腺的發(fā)育專題講座4623.17 X chromosomes undergo glXic(X染色體失活中心)是X染色體的順式作用區(qū)域,它是只讓一條X染色體保持活性的充分必要條件.Xic包括Xist基因,它編碼一個只在失活X染色體中發(fā)現(xiàn)的RNA.負(fù)責(zé)阻止Xist RNA在活性染色體上積累的機(jī)制還不明白.胰腺的發(fā)育專題講座47Xic(X染色體失活中心)是X染色體的順式作用區(qū)域,它是只讓在前體細(xì)胞中,一條X染色體的隨機(jī)失活引起X連鎖的成斑現(xiàn)象.陽性等位基因在活性染色體的細(xì)胞有野生表型;而陰性等位基因在活性染色體細(xì)胞有突變表型. 胰腺的發(fā)育專題講座48在前體細(xì)胞中,一條X染色體的隨機(jī)失活引起X連鎖的成斑現(xiàn)象.陽X染色體的失活涉及Xist RNA的穩(wěn)定,它能將失活染色體包被起來. 胰腺的發(fā)育專題講座49X染色體的失活涉及Xist RNA的穩(wěn)定,它能將失活染色體包X-gal staining for -gal activityin the intestinal cryptsHomozygous FemaleHetrozygous Female胰腺的發(fā)育專題講座50X-gal